The Development of Multiplex PCR Microsatellite Marker Sets for Korean Chicken Breeds

Kong, Hong Sik; Choi, Sung Soo; Seo, Joo Hee; Han, Jung-Min; Seong, Jiyeon; Lee, Jun Heon

doi:10.3923/ijps.2019.492.498

Cited by 3 publications

(5 citation statements)

References 11 publications

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“…Moreover, the total number of alleles per strain agreed with Kayang et al [ 15 ], who reported that the average of 1.9 alleles per locus ranged from one to four alleles. Also, Choi et al [ 39 ] reported that the mean number of alleles in each breed ranged from 3.59 to 6.63. Further studies carried on different quail genotypes by Bai et al [ 40 ], Kawahara-Miki et al [ 37 ], Bai et al [ 38 ], Shimma and Tadano [ 41 ], and Habimana et al [ 10 ] reported the allele size of 48, 70, 197, 308, and 305, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genetic and physiological variation in two strains of Japanese quail

Ibrahim

El-Sayed

Assi

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Detecting the genetic and physiological variations in two Japanese quail strains could be used to suggest a new avian model for future breeding studies. Consequently, two estimations were performed on two Japanese quail strains: gray quail strain (GJQS) and white jumbo quail strain (WJQS). The first estimation was conducted on carcass characteristics, breast muscles, breast concentration of collagen type I, and body measurements. In contrast, blood samples were collected for the second estimation for genomic DNA extraction and genetic analysis. Results A total of 62 alleles out of 97 specific alleles (63.92%) were detected overall loci (14 microsatellite loci) for the two strains. A total of 27 specific alleles of WJQS were observed, and 35 were obtained for GJQS. The percentage of similarity was 48.09% ranged from 4.35 with UBC001 to 100% with GUJ0051. WJQS had greater body weights and a higher value of pectoral muscle and supracoracoideus muscle than GJQS. The breast muscles of GJQS exhibited a higher concentration of type I collagen than the WJQS. Furthermore, males showed higher concentrations of collagen type I than females. WJQS showed a higher body length, chest girth, chest length, thigh length, thigh girth, drumstick length, and drumstick girth (cm) than GJQS. WJQS showed more significant differences in carcass traits compared with GJQS. Conclusion The physiological differences between WJQS and GJQS were ascertained with microsatellite markers, which indicated high polymorphism between these strains. These observations provided a scientific basis for evaluating and utilizing the genetic resources of WJQS and GJQS in a future genetic improvement program.

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Section: Discussionmentioning

confidence: 99%

Genetic and physiological variation in two strains of Japanese quail

Ibrahim

El-Sayed

Assi

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…Previously, 27 Microsatellite markers were investigated for the discrimination of KNC and commercial KNC (Seo et al, 2015;Seo et al, 2017;Choi et al, 2019). From these results, a total of 12 MS markers were initially selected, which have high expected heterozygosity (H Exp ) and polymorphic information content (PIC) values (Supplementary Table 1).…”

Section: Microsatellite (Ms) Marker and Polymerase Chain Reaction (Pcr) Amplificationmentioning

confidence: 99%

“…NIAS has preserved two types of purebred chicken breeds: purebred KNCs, which include five breeds with different feather colors {red-brown (NR), yellow-brown (NY), gray-brown (NG), black (NL) and white (NW)} and the "imported and adapted chickens", which includes two Rhode Island Red breeds, two Cornish breeds and two Leghorn breeds (Seo et al, 2018;Choi et al, 2019). Also, NIAS developed the Woorimatdag version 1 (WM1) and 2 (WMT).…”

mentioning

confidence: 99%

“…WM1 breeds were commercial, KNC breeds generated form crossbreeding fast growing native male and good tasting female with increased egg production, and WM2 breeds were modified version of WM1 breeds with increased growth rates (Park et al, 2010;Choi et al, 2015). The private native chicken breeding-stock company (Hanhyup) is responsible for more than 80% of the native chicken distribution in Korea and has maintained purebred chicken breeds (Hanhyup breeds) for commercial use for the past 60 years (Seo et al, 2018;Choi et al, 2019). Hanhyup breeds produced by mating the KNC and economically superior and naturalized breeds (Seo et al, 2017).…”

mentioning

confidence: 99%

“…PIC values ranged from 0.445 (MCW0123) to 0.691 (MCW0127), with a mean value of 0.610. Estimation of genotypic diversity in heterozygosity and PIC value of MS markers were previously used for determining animal breed selection(Berthouly et al, 2008;Choi et al, 2019).…”

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confidence: 99%

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Genetic diversity and relationship of Korean chicken breeds using 12 microsatellite markers

Kim

Yun

Moon

et al. 2021

J Anim Reprod Biotechnol

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Chicken is one of the major livestock, especially for supplying proteins to human and the Korean native chicken (KNC) has been documented since approximately 2,000 years ago (Seo et al., 2013;Seo et al., 2015). But, due to their poor commercial performance, Korean native chicken breeds almost became extinct and the breeds that existed before the Korean War (1950)(1951)(1952)(1953), are almost all extinct (Seo et al., 2013;Choi et al., 2015). After the Korean War, commercial native chicken companies maintained various independent breeds while continuing production and market distribution (Seo et al., 2018).

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Allelic polymorphism of microsatelite DNA locі of egg chikens

Shelyov¹,

Kopylov²,

Prokopenko³

et al. 2020

Sučasne ptahìvnictvo

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The analysis of allelic polymorphism of five industrial egg crosses of chickens by five microsatellite DNA loci (ADL0268, MCW216, LEI0094, ADL0278, and MCW248) was carried out. DNA loci were chosen according to the recommendations of the International Society for Animal Genetics (ISAG). Based on the results of mathematical-statistical processing and data analysis, the spectra and frequencies of allelic variability, the peculiarities of allele pools, were identified, and unique alleles were identified. In general, the species Gallus gallus is characterized by a specific character of allelic spectra for all investigated microsatellite DNA loci (P <0.001). The highest rates of allelic variability were recorded in brown crosses "Lohmann brown" and "Hisex brown" (Na (LimNa)=(9.2 (5-17) and 7.4 (6-11), respectively). The studied crosses were characterized by a shift in allelic spectra towards a decrease in the fragment length. “Lohmann white” stands out among the birds of other crosses by high consolidation for individual alleles for all studied microsatellites (from ADL278114 – 0.343 and ADL268108 – 0.485 to LEI094259 – 0.720, MCW0248213 – 0.785 and MCW0216137 – 0.920). Unique alleles with the highest frequency were found in brown cross chickens, and in the “Hy-Line W-98” bird, they were not found. The number of unique alleles identified varied from 1 ("Hisex white") to 11 ("Lohmann brown"). Locus LEI094 turned out to be the most polymorphic in terms of the number of unique alleles – 10 such allelic variants were identified for it. No unique alleles were identified at the ADL0268 locus. The obtained estimates criterion χ2 of K. Pearson indicate significant differences in the frequency distribution of alleles for all studied loci. When using the MICROSATELLITE ANALYSER software, it was found that the nature of the variability of the studied microsatellite DNA loci in five industrial crosses of the egg chickens, both in the number of identified alleles and in the nature of their distribution, corresponded to the stepwise mutation model (SMM).

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The Development of Multiplex PCR Microsatellite Marker Sets for Korean Chicken Breeds

Cited by 3 publications

References 11 publications

Genetic and physiological variation in two strains of Japanese quail

Genetic and physiological variation in two strains of Japanese quail

Genetic diversity and relationship of Korean chicken breeds using 12 microsatellite markers

Allelic polymorphism of microsatelite DNA locі of egg chikens

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