The development of the periodontium — a largely ectomesenchymally derived unit

Cate, A. R. Ten

doi:10.1111/j.1600-0757.1997.tb00093.x

Cited by 130 publications

(62 citation statements)

References 34 publications

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“…The dental follicle is an ectomesenchymal derived structure surrounding the developing tooth germ prior to eruption [26,27]. The contained cells, which have been used in the experiments shown here, have already been described to differentiate into the osteogenic, periodontial, cementoblastic, chondrocytic and adipocytic direction [16,28,3,29].…”

Section: Discussionmentioning

confidence: 97%

Human dental follicle precursor cells of wisdom teeth: isolation and differentiation towards osteoblasts for implants with and without scaffolds

Haddouti

Skroch

Zippel

et al. 2009

Materialwissenschaft Werkst

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The human dental follicle is a developmental precursor for essential periodontal tissues such as periodontal ligament and root development. These cells can be expected to differentiate into several lineages, since they are derived from mesoderm. Especially the differentiation towards the osteogenic lineage could be interesting for tissue regeneration with or without growing on scaffold biomaterials in autologous transplantation for reconstruction of large bone defects and incorporation of teeth implants.Here we demonstrate a fast and efficient method to isolate stem cells out of the dental follicle of wisdom teeth and their more determined lineage specific commitment into the osteogenic direction.Typical markers confirmed the stem cell character of the isolated and differentiated cells and the successful differentiation has been verified in addition after lineage specific induction using corresponding stainings. In order to evaluate the quality of the cells microbiological investigations were performed and showed that all samples contained microbial species. Pre-treatment of patients with antibiotics reduced the number of microorganisms to a minimum but did not suffice to eliminate all bacteria. The predominantly found species were gram-positive cocci being either catalase-positive and oxidase-negative or catalase-and oxidase-negative. Most microorganisms belonged to the families of Streptococcaceae and Staphylococcaceae. During cultivation of the stem cells, the contamination with microorganisms could be easily suppressed by usage of standard cell culture conditions with penicillin and streptomycin.Key words: stem cell, dental follicle, osteogenic lineage, biomaterials, differentiation

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Section: Discussionmentioning

confidence: 97%

Human dental follicle precursor cells of wisdom teeth: isolation and differentiation towards osteoblasts for implants with and without scaffolds

Haddouti

Skroch

Zippel

et al. 2009

Materialwissenschaft Werkst

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“…Both Ten Cate and Korbendau suggested that supracrestal connective tissue attachment is configured early during tooth formation, and that these collagen fibers attached to the root surface organize and become functional when the tooth erupts (14,15). Therefore, the space between the bone crest and cementoenamel junction may already be determined from this pre-eruptive stage.…”

Section: Discussionmentioning

confidence: 99%

Morphology and dimensions of the dentogingival unit in the altered passive eruption

Alpiste-Illueca¹

2012

Med Oral

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Objetives: This study define altered passive eruption (APE) and evaluate the morphology of the dentogingival unit. Material and Methods: 123 individuals subjected to clinical examination and parallel profile radiography of the upper central incisor. An evaluation was made of the correlation between the clinical diagnosis of APE and the degree of gingival overlap; by using a 19% overlap to define APE (Kappa concordance index = 0.7). The Mann-Whitney / Wilcoxon test was used to identify the variables influencing APE. Results: Statistically significant differences were observed between the teeth with and without APE: gingival width (p = 0.0073), clinical crown length (p = 0.0000), smiling exposed gums (p = 0.0000), bone crest thickness (p = 0.0030), connective tissue attachment thickness (p = 0.0003) and biological width (p = 0.0015). Conclusions: The APE is characterized by: a gingival overlapping of over 19% of the length of the anatomical crown, increased gingival width and gingival smile; furthermore is associated to a thick bone crest and connective tissue attachment. Statistical analysis confirms two morphological patterns of APE. Key words:Altered passive eruption (ape), radiographic exploration, dentogingival unit (dgu), gingival thickness, plastic periodontal surgery, surgical crown lengthening.

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“…The dental follicle develops from ectomesenchyme. It surrounds the developing tooth germ before eruption (Ten Cate, 1997;Wise, 2002). During embryonic development, the ectomesenchyme is partly derived from migrating cells of the cranial neural crest.…”

Section: Stem Cells For Bone Regenerationmentioning

confidence: 99%