the determination of the surface shear properties of adsorbed protein layers

Benjamins, J.; Vader, F. van Voorst

doi:10.1016/0166-6622(92)80271-3

Cited by 49 publications

(40 citation statements)

References 24 publications

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“…[27][28][29][30][31] Figure 3B shows the kinetics for DSAP adsorption in short times (less than 30s). For the highest DSAP concentration (3200 μg L -1 ), the surface tension falls down continuously, reaching 52 mN m -1 in 10 sec.…”

Section: Resultsmentioning

confidence: 99%

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Caseli¹,

Masui²,

Furriel³

et al. 2005

J. Braz. Chem. Soc.

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Este trabalho apresenta aspectos de equilíbrio e dinâmicos da adsorção na interface ar/líquido de duas formas de fosfatase alcalina de placa óssea de ratos: DSAP, solubilizada com tensoativo não-iônico (C 12 E 9 ), contendo uma âncora de glicosilfosfatidilinositol (GPI), e PLSAP, com a porção hidrofóbica da âncora clivada por fosfolipase-C. A tensão superficial dinâmica, γ dyn , e o módulo de elasticidade superficial dilatacional, ε, foram determinados para soluções de PLSAP, DSAP e C 12 E 9 pelo método de oscilação harmônica e análise do formato da gota eixo-simétrica. Cinéticas de adsorção revelaram que DSAP adsorve trinta vezes mais rapidamente que PLSAP, apresentando um mínimo, e, para PLSAP, a tensão superficial cai continuamente. Para o sistema DSAP/C 12 E 9 , ε atinge um máximo na concentração crítica de agregação (CAC), mas para PLSAP, ε diminui continuamente com a concentração. Soluções de C 12 E 9 apresentam ε mais elevados, decrescentes com a concentração. Um modelo, baseado na influência da âncora GPI, é proposto para explicar os resultados obtidos.This work presents equilibrium and dynamic aspects for the adsorption at the air/liquid interface of two rat osseous plate alkaline phosphatase forms: DSAP, solubilized by a surfactant, C 12 E 9 , and containing a glycosylphosphatidylinositol (GPI) anchor; and PLSAP, resulting from phospholipase-C cleavage of the hydrophobic portion of the GPI anchor. Dynamic surface tension, γ dyn , and surface elasticity modulus, ε, were determined for PLSAP, DSAP and pure C 12 E 9 solutions using harmonic oscillation and axisymmetric drop shape analysis Adsorption kinetics studies revealed that DSAP adsorbs thirty times faster than PLSAP, presenting a minimum in the curve. For DSAP/ C 12 E 9 mixed system, ε increases with concentration and a maximum appears at the critical aggregation concentration (CAC). For PLSAP, a continuous decreasing with concentration for γ dyn and ε was observed. For pure C 12 E 9 solution , the elasticity modulus increases with concentration and ε values are higher when compared to the mixed system. A model based on the influence of the GPI anchor is proposed.Keywords: dynamic surface tension, adsorption kinetics, dilatational surface elasticity, alkaline phosphatase, axisymmetric drop shape analysis IntroductionSurface elasticity is associated with the ability of a system to establish a new surface tension value due to a timely area variation. Considering that it is a nonequilibrium phenomenon, changes in surface tension occur either as a consequence of a sudden compression/ expansion of the interface, or due to a local concentration variation. Since elasticity values are associated with diffusion processes, adsorption/desorption, as well as the rearrangement of molecules at the interface, they depend on the time scale (or frequency) in which the perturbation is applied. In addition, these processes are influenced by molecular weight, shape and molecular interactions of the species present at the interface.Surface elasticity is directly relate...

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Section: Resultsmentioning

confidence: 99%

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Caseli¹,

Masui²,

Furriel³

et al. 2005

J. Braz. Chem. Soc.

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“…Fortunately, a number of methods have become available by which interfacial rheological behaviour can be studied under nonequilibrium conditions at intermediate strain rates both for oilewater and airewater interfaces (Benjamins & van Voorst Vader, 1992;Benjamis Cagna & Lucassen-Reynders, 1996;BerginkMartens, Bos, & Prins, 1993;Joos & Van Uffelen, 1995;Prins, 1995;Van Aken & Merks, 1996). Two developed techniques (the barrierand-plate and the dynamic drop tensiometer methods) are mainly used to determine the interfacial viscoelastic modulii.…”

Section: Introductionmentioning

confidence: 99%

Interfacial and emulsifying behaviour of crayfish protein isolate

Romero

Beaumal

David-Briand

et al. 2011

LWT - Food Science and Technology

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“…L-B films may provide useful models for the study of structural conformation of biological systems particularly those involving protein-membrane interactions. [21][22][23] In this article, both cast and L-B films of Ab (1-40) and (1-42) peptides were investigated by chiroptical methods using UCS-1.…”

Section: Introductionmentioning

confidence: 99%

CD measurements of β‐amyloid (1–40) and (1–42) in the condensed phase

Harada

Kuroda

2010

Biopolymers

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Circular dichroism (CD) spectroscopy of proteins/peptides in thin films can provide valuable information on the structures in the aggregated states; however, it is difficult to estimate the secondary structure content quantitatively due to artifact signals arising from macroscopic anisotropies which is unique to the solid phase. Using a Universal Chiroptical Spectrophotometer (UCS-1) together with the measurement and analytical procedures we have developed, we could obtain artifact-free CD spectra of cast and Langmuir-Blodgett (L-B) films of synthetic peptides, Aβ (1-40) and (1-42) which are related to Alzheimer's disease. The work gave insights into the mechanisms for structural transformation and amyloid-like aggregation.

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the determination of the surface shear properties of adsorbed protein layers

Cited by 49 publications

References 24 publications

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Adsorption kinetics and dilatational rheological studies for the soluble and anchored forms of alkaline phosphatase at the air/water interface

Interfacial and emulsifying behaviour of crayfish protein isolate

CD measurements of β‐amyloid (1–40) and (1–42) in the condensed phase

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