The Development of Silica Hydride Stationary Phases for High Performance Liquid Chromatography from Conception to Commercialization

Abstract

The development of a stationary phase material for high performance liquid chromatography based on a surface of silica hydride as opposed to silanols on ordinary silica is discussed including synthetic approaches, characterization and applications. There are several synthetic approaches available to create a silica hydride surface. Modification of the Si-H moiety on the silica surface can be accomplished through use of a hydrosilation reaction. Both the intermediate silica hydride and the material modified with an organic moiety can be characterized by a number of spectroscopic as well as a variety of other methods. Further insights into the retention mechanism are provide through chromatographic measurements. The ultimate utility of any chromatographic stationary phase material is determined by its success for solving challenging analytical problems. A broad range of applications are reviewed to illustrate the versatility and usefulness of silica hydride-based stationary phases.

The utility of silica hydride‐based stationary phases for dual‐mode ultra high performance liquid chromatography separation of synthetic cathinone positional isomers

“…Operation of a column under two modes using the same solvent reservoir has been coined as "flipflop" chromatography [25]. ANP yields elution patterns similar to HILIC, but without the retention of a water layer, which results in shorter equilibration times [26][27]. Both modes for certain SiH phases were employed for synthetic cathinones, with inadequate retention and poor separations obtained using ANP [21].…”