The development of precise genetic stocks in two wheat cultivars and their use in genetic analysis

Abstract

The results of genetic studies of common wheat that have been conducted in Novosibirsk, Russia, over the past 20 years by a research team are summarized. The research strategy was to develop a collection of aneuploids and substitution lines to be further used for chromosomal localization of genes and in the study of the genetic variability of wheat. On the basis of two cultivars, namely Saratovskaya 29 and Diamant, we have developed 6 sets of aneuploids with a complete set of monosomic lines for each, plus sets of lines ditelosomic and monotelosomic for "standard" arms. Exploiting the monotelosomics, 108 single chromosome intervarietal substitutions, 13 lines with alien substitutions (mono-and disomics) and 11 addition lines have been developed. A collection of lines isogenic for dominant marker genes of morphological characters has also been developed. The genetic collection was used in chromosomal localization of 15 genes, for many of which chromosome arms have been determined. Positively or negatively, the question of allelism within some loci has been answered.

The inheritance and chromosomal location of morphological traits in wild wheat, Triticum turgidum L. ssp. dicoccoides

“…A gene for hairy (pubescent) auricle designated Pa, was located on chromosome 4BS (Arbuzova et al 1996). The present results agree with the report of Levy and Feldman (1989a), suggesting that a group 5 chromosome in T. dicoccoides carries a gene for hairy auricles.…”

“…This agrees with previous literature reporting that culm colour was controlled by allele Pc1 on chromosome 7B. Gene Pc2, presumably a homoeoalle, was located on chromosome 7DS (Arbuzova et al 1996). Worzella (1942) found a complete association between purple culms and coloured coleoptile in certain crosses.…”

Evolutionary History of Triticum petropavlovskyi Udacz. et Migusch. Inferred from the Sequences of the 3-Phosphoglycerate Kinase Gene

“…According to Akond and Watanabe [24], T. petropavlovskyi is more closely related to T. polonicum than to T. durum or T. turgidum . However, Arbuzova et al [60] and Efremova et al [18] report that the genes supporting the elongated glumes in T. polonicum and T. petropavlovskyi are not allelic.…”

The Inheritance of Morphological and Biochemical Traits Introgressed into Common Wheat (Triticum aestivum L.) from Aegilops speltoides Tausch

“…Monosomic analysis using monosomic lines of 'S29' and 'Dm2' developed in the Institute of Cytology and Genetics SB RAS (Arbuzova et al 1996) was used for the determination of the chromosomes carrying the introgressed genes for the traits leaf hairiness and glaucousness. Analysis of gliadin component composition was carried as described earlier (Pshenichnikova and Maystrenko 1995).…”