The development of a meningococcal disease vaccine based on outer membrane vesicles

Gorringe, Andrew; Halliwell, Denise; Matheson, Mary; Reddin, Karen M.; Finney, Michelle; Hudson, Michael J.

doi:10.1016/j.vaccine.2005.01.055

Cited by 51 publications

(19 citation statements)

References 8 publications

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“…We have previously shown that a vaccine based on N. lactamica OMVs protects against lethal challenge in a mouse model of meningococcal disease. 15,16 Here we present data to identify immunogenic components and characterize immune responses to the vaccine in laboratory animals. These pre-clinical data will allow a better understanding of the vaccine composition and immune responses elicited ahead of clinical evaluation.…”

Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

“…The good antibody response (as detected by ELISA) but lack of in vitro bactericidal activity in sera from mice and rabbits immunized with N. lactamica OMVs or N. meningitidis OMVs, is consistent with the suggestion that other protective mechanisms, such as opsonophagocytosis, may provide the strong cross-reactive protection seen previously with a mouse model of meningococcal disease, in which antibodies raised against N. lactamica OMVs protected against challenge with any of a range of serogroup B and C strains despite absence of any bactericidal responses. 15,16 An antibody response mediating opsonophagocytosis was detected in rabbits immunized with N. lactamica OMVs, but not in mice. This observation requires further investigation and may be due to incompatibility between mouse antibodies against these antigens and human phagocytic cells used in this assay.…”

Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

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Characterization of the key antigenic components and pre-clinical immune responses to a meningococcal disease vaccine based onNeisseria lactamicaouter membrane vesicles

Finney¹,

Vaughan²,

Taylor³

et al. 2008

Human Vaccines

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Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 99%

Characterization of the key antigenic components and pre-clinical immune responses to a meningococcal disease vaccine based onNeisseria lactamicaouter membrane vesicles

Finney¹,

Vaughan²,

Taylor³

et al. 2008

Human Vaccines

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“…Nonetheless, meningococcal disease is a global phenomenon which, in some settings, occurs in large outbreaks [22]. The idea that the colonisation of children with N. lactamica plays a role in the development of immunity to the meningococcus [23-25] has further stimulated comparative investigations of these two organisms and anti-meningococcal vaccines based N. lactamica have been proposed at various times [26,27]. …”

Section: Introductionmentioning

confidence: 99%

Independent evolution of the core and accessory gene sets in the genus Neisseria: insights gained from the genome of Neisseria lactamica isolate 020-06

et al. 2010

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BackgroundThe genus Neisseria contains two important yet very different pathogens, N. meningitidis and N. gonorrhoeae, in addition to non-pathogenic species, of which N. lactamica is the best characterized. Genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally.ResultsNon-pathogenic N. lactamica exhibits very similar population structure and levels of diversity to the meningococcus, whilst gonococci are essentially recent descendents of a single clone. All three species share a common core gene set estimated to comprise around 1190 CDSs, corresponding to about 60% of the genome. However, some of the nucleotide sequence diversity within this core genome is particular to each group, indicating that cross-species recombination is rare in this shared core gene set. Other than the meningococcal cps region, which encodes the polysaccharide capsule, relatively few members of the large accessory gene pool are exclusive to one species group, and cross-species recombination within this accessory genome is frequent.ConclusionThe three Neisseria species groups represent coherent biological and genetic groupings which appear to be maintained by low rates of inter-species horizontal genetic exchange within the core genome. There is extensive evidence for exchange among positively selected genes and the accessory genome and some evidence of hitch-hiking of housekeeping genes with other loci. It is not possible to define a 'pathogenome' for this group of organisms and the disease causing phenotypes are therefore likely to be complex, polygenic, and different among the various disease-associated phenotypes observed.

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“…The isolation of OMV starts from culture supernatant after removal of cultured bacteria by centrifugation at 6,000 × g for 5 min. Supernatant was filtered via 0.22 µm sterilization vacuum filter (V25) [83]. The non-OMV proteins are removed by filtration using the cut-off of 50-100 kDa membranes before ultracentrifugation.…”

Section: Isolation Purification and Quantification Of Omv And Evmentioning

confidence: 99%

Significances of OMV and Extracellular Vesicle Proteomics

Tiwari¹

2017

J Data Mining Genomics Proteomics

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Outer Membrane Vesicle (OMV) proteome has been involved into the pathogenesis of diseases and resistance of microorganisms against a number of antibiotics, mechanism of action of probiotics and host-pathogen interaction etc. We have enlightened the role played by extracellular vesicles proteomics in the pathogenesis of different diseases related to human. Isolation succeeded by purification of ample amount of OMV from biological samples, is one of the most important steps for further proteome related analysis. With the development of both labelled and label-free methods used in proteomics, significant progress has been made in previous years in membrane proteomics. Hence, it is important to review the biological significance of proteins found in the OMV fractions using membrane proteomics approach. We have also explained methods used for isolation, purification and quantification of OMV. In the present review, it can be concluded that proteomic study of outer membrane and extracellular vesicles has now gained priority for the detailed study of disease pathogenesis, drug resistance, vaccine development, cell signalling etc.

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The development of a meningococcal disease vaccine based on outer membrane vesicles

Cited by 51 publications

References 8 publications

Characterization of the key antigenic components and pre-clinical immune responses to a meningococcal disease vaccine based onNeisseria lactamicaouter membrane vesicles

Characterization of the key antigenic components and pre-clinical immune responses to a meningococcal disease vaccine based onNeisseria lactamicaouter membrane vesicles

Independent evolution of the core and accessory gene sets in the genus Neisseria: insights gained from the genome of Neisseria lactamica isolate 020-06

Significances of OMV and Extracellular Vesicle Proteomics

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