The development of a new disposable pipette extraction phase based on polyaniline composites for the determination of levels of antidepressants in plasma samples

Chaves, Andréa Rodrigues; Moura, B.H.F.; Caris, Juciene Aparecida; Rabelo, D.; Queiroz, Maria Eugênia Costa

doi:10.1016/j.chroma.2015.04.027

Cited by 37 publications

(9 citation statements)

References 22 publications

(33 reference statements)

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“…In addition, it allows for automated and high-throughput preparation when combined with the liquid handling system [18,19]. This sample preparation method has been widely used in the analysis of drugs [14,[18][19][20][21][22][23][24], pesticide residues [16,17,[25][26][27][28][29][30], heavy metal ions [31], and environmental contaminants [32][33][34][35]. In the present work, the parameters of DPX were systematically investigated on the clean-up of neonicotinoid pesticides in bee pollen.…”

Section: Introductionmentioning

confidence: 99%

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Chen

2020

Molecules

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As the main source of nutrients for the important pollinator honeybee, bee pollen is crucial for the health of the honeybee and the agro-ecosystem. In the present study, a new sample preparation procedure has been developed for the determination of neonicotinoid pesticides in bee pollen. The neonicotinoid pesticides were extracted using miniaturized salting-out assisted liquid-liquid extraction (mini-SALLE), followed by disposable pipette extraction (DPX) for the clean-up of analytes. Effects of DPX parameters on the clean-up performance were systematically investigated, including sorbent types (PSA, C18, and silica gel), mass of sorbent, loading modes, and elution conditions. In addition, the clean-up effect of classical dispersive solid-phase extraction (d-SPE) was compared with that of the DPX method. Results indicated that PSA-based DPX showed excellent clean-up ability for the high performance liquid chromatography (HPLC) analysis of neonicotinoid pesticides in bee pollen. The proposed DPX method was fully validated and demonstrated to provide the advantage of simple and rapid clean-up with low consumption of solvent. This is the first report of DPX method applied in bee pollen matrix, and would be valuable for the development of a fast sample preparation method for this challenging and important matrix.

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Section: Introductionmentioning

confidence: 99%

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Chen

2020

Molecules

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“…DPX Extraction Phase Development. As mentioned on introduction section, the synthesis of Sty-DVB/PANI composite was based on our previously published work [8], however the developed composite in that case, did not exhibit adequate chemical stability and reproducibility between extractions cycles. In order to improve the extraction phase, the synthesis protocol has been modified resulting in the pernigraniline composite which has an over oxidation state.…”

Section: Resultsmentioning

confidence: 99%

“…These techniques are laborious and time consuming and require large amounts of organic solvents. The trends in analytical chemistry point toward methods that lead to simplification, miniaturization of analytical instrumentation and sample preparation techniques, and reduction of organic solvent and sample volumes [8].…”

Section: Introductionmentioning

confidence: 99%

“…Recently our group presented a DPX extraction phase based on styrene-divinylbenzene (Sty-DVB) copolymer and polyaniline (PANI) composites, which was suitable for the antidepressants (fluoxetine and norfluoxetine) in plasma samples by highperformance liquid chromatography and fluorescence detection (HPLC-FD) [8]. The developed DPX method is shown to be very rapid, taking just a few minutes to perform without any solvent evaporation.…”

Section: Introductionmentioning

confidence: 99%

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Disposable pipette extraction phase based on styrene–divinylbenzene/pernigraniline composite, applied for dexamethasone determination in synovial fluid by HPLC with UV detector

Soares

Alvarenga

Ruggiero

et al. 2019

Acta Chromatographica

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A reliable method using disposable pipette extraction (DPX) based on composite of pernigraniline and styrenedivinylbenzene (Sty-DVB) copolymer was applied to the analysis of dexamethasone in synovial fluid using highperformance liquid chromatography and ultraviolet (UV) detector (HPLC-UV). DPX variables, namely, number of draw/eject cycles for extraction or desorption, sample pH, volume, and desorption solvent, were optimized to establish the best sorption equilibrium and analysis time. The highest extraction efficiency value was obtained with 50 μL of synovial fluid mixed with 1950 μL of water, in five cycles of 300 μL of sampling, followed by liquid desorption of the drug with 300 μL of methanol in three cycles. The developed method demonstrated a linear response over the range from 10 to 100 ng/mL, with R 2 = 0.993. The limit of quantification (LOQ) was 10 ng/mL. Based on the validation results, the proposed method can be a useful tool to detect dexamethasone levels in synovial fluid.

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“…Convencionalmente, as técnicas de preparo de amostra utilizando precipitação de proteínas ("protein precipitation"-PPT), extração líquido-líquido ("liquid-liquid extraction" -LLE) e extração em fase sólida ("solid-phase" extraction SPE) são as mais utilizadas em bioanálise (CHAVES et al, 2015;KOLE et al, 2011;NOVÁKOVÁ;VLCKOVÁ, 2009a).…”

Section: Introdução Geralunclassified

Desenvolvimento de fases extratoras seletivas para técnicas de microextração para a determinação de fármacos e endocanabinóide em amostras de plasma por LC-MS/MS

Melo¹

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The development of a new disposable pipette extraction phase based on polyaniline composites for the determination of levels of antidepressants in plasma samples

Cited by 37 publications

References 22 publications

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

Disposable pipette extraction phase based on styrene–divinylbenzene/pernigraniline composite, applied for dexamethasone determination in synovial fluid by HPLC with UV detector

Desenvolvimento de fases extratoras seletivas para técnicas de microextração para a determinação de fármacos e endocanabinóide em amostras de plasma por LC-MS/MS

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