The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties

Abstract

To assess the potential of multiplex SSR markers for testing distinctness, uniformity and stability of rape (Brassica napus L.) varieties, we developed three multiplex SSR sets composed of five markers each. These were used to measure the extent of diversity within and between a set of ten varieties using a fluorescence-based semi-automated detection technology. Also, we evaluated the significance of any correlation between SSRs, pedigree and five of the morphological characters currently used for statutory distinctness, uniformity and stability testing of rape varieties. An assignment test was allowed to identify 99% of the plants examined, with the correct variety based on the analysis of 48 individual plants for each variety. Principal coordinate analysis confirmed that a high degree of separation between varieties could be achieved. Varieties were separated in three groups corresponding to winter, spring and forage types. These results suggested that it should be possible to select a set of markers for obtaining a suitable separation. Diversity within varieties varied considerably, according to the variety and the locus examined. No significant correlation was found between SSR and morphological data. However, genetic distances measured by SSRs were correlated to pedigree. These results suggested that SSRs could be used for pre-screening or grouping of existing and candidate varieties, allowing the number of varieties that need to be grown for comparison to be reduced. Multiplex SSR sets gave high-throughput reproducible results, thus reducing the costs of SSR assessment. Multiplex SSR sets are a promising way forward for complementing the current variety testing system in B. napus.

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

“…There are a variety of DNA marker techniques, such as random amplified polymorphic sequence (RAPD) (Juchum et al, 2007;Ro et al, 2007), amplified restriction fragment polymorphism (AFLP) (Percifield, 2007;Yuan et al, 2007) and microsatellite markers (SSR) (Legesse et al, 2007;Tommasini et al, 2003). They are widely applied in species or varieties identification, evaluation of genetic diversity, but few reports are available on systematically comparative analysis of two kinds of testing approaches, such as morphological method and molecular marker method.…”

Analysis of Genetic Polymorphism of Korean Ginseng Cultivars and Foreign Accessions using SSR Markers

“…그러나, 인삼의 경우 외부 형태적 특성에 의한 유전자원의 평가는 유 전자형과 환경 간의 상호작용과 해가림 시설 등 재배방법에 따라서 표현형이 달라지기 때문에 적당하지 않다. 이러한 문 제점을 보완하기 위하여, 환경에 영향을 받지 않는 DNA 마 커를 이용한 유전적 다양성 분석과 품종 판별 에 관한 연구가 진행되어, 오이 (Bernet et al, 2003), 대청 , 유채 (Tommasini et al, 2003), 고추 (Kwon et al, 2005), 밀 (Noil et al, 2008), 사과 (Cho et al, 2010 (Wu and Tanksley, 1993;Saghai-Maroof et al, 1994;Morgante et al, 1995;Ogbonnaya et al, 2008).…”

The disease reactions of heirloom bell pepper “California Wonder” to <i>Phytophthora capsici</i>

“…Genotype diversity is a measure of uniformity of individuals within a cultivar/accession, and ranges from 0 to 1 with zero indicating no variation within the cultivar/accession [21].…”

“…Powermarker was also used to calculate heterozygosity [19] and polymorphism information content (PIC) [20], while genotype diversity was calculated as described by Tommasini et al [21].…”