The development of retinal ganglion cell dendritic stratification in ferrets

Bodnarenko, Stefan R.; Yeung, Glendy; Thomas, Lia A.; McCarthy, Marie

doi:10.1097/00001756-199909290-00015

Cited by 42 publications

(47 citation statements)

References 21 publications

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“…It should be noted that we report a higher proportion of multistratified ganglion cells than previous studies on the developing ferret (Bodnarenko et al, 1999) and mouse (Tagawa et al, 1999;Bansal et al, 2000). Most likely, this reflects differences among laboratories as to the criteria used to identify a cell as multistratified.…”

Section: Discussioncontrasting

confidence: 71%

Unique Functional Properties of On and Off Pathways in the Developing Mammalian Retina

2001

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In the mature retina, the dendrites of On and Off ganglion cells are segregated into separate sublaminas of the inner plexiform layer, but early in development these processes are multistratified, ramifying more widely within this synaptic layer. The dendritic pattern exhibited by immature ganglion cells suggests that there may be a functional convergence of On and Off pathways in the developing retina, but previous studies have provided evidence against this. Here we demonstrate by patchclamp recordings and dye filling that ganglion cells with multistratified dendrites respond to the onset, as well as the offset, of light. We further show that, in the dark-adapted retina, the glutamate analog 2-amino-4-phosphonobutric acid abolishes On and Off discharges in ganglion cells with multistratified dendrites. In contrast, in cells with stratified dendrites, this drug selectively blocks On responses. These findings provide evidence for unique functional attributes of On and Off pathways in the developing retina. The properties of immature ganglion cells documented here have important implications for the roles ascribed to neuronal activity in refining connections during the early development of the visual system.

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Section: Discussioncontrasting

confidence: 71%

Unique Functional Properties of On and Off Pathways in the Developing Mammalian Retina

2001

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“…Unstratified RGC s have both ON-and OFF-center responses (Wang et al, 2001). It should be noted that before the third postnatal week in ferrets, photoreceptors are not yet mature, and thus our classification of ON and OFF cells for the early age group (P7-P10) is based entirely on morphological criteria (Wong and Oakley, 1996;Bodnarenko et al, 1999). …”

Section: Morphological Identification Of On and Off Rgcsmentioning

confidence: 99%

Mechanisms Underlying Developmental Changes in the Firing Patterns of ON and OFF Retinal Ganglion Cells during Refinement of their Central Projections

Myhr¹,

Lukasiewicz

Wong³

2001

J. Neurosci.

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Patterned neuronal activity is implicated in the refinement of connectivity during development. Calcium-imaging studies of the immature ferret visual system demonstrated previously that functionally separate ON and OFF retinal ganglion cells (RGCs) develop distinct temporal patterns of spontaneous activity as their axonal projections undergo refinement. OFF RGCs become spontaneously more active compared with ON cells, resulting in a decrease in synchronous activity between these cell types. This change in ON and OFF activity patterns is suitable for driving the activity-dependent refinement of their axonal projections. Here, we used whole-cell and perforatedpatch recording techniques to elucidate the mechanisms that underlie the developmental alteration in the ON and OFF RGC activity patterns. First, we show that before the refinement period, ON and OFF RGCs have similar spike patterns; however, during the period of segregation, OFF RGCs demonstrate significantly higher spike rates relative to ON cells. With increasing age, OFF cells require less depolarization to reach their action potential threshold and fire more spikes in response to current injection compared with ON cells. In addition, spontaneous postsynaptic currents and potentials are greater in magnitude in OFF cells than ON cells. In contrast, before axonal refinement, there are no differences in the intrinsic excitability or synaptic drive onto ON and OFF cells. Together, our results show that developmental changes in ON and OFF RGC excitability and in the strength of their synaptic drives act together to reshape the spike patterns of these cells in a manner appropriate for the refinement of their connectivity.

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“…ON-OFF RGCs respond to light onset and offset and have bilaminated dendrites arborizing in both sublaminas a and b of the IPL (Amthor et al, 1984). Early in postnatal development, the retina is populated with a preponderance of RGCs having bilaminated dendritic arbors (Maslim and Stone, 1988;Bodnarenko et al, 1999;Tian and Copenhagen, 2003;Diao et al, 2004). In mouse, the population of bilaminated RGCs is reduced significantly 2 weeks after eye opening.…”

Section: Introductionmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor and TrkB Modulate Visual Experience-Dependent Refinement of Neuronal Pathways in Retina

et al. 2007

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Sensory experience refines neuronal structure and functionality. The visual system has proved to be a productive model system to study this plasticity. In the neonatal retina, the dendritic arbors of a large proportion of ganglion cells are diffuse in the inner plexiform layer. With maturation, many of these arbors become monolaminated. Visual deprivation suppresses this remodeling. Little is known of the molecular mechanisms controlling maturational and experience-dependent refinement. Here, we tested the hypothesis that brainderived neurotrophic factor (BDNF), which is known to regulate dendritic branching and synaptic function in the brain, modulates the developmental and visual experience-dependent refinement of retinal ganglion cells. We used a transgenic mouse line, in which a small number of ganglion cells were labeled with yellow fluorescence protein, to delineate their dendritic structure in vivo. We found that transgenic overexpression of BDNF accelerated the laminar refinement of ganglion cell dendrites, whereas decreased TrkB expression or retina-specific deletion of TrkB, the cognate receptor for BDNF, retarded it. BDNF-TrkB signaling regulated the maturational formation of new branches in ON but not the bilaminated ON-OFF ganglion cells. Furthermore, BDNF overexpression overrides the requirement for visual inputs to stimulate laminar refinement and dendritic branching of ganglion cells. These experiments reveal a previously unrecognized action of BDNF and TrkB in controlling cell-specific, experience-dependent remodeling of neuronal structures in the visual system.

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The development of retinal ganglion cell dendritic stratification in ferrets

Cited by 42 publications

References 21 publications

Unique Functional Properties of On and Off Pathways in the Developing Mammalian Retina

Unique Functional Properties of On and Off Pathways in the Developing Mammalian Retina

Mechanisms Underlying Developmental Changes in the Firing Patterns of ON and OFF Retinal Ganglion Cells during Refinement of their Central Projections

Brain-Derived Neurotrophic Factor and TrkB Modulate Visual Experience-Dependent Refinement of Neuronal Pathways in Retina

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