The development of an electrochemical DNA biosensor based on quercetin as a new electroactive indicator for DNA hybridization detection

Alipour, Esmaeel; Norouzi, Sheida; Moradi, Shokoufeh

doi:10.1039/d0ay01993c

Cited by 8 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A wide range of non-covalent interactions together with a flexible structure enable analyte-induced conformational matching and offer the possibility to detect a lot species such as complementary DNA/RNA (such sensors are called genosensors), as well as non-nucleic acid analytes (such sensors are called aptasensors) [ 9 , 10 ]. The range of analytes for currently known aptasensors covers low-molecular-weight chemical compounds (toxins and drugs) or antibodies and proteins exhibiting an affinity for specific nucleic acid sequences [ 11 , 12 , 13 , 14 , 15 ]. Aptasensors can be also used in environmental monitoring for heavy metal detection [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

View full text Add to dashboard Cite

To meet the requirements of novel therapies, effective treatments should be supported by diagnostic tools characterized by appropriate analytical and working parameters. These are, in particular, fast and reliable responses that are proportional to analyte concentration, with low detection limits, high selectivity, cost-efficient construction, and portability, allowing for the development of point-of-care devices. Biosensors using nucleic acids as receptors has turned out to be an effective approach for meeting the abovementioned requirements. Careful design of the receptor layers will allow them to obtain DNA biosensors that are dedicated to almost any analyte, including ions, low and high molecular weight compounds, nucleic acids, proteins, and even whole cells. The impulse for the application of carbon nanomaterials in electrochemical DNA biosensors is rooted in the possibility to further influence their analytical parameters and adjust them to the chosen analysis. Such nanomaterials enable the lowering of the detection limit, the extension of the biosensor linear response, or the increase in selectivity. This is possible thanks to their high conductivity, large surface-to-area ratio, ease of chemical modification, and introduction of other nanomaterials, such as nanoparticles, into the carbon structures. This review discusses the recent advances on the design and application of carbon nanomaterials in electrochemical DNA biosensors that are dedicated especially to modern medical diagnostics.

show abstract

Section: Introductionmentioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

View full text Add to dashboard Cite

show abstract

Evolution of nucleic acids biosensors detection limit III

et al. 2021

View full text Add to dashboard Cite

This review is an update of two previous ones focusing on the limit of detection of electrochemical nucleic acid biosensors allowing direct detection of nucleic acid target (miRNA, mRNA, DNA) after hybridization event. A classification founded on the nature of the electrochemical transduction pathway is established. It provides an overall picture of the detection limit evolution of the various sensor architectures developed during the last three decades and a critical report of recent strategies.

show abstract

Construction of a novel semiconducting polymer dots-based ratiometric fluorescence nanoprobe for DNA detection

et al. 2021

View full text Add to dashboard Cite

The development of an electrochemical DNA biosensor based on quercetin as a new electroactive indicator for DNA hybridization detection

Abstract: An electrochemical DNA biosensor was designed based on quercetin for the detection of a specific target DNA after hybridization with a complementary DNA probe immobilized onto a glassy carbon electrode surface.

Cited by 8 publications

References 31 publications

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Evolution of nucleic acids biosensors detection limit III

Construction of a novel semiconducting polymer dots-based ratiometric fluorescence nanoprobe for DNA detection

Contact Info

Product

Resources

About