The development and function of γδ T cells

Haas, Werner; Kaufman, Stefan; Martínez, Carlos A.

doi:10.1016/0167-5699(90)90133-t

Cited by 76 publications

(38 citation statements)

References 39 publications

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“…Thus, the enhanced proportion of CD4' T cells in patients with high viral replication suggests that CD4' T cells may provide helper effects to potential cytotoxic 7/i T cells or CD57'^ cells. Indeed, T lymphocytes expressing the 7/^ receptor have been shown to mediate non-MHC-restricted cytotoxicity [34]. Moreover, natural killer (NK) cells which display particular phenotypic [35] and functional characteristics are also known to mediate non-MHC-restricted cytotoxic reactions, notably against infectious agents [36].…”

Section: Discussionmentioning

confidence: 99%

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

Pham

Martinot‐Peignoux

Mosnier

et al. 1995

Clinical and Experimental Immunology

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SUMMARYThe pathogenic mechanisms that lead to chronic hepatitis C are unknown. As hepatitis C virus (HCV) has been shown to induce T cell response, we assessed whether a partictilar T lymphocyte subset could be preferentially detected in theliver of patients with chronic hepatitis C in relation to viraemia or HCV genotypes. The immunophenotypes of liver-derived lymphocytes were analysed in 26 patients by flow cytometry and immunohistochemistry. Viraemia was quantified by branched DNA assay. Using this assay, HCV RNA was not detectable in six patients. HCV RNA was detected in 20 patients, and titres ranged from 8 to 137 x 10''Eq/ml. Genotyping was performed using a line probe assay. Type la. lb. 2a, 3a and 4a were found to infect 2. 10. 2, 7 and 3 patients, respectively. The CD4 ^ /CD8 * ratio of liver-derived lymphocytes was significantly higher [P < 001) in patients with detectable viraemia than in patients without detectable viraemia. In contrast, neither the percentage of ^/i^ T lymphocytes nor that of CD2^CD57^ cells was different in the groups. When comparing the CD4^/CD8^ ratio, the percentage of-y/^ T lymphocytes or CD2^CD57' cells according to genotype, the differences were not significant. These results suggest that the CD4^/ CD8"^ratio of liver-derived lymphocytes is related to viraemia but not to HCV genotypes in patients with chronic hepatitis C. and that T lymphocytes may be involved in the pathogenesis of liver le.sions in chronic hepatitis C, Keywords hepatitis C virus chronic hepatitis C liver-derived lymphocytes lymphocyte subsets viraemia hepatitis C virus genotype

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Section: Discussionmentioning

confidence: 99%

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

Pham

Martinot‐Peignoux

Mosnier

et al. 1995

Clinical and Experimental Immunology

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“…This cDNA coded a polypeptide designated as c, which was linked to a second polypeptide called d to form the TCR-cd (1). Accordingly, two different subsets of T-lymphocytes are known at present, depending on the type of membrane antigen-receptor they express: ab T-lymphocytes that express the ab-TCR antigen receptor and cd T-lymphocytes (cd LT) that express the cd-TCR antigen receptor (2)(3)(4)(5). Not much is known about cd T-lymphocytes, partly due to their more recent discovery, although since then, several immunological studies have tried to differentiate between the two T-lymphocyte subsets, with special emphasis on cd LT due to their outstanding functions (6).…”

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confidence: 99%

Values for αβ and γδ T‐lymphocytes and CD4+, CD8+, and CD56+ subsets in healthy adult subjects: Assessment by age and gender

Andreu‐Ballester

García‐Ballesteros

Benet-Campos

et al. 2012

Cytometry Part B Clinical

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Background: Normal reference values in healthy subjects for T-lymphocytes for both types of receptors, ab and cd, and their subsets are yet to be defined. The aim of this study was to measure peripheral blood ab and cd total T-lymphocytes and their subsets in a population of healthy subjects, in order to obtain valid reference values for studies in human pathology.Methods: We studied a total of 157 healthy subjects, 78 men and 79 women, establishing their levels of CD31, CD41, CD81, CD561, abCD31, abCD31CD41, abCD31CD81, abCD31CD561, cdCD31, cdCD31CD42CD82, cdCD31CD81, and cdCD31CD561 T-cells by flow cytometry. The T-cell subsets were compared for different age and gender groups.Results: A significant decrease in CD31, CD31CD41, CD31CD41 ab, and CD31 cd T-cells was observed in elderly subjects. CD31, CD31 ab, and CD31CD41 ab T-cells increased in women, while CD31CD561 ab T-cells increased in men.Conclusions. These reference values could be useful in further research studies for assessing changes that occur in the different ab and cd T subsets in human pathology. V C 2012 International Clinical Cytometry Society

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“…Typically, 2-5% of lymphocytes in the peripheral blood of adults express the ␥␦ TCR. V␥9V␦2 T cells constitute the majority of ␥␦ T cells in peripheral blood, tonsils, and spleen (1,2). Several observations have shown that ␥␦ T cells dramatically increase in blood of patients infected with Mycobacterium tuberculosis (3), Brucella melitensis (4), Francisella tularensis (5), Listeria monocytogenes (6), Leishmania donovani (7), and Plasmodium falciparum (8).…”

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confidence: 99%

Functional and Structural Similarity of Vγ9Vδ2 T Cells in Humans and Aotus Monkeys, a Primate Infection Model for Plasmodium falciparum Malaria

Daubenberger

Salomon

Vecino

et al. 2001

The Journal of Immunology

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γδ T cells are implicated to play crucial roles during early immune responses to pathogens. A subset of human γδ T cells carrying the Vγ9Vδ2 TCR recognize small, phosphorylated nonpeptidic Ags. However, the precise role of these cells and the ligands recognized in human immune responses against pathogens remains unclear because of the lack of suitable animal models. We have analyzed the reactivity of spleen cells of the New World monkey Aotus nancymaae against isopentenyl pyrophosphate (IPP), a phosphorylated microbial metabolite selectively activating Vγ9Vδ2 T cells. Spleen cells were stimulated by IPP and the expanding cell population expressed the Vγ9 TCR. TRGV-J and TRDV-D-J rearrangements expressed by IPP-stimulated cells of Aotus were analyzed by RT-PCR and DNA sequencing. The TRGV-J and TRDV-D-J rearrangements expressed by IPP-stimulated Aotus and human γδ T cells were similar with respect to 1) TCR gene segment usage, 2) a high degree of germline sequence homology of the TCR gene segments used, and 3) the diversity of the CDR3 regions. Phylogenetic analysis of human, Pan troglodytes, and A. nancymaae TRGV gene segments showed that the interspecies differences are smaller than the intraspecies differences with TRGV9 gene segments located on a distinct clade of the phylogenetic tree. The structural and functional conservation of Vγ9Vδ2 T cells in A. nancymaae and humans implicates a functionally important and evolutionary conserved mechanism of recognition of phosphorylated microbial metabolites.

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The development and function of γδ T cells

Cited by 76 publications

References 39 publications

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C

Values for αβ and γδ T‐lymphocytes and CD4+, CD8+, and CD56+ subsets in healthy adult subjects: Assessment by age and gender

Functional and Structural Similarity of Vγ9Vδ2 T Cells in Humans and Aotus Monkeys, a Primate Infection Model for Plasmodium falciparum Malaria

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