The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of <i>Buddleja lindleyana</i> Fort.

Zhang, Xia; Zhang, Zhiqing; Zhang, Li-cang; Wang, Kexin; Zhang, Lantong; Li, Deqiang

doi:10.1039/d1ra04154a

Cited by 9 publications

(12 citation statements)

References 42 publications

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“…For the isolation of polyphenols from animal biological matrices, various methods were applied based mainly on liquid-liquid extraction [17], solid phase extraction [15] and protein precipitation [9][10][11][12][13][14]16]. In our method, simple protein precipitation with acetonitrile was applied with further solvent evaporation and reconstitution in methanol:water mobile phase.…”

Section: Uplc-ms/ms Analysismentioning

confidence: 99%

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Application of UPLC-MS/MS Method for Analysis of Apigenin, Apigenin 7-Glucoside and Chlorogenic Acid in Goat Serum

et al. 2023

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UPLC-MS/MS method was developed for determination of bioactive compounds including chlorogenic acid (CA), apigenin (AP) and apigenin 7-glucoside (AP7G) in goat serum. The analytes were separated on Kinetex C18 analytical column and a mobile phase consisting 0.1% formic acid in water and methanol with gradient elution. Detection of analytes was performed using a triple quadrupole mass spectrometer with electrospray ionization in positive mode. The lower limit of quantitation was 0.5 ng mL–1 for AP and AP7G and 2.5 ng mL–1 for CA. The inter- and intra-day precision of the method expressed as relative standard deviation was in the range of 1.22–14.5%. The accuracy of the method expressed as the relative error was in the range of 0.147–14.6%. The extraction recoveries of the compounds were 76.2–89.5%. The validated method was successfully applied for analysis of the compounds in the serum of sixty goats, half of which were fed with fodder with the addition of an herbal mixture. The higher concentration values of all analyzed compounds were reported in the serum of the animals fed with herbs compared to the control group receiving standard fodder.

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Section: Uplc-ms/ms Analysismentioning

confidence: 99%

“…Up to now, there is no study on the AP, AP7G and CA concentrations in goats. Nevertheless, several reports are available regarding the analysis of the polyphenolic compounds in other animal species, including rats [9][10][11][12][13][14][15], and mice [16,17]. In these reports, validated Fig.…”

Section: Introductionmentioning

confidence: 99%

Application of UPLC-MS/MS Method for Analysis of Apigenin, Apigenin 7-Glucoside and Chlorogenic Acid in Goat Serum

et al. 2023

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“…A reference for the study of the traceability of the material basis of the efficacy of QBD needs to be provided along with technical support and data for its quality standard 3 . In recent years, high‐performance LC–tandem MS (HPLC‐MS/MS) has been routinely used to determine the content of the compounds present in herbal medicines 4 . The main advantage of MS/MS is the ability to detect a wide range of drugs with high sensitivity and specificity at the time of analysis 4 .…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, high‐performance LC–tandem MS (HPLC‐MS/MS) has been routinely used to determine the content of the compounds present in herbal medicines 4 . The main advantage of MS/MS is the ability to detect a wide range of drugs with high sensitivity and specificity at the time of analysis 4 . The HPLC‐MS/MS technique is rapid and highly efficient.…”

Section: Introductionmentioning

confidence: 99%

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Qualitative and quantitative analysis of the major components in Qinghao Biejia decoction by UPLC‐Orbitrap Fusion‐MS/MS and UPLC‐QQQ‐MS/MS and evaluation of their antibacterial activities

Cheng

Han

et al. 2022

Phytochemical Analysis

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Objective: In the present study, the chemical components of Qinghao Biejia decoction (QBD) were qualitatively and quantitatively analyzed using UPLC-Orbitrap Fusion-MS/MS and UPLC-QQQ-MS/MS techniques, followed by identification of each component's origin and evaluation of the antibacterial activity of QBD and its components.Methods: High-resolution mass spectrometry was used to obtain information on the precise molecular weight, retention time, and fragmentation ion peaks of the compounds used to identify the components of QBD and establish a method for their quantification. In vitro assays including determination of the minimal inhibitory concentration and growth curves were used to assess the antibacterial activity of QBD and its components.Results: A total of 39 components, including fatty acids, phenolic acids, amino acids, flavonoids, coumarins, terpenoids, and alkaloids, were identified by UPLC-Orbitrap Fusion-MS/MS. A high-performance analytical method was also established to quantify 12 components of QBD. The content of mangiferin was relatively high (estimated to be 814 μg/g). The results of the antibacterial assays indicated that mangiferin exhibits antibacterial effects against two strains causing respiratory tract infections. Conclusions:The present study suggests that mangiferin may serve as a natural compound which shows high antibacterial activity. The results can aid the discovery and analysis of the active antimicrobial components present in QBD and further provide a reference for quality assessment of multi-component herbal prescriptions.

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A comprehensive study of Chuanwang Xiaoyan capsule based on characterization of chemical constituents in vitro and in vivo using ultra‐high‐performance liquid chromatography‐quadrupole‐Exactive Orbitrap mass spectrometry and pharmacokinetic study of multiple components in rats using ultra‐high‐performance liquid chromatography‐triple quadrupole‐tandem mass spectrometry

Wang,

Gao

et al. 2024

Biomedical Chromatography

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Chuanwang Xiaoyan (CWXY) capsule is primarily used to treat a variety of acute and chronic inflammations, including acute and chronic pharyngitis and tonsillitis. However, a systematic study of its chemical constituents is still not available. This study evaluated the chemical constituents in vitro and metabolite profiles in vivo of CWXY using ultra‐high‐performance liquid chromatography (UHPLC) coupled with Q‐Exactive Orbitrap mass spectrometry, and the pharmacokinetic behaviors of the nine main components in rats were detected using ultra‐high‐performance liquid chromatography‐triple quadrupole‐mass spectrometry (UPLC‐QQQ‐MS/MS). A total of 92 chemical constituents in CWXY were preliminarily identified in vitro. After oral administration to rats, 56 prototype components and 128 metabolites of CWXY were detected in the biological samples of rat plasma, urine, bile, and feces. Of these prototype components and metabolites, seven new compounds, namely M15, M16, M25, M30, M31, M71, and M128, were detected for the first time. The quantitation method of nine components in rat plasma was developed using a pharmacokinetic study. To the best of our knowledge, this study was the first to investigate the pharmacokinetic behavior of triumbelletin.

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The development and validation of a sensitive HPLC-MS/MS method for the quantitative and pharmacokinetic study of the seven components of Buddleja lindleyana Fort.

Abstract: Quality control and pharmacokinetics of Buddleja lindleyana Fort by HPLC-MS/MS.

Cited by 9 publications

References 42 publications

Application of UPLC-MS/MS Method for Analysis of Apigenin, Apigenin 7-Glucoside and Chlorogenic Acid in Goat Serum

Application of UPLC-MS/MS Method for Analysis of Apigenin, Apigenin 7-Glucoside and Chlorogenic Acid in Goat Serum

Qualitative and quantitative analysis of the major components in Qinghao Biejia decoction by UPLC‐Orbitrap Fusion‐MS/MS and UPLC‐QQQ‐MS/MS and evaluation of their antibacterial activities

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