The development and ageing of membranes from etioplasts of <i>Avena sativa</i>

Bergweiler, Paul; Röper, Ursula

doi:10.1111/j.1399-3054.1984.tb06082.x

Cited by 18 publications

(9 citation statements)

References 19 publications

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“…Again, the predicted reductase sequence (Fig. 10) with its relatively high bl-sic amino acid content (Arg and Lys accounting for 120%) makes it possible to comprehend the high pI values (8.2-8.5) reported for the reductase (Ikeuchi & Marakami, 1982;Bergweiler et al, 1983). This basic property might also be considered in assigning a role for the reductase in the etioplast membrane.…”

Section: Discussionmentioning

confidence: 94%

Cloning and sequencing of protochlorophyllide reductase

Darrah

Kay

Teakle

et al. 1990

Biochemical Journal

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Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a A-phage gtl cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase. p127 codes for more than 95 %o of the reductase protein.

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Section: Discussionmentioning

confidence: 94%

Cloning and sequencing of protochlorophyllide reductase

Darrah

Kay

Teakle

et al. 1990

Biochemical Journal

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“…The etioplasts themselves may well be such a target. Indeed, Bergweiler et al, (1984), using isolated etioplasts and protein analysis, showed that, in the case of oat leaves, etioplast proteins were degraded during prolonged etiolation. In the present work, the step by step description of cotj'ledon energy metabolism has related degradation processes during etiolation with carbohydrate starvation, rather than with Intrinsic ageing or senescence of plastids.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate starvation is a major determinant of the loss of greening capacity in cotyledons of dark-grown sugar beet seedlings

Elamrani¹,

Gaudillère²,

Raymond³

1994

Physiol Plant

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1994, Carbohydrate starvation is a major determinant of the loss of greening capacity in cotyledons of dark-grown sugar beet seedlings, -Physiol, Plant, 91: 56-64.The transition of cotyledons from heterotrophy to autotrophy is a critical step of seedling establishment. We have studied the greening capacity of sugar beet (Beta vulgaris L. cv, Vega) cotyledons in relation to carbohydrate and energy metabohsm during dark growth. During early growth, sugar beet cotyledons behaved mainly as a lipid-mobilizing and gluconeogenic tissue providing substrates to the seedling. Reserve mobilization was followed by a maximum of the adenine nucleotide pool on day 6 in strict correlation with the maximum of greening capacity. This was immediately followed by the onset of a typical situation of carbohydrate starvation characterized by substrate limitation of respiration, a decrease in the adenine nucleotide pool and, as shown by the respiratory quotient and the loss of proteins, a probable utilization of cellular proteins and lipids to sustain respiration. The conversion from etioplast to chloroplast, as determined by the rate of chlorophyll synthesis, was less and less efficient as carbohydrate starvation continued, finally leading to incomplete and heterogeneous greening on day 12, The relationship of the loss of greening capacity with carbohydrate starvation is discussed.

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“…There is no distinct border between them in situ. Together they constitute a dynamic system where the relative amount of the two structures depends on plant species, age and growth conditions of the plant (Lutz 1981, Bergweiler et al 1984, Frances et al 1992. The lipid to protein ratio is higher in PLBs than in PTs, and so is the ratio of monogalactosyldiacylglycerol (MGDG) to digalactosyldiacylglycerol (DGDG; Sandelius 1984, Protoschill-Krebs andKesselmeier 1988).…”

Section: Prolamellar Bodies and Prothylakoidsmentioning

confidence: 98%

“…Two membrane structures can be seen in electron micrographs of etioplasts, the prolamellar bodies (PLBs), which are composed of highly regular networks of tubular membranes, and the lamellar-like prothylakoids (PTs). During continuous irradiation the PLBs will disappear and normal thylakoids will form in 24 to 96 h (Boardman and Anderson 1978), if the plants are not too old (Bergweiler et al 1984, Rascio et al 1984). There are a number of excellent reviews within the field (e.g.…”

Section: Introductionmentioning

confidence: 96%

With chlorophyll pigments from prolamellar bodies to light-harvesting complexes

Sundqvist¹,

Dahlin²

1997

Physiol Plant

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With chlorophyll pigments from prolamellar bodies to light-harvesting complexesChrister Sundqvist and Clas Dahlin Sundqvist, C. and Dahlin, C, 1997. With chlorophyll pigments from prolamellar bodies to light-harvesting complexes. -Physiol. Plant 100: 748-759.The biosynthetic chain leading from 5-aminolevulinic acid to chlorophyll is localised to the plastid. Many of the enzymes are nuclear-encoded. NADPH-protochlorophyllide oxidoreductase (EC 1.3.1.33) is one such enzyme which is encoded by two different genes and can exist in an A and a B form. Its import into the plastid seems to be facilitated when protochlorophyllide is present in the chloroplast envelope. Within the plastid the reductase is assembled to thylakoids or prolamellar bodies. The specific properties of the reductase together with the specific properties of the lipids present in the etioplast inner membranes promote the formation of the three-dimensional regular network of the prolamellar bodies. The reductase forms a temary complex with protochlorophyllide and NADPH that gives rise to different spectral forms of protochlorophyllide. Light transforms protochlorophyllide into chlorophyllide and this photoreaction induces a conformational change in the reductase protein which leads to a process of disaggregation of enzyme, pigment aggregates and membranes, which can be followed spectroscopically and with electron microscopy. The newly formed chlorophyllide is esterified by a membrane-bound nuclear-encoded chlorophyll synthase and the chlorophyll molecule is then associated with proteins into active pigment protein complexes in the photosynthetic machinery.

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The development and ageing of membranes from etioplasts of Avena sativa

Cited by 18 publications

References 19 publications

Cloning and sequencing of protochlorophyllide reductase

Cloning and sequencing of protochlorophyllide reductase

Carbohydrate starvation is a major determinant of the loss of greening capacity in cotyledons of dark-grown sugar beet seedlings

With chlorophyll pigments from prolamellar bodies to light-harvesting complexes

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