Tobacco is an important economic crop with high economic value. At present, China's tobacco planting area and total output ranks first in the world, and the tobacco industry plays an important role in China's economic development. Based on the above background, the purpose of this study was to study the effects of cake fertilizer and humic acid on soil nutrients and growth of Flue-cured Tobacco under VR vision. The effects of humic acid fertilizer and cake fertilizer on the growth, nitrogen, phosphorus, potassium content, nicotine content and chemical composition of flue-cured tobacco were studied by using randomized block test method with no fertilization as blank control. The causes of improving yield and quality of flue-cured tobacco were discussed, The results showed that humic acid and cake fertilizer improved the dry matter quality of root, stem, lower leaves and middle leaves of flue-cured tobacco, which were 22.69%, 16.52%, 27.25%, 15.44% and 41.50% higher than those of conventional fertilization, respectively. It showed that humic acid fertilizer increased the biomass of Flue-cured tobacco, provided material basis for the improvement of yield and quality, and promoted dry matter accumulation of various organs.