The calibration and validation of input parameters in the Discrete Element Method (DEM) are crucial for accurately simulating physical processes, typically achieved through experimental particle behavior analysis. Enhancing the accuracy of DEM models allows for more reliable predictions of material behavior, which is essential for optimizing engineering applications that involve particulate materials. In this study, we present a methodology for analyzing the movement properties of particulate materials, employing a combination of Caliscope software to obtain the real-world co-ordinates based on pixel values from both cameras and artificial neural networks for regression as straightforward and efficient tools. This approach enables the validation and calibration of digital twins of particulate matter systems with respect to motion characteristics. The method of contrast points was utilized to acquire spatial co-ordinates of particulate material movement from experimental measurements, facilitating precise trajectory determination and the subsequent verification of simulation predictions. The neural network analysis demonstrated high accuracy, achieving R2 values of 0.9988, 0.9972, and 0.9982 for the X–, Y–, and Z–axes, respectively. The standard deviation between the predicted and actual co-ordinates was found to be 1.8 mm. A comparative analysis of particle trajectories from both the model and experimental data indicated strong agreement, underscoring the soundness and reliability of this approach.