The determination of urinary testosterone using thin-layer chromatography and gas chromatography

Androgen metabolism in man. Some current concepts.

“…Normal values for the excretion of testosterone glucuronide have been obtained in Addison's disease (Ibayashi et al, 1964;Vermuelen, 1966), and consistently raised values of excretion and plasma-testosterone level and production have been found in congenital adrenal hyperplasia (Burger et al, 1964;Futterweit et al, 1964;Casey, 1965 ;Korenman et al, 1965;Lim and Dingman, 1965 ;Rosner et al, 1965;Degenhart et at., 1966) but the correlation with 17-oxosteroid excretion is not close; there is in this condition good suppression of testosterone with corticosteroid. Few observations are available in Gushing's syndrome with adrenal hyperplasia, but urinary testosterone glucuronide is increased (Ibayashi et al, 1964Vermuelen, 1966) and a high level of testosterone has been observed in the adrenal vein (Hudson et at., 1963) and a low level in the peripheral plasma of such a patient after adrenalectomy .…”

İdrarda Testosteron ve Epitestosteronun İnce Tabaka Kromatografisi ve Gaz Sıvı Kromatografisi İle Tayini

“…Yöntemin uygulanabilirliği normal kişilerle, ilaç alan kişi-lerin idrar örneklerinin analizi yapılarak gösterilmiştir. 1-Asit Hidroliz Yöntemi: Testosteron ve epitestosteron konjugatlarmın hidroliz ve ekstraksiyonunda GOTO ve FEHER'in yöntem-leri ile IBAYASHI'nin yöntemi kombine edilerek uygulanmıştır (18,19,20,21). 100 ml idrar, üzerine 0.5 ml formaldehit, 60 ml benzen ve 1 ml sülfürik asit ilave edilerek 2 saat 80-85°C'de geri çeviren soğu-tucu altında tutulmuştur.…”

“…Metanol azot gazı altında uçu-rulduktan sonra Kieselgel HF 254 ile kaplı plaklara standart testosteron asetat ve epitestosteron asetat ile beraber uygulanmıştır. Benzen-etilasetat (3:1) ile develope edildikten sonra 254 nm'de UV lambası altında standartlara karşılık gelen lekeler metanol ile elüe edilip daha sonra metanol uçurulmuştur (21). Kalıntıya iç standart olarak metiltestosteron ilave edilerek 5 µl gaz kromatografisine verilmiştir.…”

A Rapid and Practical Determination of Aldosterone in Urine by Double Isotope Method

“…In case even if it is oxidized, the monoacetate runs at the same Rf value as that of 21 deoxycorticosterone, and still separated from either aldosterone diacetate or tetrahydroaldosterone acetate. Of the improvements employed, some of Vol.18,No.6 RAPID URINE ALDOSTERONE DETERMINATION them have been already introduced individually by different investigators, e.g., an addition of dl-aldosterone diacetate as a carrier by Tait et al (1961), thin layer chromatography for steroid purification by Lisboa and Diczfalusy (1962) or for aldosterone by Nishikaze and Staudinger (1962), and, Kiesel gel HF 245 by Ibayashi et al (1964). Brodie et al (1967), applied these advantages to plasma aldosterone.…”