The development of immunoassays to four biological threat agents in a bidiffractive grating biosensor

O'Brien, Tom; Johnson, Linwood H; Aldrich, Jennifer; Allen, Steven G.; Liang, Liting; Plummer, Andrea; Krak, Stephen J.; Boiarski, Anthony A.

doi:10.1016/s0956-5663(99)00061-5

Cited by 62 publications

(14 citation statements)

References 13 publications

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“…Another approach, an optical based sensor using immunomagnetic separation coupled with electro-chemiluminescence and fluorescence, yielded a significantly reduced detection time of just 30 min (Yu et al 2000). Advances in an alternate technique, rapid chromatograph assays, have brought detection times down even lower, successfully detecting an analyte in just 15 min while also maintaining a high sensitivity (O'Brien et al 2000). Following the development of rapid chromatograph assays, flourescence array biosensensors were shown to detect multiple agents simultaneously with a response time of 14 min, and a fairly low detection limit of only 10 5 colonyforming-units (cfu)/mL (Rowe et al 1999).…”

Section: Biological Sensorsmentioning

confidence: 99%

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Detection of a surrogate biological agent with a portable surface plasmon resonance sensor onboard an unmanned aircraft system

Palframan

Gruszewski

Schmale

et al. 2014

J. Unmanned Veh. Sys.

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A system was developed to perform near real-time biological threat agent (BTA) detection with a small autonomous unmanned aircraft system (UAS). Biological sensors recently reached a level of miniaturization and sensitivity that have made UAS integration a feasible task. A surface plasmon resonance (SPR) biosensor was integrated into a small UAS platform for the first time, providing the UAS with the capability to collect and then quantify the concentration of a surrogate biological agent in near realtime. The sensor operator ran the SPR unit through a ground-station laptop, viewing the sensor data in real time during flight. An aerial sampling mechanism was also developed for use with the SPR sensor. The sampling system utilized a custom impinger setup to collect and concentrate aerosolized particles. The SPR and sampling system's feasibility was demonstrated using an aerosolized sucrose solution as a mock BTA. Three field experiments were carried out to test and validate the biological sampling system. In the first field experiment, the collection system was tested by flying the UAS through a ground-based plume of water-soluble blue dye. In the second field experiment, a sucrose solution was autonomously aerosolized, collected, and then detected by the combined sampling and SPR sensor subsystems onboard the UAS. In the third field experiment, a dye was released from one UAS (the leader) and captured by another UAS (the follower). Together, these field experiments illustrate the capability of the UAS to detect and quantify the concentration of a BTA released at altitude. Our integrated SPR system sets the stage for future work to detect and track BTAs in the atmosphere and assist in localizing their sources.Résumé : Un système a été élaboré afin de détecter des agents de menace biologique en temps quasi réel à l'aide d'un petit système d'aéronef sans pilote (UAS) autonome. Les capteurs biologiques on atteint récemment un niveau de miniaturisation et de sensibilité qui ont rendu possible leur intégration aux UAS. Pour la première fois, un biocapteur de réso-nance plasmonique de surface (SPR) a été intégré à une petite plateforme d'un UAS, lui donnant la capacité de faire la collecte puis de quantifier la concentration d'un agent biologique de substitution en temps quasi réel. L'opérateur de capteur a fait passer l'unité SPR par un ordinateur portable au sol qui permettait de visionner les données du capteur en temps réel pendant le vol. Un mécanisme d'échantillonnage aérien a également été élaboré et utilisé avec le capteur SPR. Le système d'échantillonnage utilisait une configuration d'impacteur personnalisé afin de collecter et de concentrer les particules aérosolisées. La viabilité du

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Section: Biological Sensorsmentioning

confidence: 99%

“…BTAs, which include bacteria, fungi, and viruses, remain a serious threat to both civilian and military populations (O'Brien et al 2000). A small UAS that could detect BTAs in near-real-time would have a number of potential applications.…”

Section: Introductionmentioning

confidence: 99%

Detection of a surrogate biological agent with a portable surface plasmon resonance sensor onboard an unmanned aircraft system

Palframan

Gruszewski

Schmale

et al. 2014

J. Unmanned Veh. Sys.

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“…Most of them detect BoNT in the ng/mL-range (Ogert et al 1992;Shriver-Lake et al 1993;Kumar et al 1994;O'Brien et al 2000;Varnum et al 2006;Grate et al 2009;Ren and Pearton 2012). Only few of them have been tested with complex matrices like food, clinical, or environmental samples (Ganapathy et al 2008;Weingart et al 2012).…”

Section: Rapid Detection Tools Based On Elisa Formatsmentioning

confidence: 99%

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Dorner

Schulz

Kull

et al. 2012

Current Topics in Microbiology and Immunology

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The detection of botulinum neurotoxins (BoNT) is extremely challenging due to their high toxicity and the multiple BoNT variants. To date, seven serotypes with more than 30 subtypes have been described, and even more subtypes are expected to be discovered. The fact that the BoNT molecules are released as large complexes of different size and composition adds further complexity to the issue. Currently, in the diagnostics of botulism, the mouse bioassay (MBA) is still considered as gold standard for the detection of BoNT in complex sample materials. Over the years, different functional, immunological, and spectrometric assays or combinations thereof have been developed, supplemented by DNA-based assays for the detection of the organism. In this review, advantages and limitations of the current technologies will be discussed, highlighting some of the intricacies of real sample analysis.

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“…not containing the analyte of interest) can be processed without the need for regenerating the detection chemistry or changing the sensor configuration. (2) Following analyte detection, the sensor can usually be reset by removing the target analyte with a low pH wash step [12][13][14]. (3) SPR sensors are universal detectors, since they can detect both BW and CW agents with the same instrument.…”

Section: Introductionmentioning

confidence: 99%

“…(3) SPR sensors are universal detectors, since they can detect both BW and CW agents with the same instrument. (4) Direct detection with SPR sensors does not continuously consume reagents, unlike the majority of other detection procedures, such as enzyme linked imunosorbent assays (ELISA) [15][16][17][18], light addressable potentiometric sensors (LAPS) [19][20][21], array biosensors [17,22,23], immunomagnetic separation coupled with electro-chemiluminescence and fluorescence procedures (IMS-ECL and IMS-FCL) [18,[24][25][26] or rapid chromatographic assays (RCA) [14], all of which require the additional analytes used in sandwich assays. In addition to a direct detection mode, SPR biosensors can also work in an amplification mode to further lower the detection limit.…”

Section: Introductionmentioning

confidence: 99%

Airborne analyte detection with an aircraft-adapted surface plasmon resonance sensor system

Naimushin¹,

Spinelli

Soelberg³

et al. 2005

Sensors and Actuators B: Chemical

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The development of immunoassays to four biological threat agents in a bidiffractive grating biosensor

Cited by 62 publications

References 13 publications

Detection of a surrogate biological agent with a portable surface plasmon resonance sensor onboard an unmanned aircraft system

Detection of a surrogate biological agent with a portable surface plasmon resonance sensor onboard an unmanned aircraft system

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Airborne analyte detection with an aircraft-adapted surface plasmon resonance sensor system

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