The development of multiple probe microdialysis sampling in the stomach

Woo, Kristin L.; Lunte, Craig E.

doi:10.1016/j.jpba.2008.04.019

Cited by 16 publications

(12 citation statements)

References 21 publications

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“…Alternatives to solving an adsorption problem are to test different tubing materials that may act differently and/or to add 0.25 or 0.5% albumin to the perfusate. Woo and Lunte concluded that the microdialysis behavior of some compounds improved after adding albumin, but not all, and that lipophilicity per se was not the issue, making the problem difficult to solve (57). If the measures of adding albumin or changing tubing material do not solve the problem, it is recommended not to perform microdialysis.…”

Section: In Vitro Preparations and Adsorption To Tubings And Probementioning

confidence: 99%

“…If the measures of adding albumin or changing tubing material do not solve the problem, it is recommended not to perform microdialysis. More detailed recommendation on how to address the problem with adsorption can be found in the AAPS-FDA workshop white paper (16), as well as in (18,24,57,58). However, in the paper by Nirogi et al, a 60-min pre-equilibration was used, which may hide possible adsorption (58).…”

Section: In Vitro Preparations and Adsorption To Tubings And Probementioning

confidence: 99%

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Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Hammarlund‐Udenaes

2017

AAPS J

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Abstract.Microdialysis has contributed with very important knowledge to the understanding of target-specific concentrations and their relationship to pharmacodynamic effects from a systems pharmacology perspective, aiding in the global understanding of drug effects. This review focuses on the historical development of microdialysis as a method to quantify the pharmacologically very important unbound tissue concentrations and of recent findings relating to modeling microdialysis data to extrapolate from rodents to humans, understanding distribution of drugs in different tissues and disease conditions. Quantitative microdialysis developed very rapidly during the early 1990s. Method development was in focus in the early years including development of quantitative microdialysis, to be able to estimate true extracellular concentrations. Microdialysis has significantly contributed to the understanding of active transport at the blood-brain barrier and in other organs. Examples are presented where microdialysis together with modeling has increased the knowledge on tissue distribution between species, in overweight patients and in tumors, and in metabolite contribution to drug effects. More integrated metabolomic studies are still sparse within the microdialysis field, although a great potential for tissue and disease-specific measurements is evident.

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Section: In Vitro Preparations and Adsorption To Tubings And Probementioning

confidence: 99%

Section: In Vitro Preparations and Adsorption To Tubings And Probementioning

confidence: 99%

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Hammarlund‐Udenaes

2017

AAPS J

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“…Saline was perfused through the microdialysis probe at a rate of 1 ml/min and fractions of dialysate were collected during 5-minute intervals. The microdialysis probe was equilibrated for 25 minutes and fractions were collected to determine the basal concentrations of cGMP concentrations in the submucosa (Woo and Lunte, 2008). Vehicle (D-glucose: 10 mg in H 2 O) was injected into the lumen of the colon loop and the collection of dialysate fractions was continued for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…(A) Following a 20-minute equilibration period, a sub-EC 50 concentration of linaclotide (6 nM) was added to the apical chamber for 20 minutes, followed by addition of a concentration of linaclotide inducing a maximum increase in I sc (1000 nM) for another 20-minute period. In a parallel experiment, colonic mucosa was pretreated with MK571 (20 mM) for 20 minutes prior to addition of linaclotide (6 and ( Woo and Lunte, 2008). Here, we measured for the first time in vivo linaclotide-induced cGMP efflux into the colonic submucosal layer.…”

Section: Mrp4 Modulates Gc-c-dependent Electrolyte and Cgmp Secretionmentioning

confidence: 99%

MRP4 Modulation of the Guanylate Cyclase-C/cGMP Pathway: Effects on Linaclotide-Induced Electrolyte Secretion and cGMP Efflux

