The Development of the Thalamic Motor Learning Area Is Regulated by Fgf8 Expression

Martínez-Ferre, Almudena; Martı́nez, Salvador

doi:10.1523/jneurosci.2625-09.2009

Cited by 52 publications

(61 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Epithalamic Fgf signaling (Fgf8a and Fgf17) could play a similar morphogenic role in specifying pineal complex cell types. Fgf8 knockout mice exhibit severe deficits in all epithalamic cell types, including the pineal organ 329 RESEARCH ARTICLE Fgf and parapineal fate Martinez-Ferre and Martinez, 2009), precluding the analysis of how different cell fates are specified; in the zebrafish, the pineal complex is largely intact, allowing us to examine the role of Fgf signaling in the generation of pineal complex subtypes. Our data suggest that Fgf signaling does not act as a morphogen in the pineal complex, i.e.…”

Section: Discussionmentioning

confidence: 99%

Fgf signaling governs cell fate in the zebrafish pineal complex

2013

View full text Add to dashboard Cite

SUMMARYLeft-right (L-R) asymmetries in neuroanatomy exist throughout the animal kingdom, with implications for function and behavior. The molecular mechanisms that control formation of such asymmetries are beginning to be understood. Significant progress has been made by studying the zebrafish parapineal organ, a group of neurons on the left side of the epithalamus. Parapineal cells arise from the medially located pineal complex anlage and migrate to the left side of the brain. We have found that Fgf8a regulates a fate decision among anterior pineal complex progenitors that occurs just prior to the initiation of leftward migration. Cell fate analysis shows that in the absence of Fgf8a a subset of cells in the anterior pineal complex anlage differentiate as cone photoreceptors rather than parapineal neurons. Fgf8a acts permissively to promote parapineal fate in conjunction with the transcription factor Tbx2b, but might also block cone photoreceptor fate. We conclude that this subset of anterior pineal complex precursors, which normally become parapineal cells, are bipotential and require Fgf8a to maintain parapineal identity and/or prevent cone identity.

show abstract

Section: Discussionmentioning

confidence: 99%

Fgf signaling governs cell fate in the zebrafish pineal complex

2013

View full text Add to dashboard Cite

show abstract

“…In previous studies, migration of neural precursor cells in p1 and p2 has been investigated by analyzing expression of molecular markers characteristic of each region (Ferran et al, 2008(Ferran et al, , 2009Martinez-Ferre and Martinez, 2009). To understand better the patterns of cell migration in the developing thalamus, we conducted pulse labeling of cells with BrdU at E12.5.…”

Section: Radial Migration Of Thalamic Neuronsmentioning

confidence: 99%

Dynamic spatiotemporal gene expression in embryonic mouse thalamus

Suzuki-Hirano

Ogawa

Kataoka

et al. 2010

J of Comparative Neurology

View full text Add to dashboard Cite

The anatomy of the mammalian thalamus is characterized by nuclei, which can be readily identified in postnatal animals. However, the molecular mechanisms that guide specification and differentiation of neurons in specific thalamic nuclei are still largely unknown, and few molecular markers are available for most of these thalamic subregions at early stages of development. We therefore searched for patterned gene expression restricted to specific mouse thalamic regions by in situ hybridization during the onset of thalamic neurogenesis (embryonic [E] days E10.5-E12.5). To obtain correct regional information, we used Shh as a landmark and compared spatial relationships with the zona limitans intrathalamica (Zli), the border of the p2 and p3 compartments of the diencephalon. We identified genes that are expressed specifically in the ventricular zone of the thalamic neuroepithelium and also identified a number of genes that already exhibited regional identity at E12.5. Although many genes expressed in the mantle regions of the thalamus at E12.5 showed regionally restricted patterns, none of these clearly corresponded to individual thalamic nuclei. We next examined gene expression at E15.5, when thalamocortical axons (TCAs) project from distinct regions of the thalamus and reach their targets in the cerebral cortex. Regionally restricted patterns of gene expression were again seen for many genes, but some regionally bounded expression patterns in the early postnatal thalamus had shifted substantially by E15.5. These findings reveal that nucleogenesis in the developing thalamus is associated with selective and complex changes in gene expression and provide a list of genes that may actively regulate the development of thalamic nuclei.

show abstract

“…Later, Zli is an important source of the morphogen Shh, which is crucial for further patterning of both the developing prethalamus and thalamus (Hashimoto-Torii et al, 2003;Jeong et al, 2011;Kiecker and Lumsden, 2004;Scholpp et al, 2007;Vieira et al, 2005;Vue et al, 2009). Other local signals regulating thalamic development include fibroblast growth factors and Wnts (Braun et al, 2003;Kataoka and Shimogori, 2008;Martinez-Ferre and Martinez, 2009;Zhou et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres

et al. 2012

View full text Add to dashboard Cite

SUMMARYDiverse mechanisms regulate development of GABAergic neurons in different regions of the central nervous system. We have addressed the roles of a proneural gene, Ascl1, and a postmitotic selector gene, Gata2, in the differentiation of GABAergic neuron subpopulations in three diencephalic prosomeres: prethalamus (P3), thalamus (P2) and pretectum (P1). Although the different proliferative progenitor populations of GABAergic neurons commonly express Ascl1, they have distinct requirements for it in promotion of cell-cycle exit and GABAergic neuron identity. Subsequently, Gata2 is activated as postmitotic GABAergic precursors are born. In P1, Gata2 regulates the neurotransmitter identity by promoting GABAergic and inhibiting glutamatergic neuron differentiation. Interestingly, Gata2 defines instead the subtype of GABAergic neurons in the rostral thalamus (pTh-R), which is a subpopulation of P2. Without Gata2, the GABAergic precursors born in the pTh-R fail to activate subtype-specific markers, but start to express genes typical of GABAergic precursors in the neighbouring P3 domain. Thus, our results demonstrate diverse mechanisms regulating differentiation of GABAergic neuron subpopulations and suggest a role for Gata2 as a selector gene of both GABAergic neuron neurotransmitter and prosomere subtype identities in the developing diencephalon. Our results demonstrate for the first time that neuronal identities between distinct prosomeres can still be transformed in postmitotic neuronal precursors.

show abstract

The Development of the Thalamic Motor Learning Area Is Regulated by Fgf8 Expression

Cited by 52 publications

References 68 publications

Fgf signaling governs cell fate in the zebrafish pineal complex

Fgf signaling governs cell fate in the zebrafish pineal complex

Dynamic spatiotemporal gene expression in embryonic mouse thalamus

Transcriptional regulatory mechanisms underlying the GABAergic neuron fate in different diencephalic prosomeres

Contact Info

Product

Resources

About