The Development of Piezo-Driven Tools for Cellular Piercing

Abstract

Conventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill's lateral vibration, the damping effect is not as satisfactory as mercury. In this work, a modified piezo-drill without using mercury was developed for the cellular piercing of mouse oocytes. Experimentally, appropriate parameters of driving voltage and frequency were obtained for the penetration of the zona pellucida of mouse oocytes. Furthermore, the lateral vibration of the injection pipette is lower than 1 µm in deionized water, which is not observable at 400 magnificence. With the piezo-drill without using mercury, the system performs the cellular piercing of mouse oocytes with a maximum cleavage rate of 94.7% (n = 117).

A Review of Single‐Cell Pose Adjustment and Puncture

“…The former can be toxic and pollute cells, whereas the latter is not desirable for damping, so the researchers have proposed to modify the structure of the piezoelectric injection needle. To solve the above problem, Ru et al [184] proposed two improvement methods ) Schematic of mechanical arm-driven microneedle injection of drosophila larvae at specific sites. [179] d) Microfluidic device for high-speed microinjection of C. elegans.…”

Dynamic Non-Destructive Evaluation of Piezoelectric Materials to Verify on Accuracy of Transversely Isotropic Material Property Measured by Resonance Method

“…Piezoelectric materials have superior electromechanical properties, are lightweight and easy and inexpensive to produce, and have low power consumption [1][2][3][4]. For instance, the puncture systems used in cellular biology research have precise and fine vibration control [5]. In addition to applying the vibration characteristics of inverse piezoelectricity, the puncture systems also apply energy extraction systems with direct piezoelectric effects.…”

A sensorless angular displacement measurement method for rotational oscillation generation in biomedical applications with Ros-Drill©

“…The microinjection technique is very popular and widely used in biological studies such as intracytoplasmic sperm injection (ICSI) (Ergenc, 2007; Kimura and Yanagimachi, 1995; Lu et al, 2011; Rubino et al, 2015; Yoshida and Perry, 2007), pro-nuclei DNA injection (Chow et al, 2016; Evans, 2006; Rieckher and Tavernarakis, 2017), production of transgenic animals (Hammer et al, 1985; Ittner and Götz, 2007; Kawase et al, 2001; Rieckher and Tavernarakis, 2017), cloning of organisms (Campbell et al, 1996; Onishi et al, 2000), drosophila (Brust-Mascher and Scholey, 2009; Spradling and Rubin, 1982) and worm microinjection (Evans, 2006; Mello et al, 1991; Rieckher et al, 2009) research. Since its development by the American bacteriologist Marshall A. Barber in 1911 (Barber, 1911), dramatic modifications have been made to microinjection instrumentation to increase the efficiency, application areas and usage rate for different needs (Ge et al, 2014; Huang et al, 2011; Johnson et al, 2018; Permana et al, 2016; Ru et al, 2016; Tseng and Santra, 2016; Yoshida and Perry, 2007). Among these methods, a device known as Ros-Drill © (Ergenc and Olgac, 2007; Nak and Ergenc, 2019) stands out as one of the existing techniques that uses rotationally oscillating movements to pierce the cells rather than utilizing piezo-assisted devices, which create linear motion.…”

“…Since its development by the American bacteriologist Marshall A. Barber in 1911(Barber, 1911, dramatic modifications have been made to microinjection instrumentation to increase the efficiency, application areas and usage rate for different needs (Ge et al, 2014;Huang et al, 2011;Johnson et al, 2018;Permana et al, 2016;Ru et al, 2016;Tseng and Santra, 2016;Yoshida and Perry, 2007). Among these methods, a device known as Ros-Drill Ó (Ergenc and Olgac, 2007;Nak and Ergenc, 2019) stands out as one of the existing techniques that uses rotationally oscillating movements to pierce the cells rather than utilizing piezo-assisted devices, which create linear motion.…”