Tchernychev

Kessler

et al. 2015

J Pharmacol Exp Ther

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MRP4 mediates the efflux of cGMP and cAMP and acts as an important regulator of these secondary messengers, thereby affecting signaling events mediated by cGMP and cAMP. Immunofluorescence staining showed high MRP4 expression localized predominantly in the apical membrane of rat colonic epithelium. In vitro studies were performed using a rat colonic mucosal layer mounted in an Ussing chamber. Linaclotide activation of the guanylate cyclase-C (GC-C)/cGMP pathway induced a concentration-dependent increase in transepithelial ion current [short-circuit current (I sc )] across rat colonic mucosa (EC 50 : 9.2 nM). Pretreatment of colonic mucosa with the specific MRP4 inhibitor MK571 potentiated linaclotide-induced electrolyte secretion and augmented linaclotide-stimulated intracellular cGMP accumulation. Notably, pretreatment with the phosphodiesterase 5 inhibitor sildenafil increased basal I sc , but had no amplifying effect on linaclotide-induced I sc . MRP4 inhibition selectively affected the activation phase, but not the deactivation phase, of linaclotide. In contrast, incubation with a GC-C/Fc chimera binding to linaclotide abrogated linaclotide-induced I sc , returning to baseline. Furthermore, linaclotide activation of GC-C induced cGMP secretion from the apical and basolateral membranes of colonic epithelium. MRP4 inhibition blocked cGMP efflux from the apical membrane, but not the basolateral membrane. These data reveal a novel, previously unrecognized mechanism that functionally couples GC-C-induced luminal electrolyte transport and cGMP secretion to spatially restricted, compartmentalized regulation by MRP4 at the apical membrane of intestinal epithelium. These findings have important implications for gastrointestinal disorders with symptoms associated with dysregulated fluid homeostasis, such as irritable bowel syndrome with constipation, chronic idiopathic constipation, and secretory diarrhea.

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“…Most recent published works [39] involved a RP separation using UV detection at 300, 280 and 275 nm to monitor the absorption of salicylic acid (SA), caffeine and metoprolol in the stomach following …”

Section: Detectors Coupled With Hplc Analysismentioning

confidence: 99%

Current separation and detection methods in microdialysis the drive towards sensitivity and speed

Guihen

O’Connor

2009

Electrophoresis

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This review outlines some of the analytical challenges associated with the analysis of microdialysis (MD) samples, in particular, the minute complex sample volumes that are often encountered. In MD sampling many different low-molecular-weight molecules can be collected, but the research findings are often limited by the sensitivity, specificity, and reliability of the analytical technique that is coupled to the dialysis probe. Therefore it is critical that a lot of consideration is given in selecting the most suitable analytical method including the most appropriate detector. This review aims to highlight the strengths and weaknesses of a range of commonly used analytical methods employed in MD. In Section 1, a brief overview of the MD technique is described, followed by a discussion on some of the advantages and drawbacks of this sampling technique. Sections 2 and 3 examine analytical and other technical considerations regarding analysis, with special emphasis on the factors that specifically influence analytical detection. Section 4 outlines the most commonly employed analytical techniques used in MD, including HPLC coupled with various detectors. Detail is given regarding the LOD and LOQ for many applications using each detector. As MS is of such high importance in MD, a special sub-section has been devoted to it. The importance of CE is also highlighted, with specific applications described. In addition, analytical techniques that do not appear to have found routine use in MD are discussed. Section 5 is concerned with recent innovations in chemical separation techniques, in particular MCE and ultra-performance liquid chromatography. Specific applications of the coupling of these techniques with MD are highlighted, along with technical challenges associated with miniaturization. In the Section 6, the future outlook of MD is discussed. Techniques other than electrophoretic- and chromatographic based separation methods are outside the scope of this review.

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The development of multiple probe microdialysis sampling in the stomach

Cited by 16 publications

References 21 publications

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

Microdialysis as an Important Technique in Systems Pharmacology—a Historical and Methodological Review

MRP4 Modulation of the Guanylate Cyclase-C/cGMP Pathway: Effects on Linaclotide-Induced Electrolyte Secretion and cGMP Efflux

Current separation and detection methods in microdialysis the drive towards sensitivity and speed

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