The Development of a Highly Sensitive Fiber-Optic Oxygen Sensor

Chu, Cheng-Shane; Syu, J. C.

doi:10.3390/inventions1020009

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…24 Porphyrin dyes, known for their high brightness and sensitivity to oxygen, are commonly used in oxygen sensing studies. 46 Paper Analyst PdTFPP in particular offers a wide range of oxygen detection from 0 to 100% oxygen and is compatible with standard DAPI excitation and emission filters, 47 and PdTFPP films exhibit a linear and highly sensitive response to changes in oxygen tension. 24 Here, we prepared an oxygen sensor by spin-coating PdTFPP dye in polystyrene onto a glass coverslip.…”

Section: Analytical Set Up and Sensor Characterizationmentioning

confidence: 99%

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

Anbaei,

Stevens,

Ball

et al. 2024

Analyst

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Section: Analytical Set Up and Sensor Characterizationmentioning

confidence: 99%

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

Anbaei,

Stevens,

Ball

et al. 2024

Analyst

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“…24 Porphyrin dyes, known for their high brightness and sensitivity to oxygen, are commonly used in oxygen sensing studies. 42 PdTFPP in particular offers a wide range of oxygen detection from 0 to 100% oxygen and is compatible with standard DAPI excitation and emission filters, 43 and PdTFPP films exhibit a linear and highly sensitive response to changes in oxygen tension. 24 Here, we prepared an oxygen sensor by spin-coating PdTFPP dye in polystyrene onto a glass coverslip.…”

Section: Analytical Set Up and Sensor Characterizationmentioning

confidence: 99%

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

Anbaei,

Stevens,

Ball

et al. 2024

Preprint

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Cellular metabolism has been closely linked to activation state in cells of the immune system, and the oxygen consumption rate (OCR) in particular serves as a valuable metric for assessing metabolic activity. Several oxygen sensing assays have been reported for cells in standard culture conditions. However, none have provided a spatially resolved, optical measurement of local oxygen consumption in intact tissue samples, making it challenging to understand regional dynamics of consumption. Therefore, here we established a system to monitor the rates of oxygen consumption in ex vivo tissue slices, using murine lymphoid tissue as a case study. By integrating an optical oxygen sensor into a sealed perfusion chamber and incorporating appropriate correction for photobleaching of the sensor and of tissue autofluorescence, we were able to visualize and quantify rates of oxygen consumption in tissue. This method revealed for the first time that the rate of oxygen consumption in naive lymphoid tissue was higher in the T cell region compared to the B cell and cortical regions. To validate the method, we measured OCR in the T cell regions of naive lymph node slices using the optical assay and estimated the consumption rate per cell. The predictions from the optical assay were similar to reported values and were not significantly different from those of the Seahorse metabolic assay, a gold standard method for measuring OCR in cell suspensions. Finally, we used this method to quantify the rate of onset of tissue hypoxia for lymph node slices cultured in a sealed chamber and showed that continuous perfusion was sufficient to maintain oxygenation. In summary, this work establishes a method to monitor oxygen consumption with regional resolution in intact tissue explants, suitable for future use to compare tissue culture conditions and responses to stimulation.

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“…The polarization of the electrodes and measurement of the variation of the output parameter is dependent on the sensing approaches, which could be potentiometric, voltametric, conductimetric, or amperometric. A filter, between the membrane and the environment gases, can be used to limit contact with unwanted gases, improving accuracy and sensitivity on these devices [31,90,92,[115][116][117]. The representation of an amperometric electrochemical gas sensor is shown in Figure 2.…”

Section: Electrochemicalmentioning

confidence: 99%

“…The polymer matrix, with the target gas and adequate wavelength and energy, will react and emit a different wavelength, where the intensity of the light is proportional to the gas concentration. The response time is variable, being approximately a few seconds, depending on the chosen materials, intensity of the light source and target gas concentrations [28,94,97,115]. Figure 6 illustrates this technique of sensing gases.…”

Section: Opticmentioning

confidence: 99%

IoT-Enabled Gas Sensors: Technologies, Applications, and Opportunities

Gomes

Rodrigues

Rabêlo

et al. 2019

JSAN

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Ambient gas detection and measurement had become essential in diverse fields and applications, from preventing accidents, avoiding equipment malfunction, to air pollution warnings and granting the correct gas mixture to patients in hospitals. Gas leakage can reach large proportions, affecting entire neighborhoods or even cities, causing enormous environmental impacts. This paper elaborates on a deep review of the state of the art on gas-sensing technologies, analyzing the opportunities and main characteristics of the transducers, as well as towards their integration through the Internet of Things (IoT) paradigm. This should ease the information collecting and sharing processes, granting better experiences to users, and avoiding major losses and expenses. The most promising wireless-based solutions for ambient gas monitoring are analyzed and discussed, open research topics are identified, and lessons learned are shared to conclude the study.Technologies to sense gases and wireless communications support are elaborated in Sections 5 and 6, respectively. The most promising solutions in terms of sensing methods and IoT-enabled solutions are discussed in Section 7 and open research topics are identified. Lessons learned are shared at Section 8 and, finally, Section 9 concludes the study. Background on Environmental GasesSome gases are the key to ensure the functionality of systems and entire industries, as well as the presence of other gases being a problem in other fields, causing the loss of entire production lines, as in the food industry, or even cause the loss of lives and explosions. In this section, the most important gases in terms of pollution monitoring and control, health issues, and accident prevention are listed with their main characteristics.Oxygen (O 2 ) is the most important gas for life, and is crucial in numerous fields. Patients under anesthesia, or recovering from surgery and from certain diseases need controlled O 2 doses to keep them alive and fully recovered. The decrease of oxygen levels in enclosed spaces can be related with other gases leakage, which would lead people inside these spaces to asphyxiate, leading to an unconscious state, or even to death [29]. Numerous industrial processes rely on the correct concentration of this gas to achieve the best results, mainly chemical and combustion, which without the correct percentages will not grant the best performance of systems. Engine control systems depend on the correct mixture to achieve the expected performances, whether it is lower fuel consumption or power and speed [29][30][31][32].Carbon dioxide (CO 2 ) is a colorless, odorless gas, generated by the oxidation and combustion of hydrocarbon, as well as by living beings in the respiration process. It is a key gas for the greenhouse effect, and the increase of its levels, in the presence of other gases, is related with atmospheric pollution. It is also the key gas on the oxygen production by the photosynthesis process. The accumulation of this gas in enclosed spaces can be responsible to s...

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The Development of a Highly Sensitive Fiber-Optic Oxygen Sensor

Cited by 5 publications

References 29 publications

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices

IoT-Enabled Gas Sensors: Technologies, Applications, and Opportunities

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