The Development of Newborn C 57 BL/KsJ-dbm Mice Produced from Eggs Fertilized in Vitro

Suzuki, Hiroshi; Shingai, Saburo; Togashi, Mamoru; Miyai, Tatsuya; Motohide, Yukage; Okamoto, Michio

doi:10.1538/expanim1978.37.3_245

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…In vitro fertilization (IVF) has been used frequently for the treatment of sterility in humans; to improve the quality and increase production in cattle (Suzuki et al, 1996). In laboratory animals, IVF has been used for analysis of fertilization events and to ameliorate defects in the reproductive ability of mutant mice (Suzuki et al, 1987(Suzuki et al, , 1988(Suzuki et al, , 1996Yokoyama et al, 1995) or aged mice (Suzuki et al, 1994). Early studies have shown that the addition of E 2 to oocyte maturation medium could increase the fertilization and cleavage rates of the in vitro matured human oocytes (Tesarik & Mendoza 1995).…”

Section: Introductionmentioning

confidence: 99%

Full term development of normal mice after transfer of IVF embryos derived from oocytes stored at room temperature for 1 day

Lei

Huang

Shi

et al. 2008

Zygote

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Early studies have shown that some mouse cumulus-oocyte complexes (COCs) stored at room temperature for 24 h still retained full developmental potential. In this study, we stored denuded mouse oocytes (DOs) at room temperature (25 degrees C) for 24 h and activated these oocytes with 10 mM SrCl2 or fertilized the oocytes by IVF. We found that nearly half of the DOs stored at room temperature for 1 day can be fertilized normally by IVF and that two foster mothers gave birth to seven pups. Embryos from stored oocytes were cultured in CZB medium with or without 1 microg/ml 17beta-estradiol (E2). The numbers of embryo that developed to morula/blastocyst stage after parthenogenetic activation and IVF were significantly increased when E2 was added to the culture (p<0.05). These results suggest that E2 might improve mouse embryo development in vitro. The birth of seven agouti pups and their healthy growth indicated that the storage of DOs at room temperature for 1 day may be a practical procedure for mammalian reproduction.

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Section: Introductionmentioning

confidence: 99%

Full term development of normal mice after transfer of IVF embryos derived from oocytes stored at room temperature for 1 day

Lei

Huang

Shi

et al. 2008

Zygote

View full text Add to dashboard Cite

show abstract

Rederivation of Mice by Means of In Vitro Fertilization and Embryo Transfer.

et al. 1996

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In vitro fertilization and embryo transfer were performed for rederivation of four strains of mice harbouring mouse hepatitis virus (MHV) and/or Pasteurella pneumotropica (P. pneumotropica). Superovulated oocytes were fertilized by preincubated cauda epididymis sperm in vitro. Fertilized eggs at 2-cell stage were transferred into the oviducts of specific pathogen free (SPF) recipients. Microbial examination of sperm and/or oocyte donors verified the presence of P. pneumotropica and/or of antibodies to MHV in all strains, but neither in the recipients nor in the offspring antibodies to MHV could they be detected. The results indicate that an in vitro fertilization-embryo transfer (IVF-ET) system is an effective and simple alternative to cesarean operation in infected mice.

show abstract

The Development of Newborn C 57 BL/KsJ-dbm Mice Produced from Eggs Fertilized in Vitro

Cited by 2 publications

References 6 publications

Full term development of normal mice after transfer of IVF embryos derived from oocytes stored at room temperature for 1 day

Full term development of normal mice after transfer of IVF embryos derived from oocytes stored at room temperature for 1 day

Rederivation of Mice by Means of In Vitro Fertilization and Embryo Transfer.

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