The development of oocyte cryopreservation techniques in blue mussels Mytilus galloprovincialis

Wang, Hanru; Li, Xiaoxu; Wang, Meiqing; Clarke, Steven; Gluis, Mark

doi:10.1007/s12562-014-0796-9

Cited by 12 publications

(16 citation statements)

References 35 publications

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“…) and the mussels Mytilus galloprovincialis Lamarck (Wang et al . ) and Pe. canaliculus (Adams et al .…”

Section: Marine Invertebratesmentioning

confidence: 95%

“…In a similar manner, in a study by Wang et al . (), although the CPT mixture of EG and trehalose yielded satisfactory results for surviving oocytes (fertilisation rate of 32%), few embryos developed into D‐larvae.…”

Section: Marine Invertebratesmentioning

confidence: 98%

“…), mussel oocytes (Wang et al . ), sea urchin embryos (Bellas & Paredes ; Odintsova et al . ) and starfish oocytes (Hamaratoglu et al .…”

Section: Marine Invertebratesmentioning

confidence: 99%

“…No specific pattern has been followed for cryopreserving any particular cell type of marine invertebrates using glucose, sucrose and trehalose. Nevertheless, trehalose has been used as a CPT supplement for a vast range of cells of invertebrates, including oyster larvae and sperm (Adams et al 2004;Suquet et al 2014), mussel oocytes (Wang et al 2014), sea urchin embryos (Bellas & Paredes 2011;Odintsova et al 2015) and starfish oocytes (Hamaratoglu et al 2005). Sucrose has been used for cryopreserving oyster larvae and embryos (Renard 1991;Choi & Chang 2003Kim et al 2013).…”

Section: Marine Invertebratesmentioning

confidence: 99%

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Sugars as supplemental cryoprotectants for marine organisms

Tsai

Chong

Meng

et al. 2017

Reviews in Aquaculture

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Cryopreservation has been extensively used in various mega‐industries and has recently been applied in genetic banking for conservation purposes. Compared with conventional cell preservation methods, cryopreservation can maintain the viability of cryopreserved cells for an indefinite time at a relatively lower cost and lesser manpower, with lower probabilities of contamination and genetic changes. This study presents the crucial role of sugar, a cryoprotectant supplement in cryopreservation. Sugar molecules typically interact with the lipid bilayer during the freezing phase to maintain plasma membrane integrity when cells undergo dehydration. When combined with other permeable cryoprotectants such as glucose with methanol and trehalose with dimethyl sulphoxide, sugar prolongs and enhances cellular post‐thaw viability. Moreover, terrestrial and marine organisms have benefited from the inclusion of sugar in the cryopreservation protocol. For wide range of cells such as the sperm, oocytes, embryos and larvae of marine vertebrates and invertebrates, as well as marine algae, cryopreservation with sugar produced positive results compared with cryopreservation without sugar. Not all sugar is beneficial, and the type and concentration of sugar should be applied according to the species. Moreover, the freezing method may also affect the function of sugar. Nevertheless, understanding the role of sugar in cryobiology and conducting a preliminary trial of sugar for cryopreserving cells would benefit future research on cryopreservation.

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“…) and the mussels Mytilus galloprovincialis Lamarck (Wang et al . ) and Pe. canaliculus (Adams et al .…”

Section: Marine Invertebratesmentioning

confidence: 95%

Section: Marine Invertebratesmentioning

confidence: 98%

“…), mussel oocytes (Wang et al . ), sea urchin embryos (Bellas & Paredes ; Odintsova et al . ) and starfish oocytes (Hamaratoglu et al .…”

Section: Marine Invertebratesmentioning

confidence: 99%

Section: Marine Invertebratesmentioning

confidence: 99%

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Sugars as supplemental cryoprotectants for marine organisms

Tsai

Chong

Meng

et al. 2017

Reviews in Aquaculture

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“…While there have been many successes in this field for some species and/or certain types of biological material, a number of challenges remain. For example, cryopreservation of fish and shellfish sperm has been achieved; however, cryopreservation of oocytes is still highly problematic (Herr aez et al 2011;Wang et al 2014;Hassan et al 2015). In molluscs, cryopreservation of fertilized embryos can be accomplished, but survival rates are often extremely low Paredes et al 2012) and the reasons for poor success are unknown.…”

Section: Future Applications and Directionsmentioning

confidence: 99%

Showcasing metabolomic applications in aquaculture: a review

Young

Alfaro

2016

Reviews in Aquaculture

132

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Aquaculture production is currently challenged to meet the growing demands for seafood protein throughout the world. To achieve this growth in an efficient, safe and sustainable manner, novel tools and applications will need to be incorporated at each step of the production line. A variety of 'omics' (e.g., transcriptomics, proteomics, metabolomics) applications have already begun to emerge in aquaculture research with extreme success. A promising new 'omics' approach is metabolomics, which aims to use metabolite profiles to identify biomarkers indicative of physiological responses of living samples (e.g., whole organism, tissues, cells) to environmental or culture conditions. One of the benefits of this approach is that it uses a broad scan of biological conditions to identify often unexpected problem or risk areas to focus management attention. In this contribution, we have selected relevant research examples to showcase the applications of metabolomics in aquaculture in four major areas: hatchery production, nutrition and diet, disease and immunology, and food safety and quality. The novelty of this approach is highlighted by the fact that we have cited the majority of published papers in this field, and these are all recent (within the last decade) contributions.

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Mussel Culture

2021

Marine Mussels

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The development of oocyte cryopreservation techniques in blue mussels Mytilus galloprovincialis

Cited by 12 publications

References 35 publications

Sugars as supplemental cryoprotectants for marine organisms

Sugars as supplemental cryoprotectants for marine organisms

Showcasing metabolomic applications in aquaculture: a review

Mussel Culture

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