The development of an in vitro 3D model of goblet cell hyperplasia using MUC5AC expression and repeated whole aerosol exposures

E-cigarette synthetic cooling agent WS-23 and nicotine aerosols differentially modulate airway epithelial cell responses

“…The present data in this study using the ALI cells also corroborate these findings where WS-23 aerosols show a trend towards increased MUC5AC expression in AECs at 72 h post-exposure. The E-cig aerosols containing nicotine, however, showed a significant increase in MUC5AC expression supporting the previous findings that nicotine exposure is a strong inducer of mucin MUC5AC expression [9] , [15] . Here, we report that WS-23 may be inducing a similar response independent of nicotine presence and may require further investigation into long-term effects.…”

E-Cigarette Synthetic Cooling Agent WS-23 and Nicotine Aerosols Differentially Modulate Airway Epithelial Cell Responses

“…(Li et al, 2005, Kaneko and Szallasi, 2014) Nicotine exposure has been well established as an inducer of mucin MUC5AC expression. (Escobar et al, 2021, Haswell et al, 2021) Here, we report that WS-23 may be inducing a similar response independent of nicotine presence and may require further investigation into long-term effects.…”

Evaluation of behavioural, chemical, toxicological and clinical studies of a tobacco heated product glo™ and the potential for bridging from a foundational dataset to new product iterations

“…The measurements show that many key pathways such as inflammation were activated and are likely to contribute to injury and disease. At doses equivalent to reference cigarette smoke, the THP Type 1, original, aerosol, TPM or AqE did not induce a significant response in any of the assays or endpoints tested [31] , [32] , [55] , [56] , [57] .…”

“…AA, TEERs and CBF were all adversely effected in response to cigarette smoke exposure, demonstrating reduce lung function. THP aerosol did not adversely affect the cells and MUC5AC over-production was not observed [31] Abbreviations: THP = tobacco heating product, WA = whole aerosol; GVP = gas vapour phase; n/a = not applicable; IVMN = in vitro micronucleus; AqE = aqueous extracts; GCH = goblet cell hyperplasia; TEER = transepithelial resistance; CBF= cilia beating frequency; AA = active area; TPM = total particulate matter 1 Bhas_42_CTA_TG_HRI_Draft_Rev_7.pdf (oecd.org). The Bhas 42 cell are established from BALB/c 3T3 cells by the transfection of v-Ha-ras gene.…”

“…As discussed, THP Type 1, original, has been investigated using a battery of in vitro mutagenicity and carcinogenicity toxicological assays conducted in accordance with GLP, following established international test guidelines. Across all assays, including the bacterial reverse mutation assay (Ames), in vitro micronucleus (IVMN), mouse lymphoma (MLA), Bhas 42 cell transformation assay and the neutral red uptake assay (NRU), results have all shown either limited activity or significant reductions in activity in tests of THP Type 1, original, emissions as compared to cigarette smoke [31] , [32] , [49] , [50] , [51] , [52] .…”