The developmental neurotoxicity study of platinum compounds. Effects of cisplatin versus a novel Pt(II) complex on rat cerebellum

Developmental expression of cellular prion protein and apoptotic molecules in the rat cerebellum: Effects of platinum compounds

“…The most evident effects of cisPt were: an increased expression of PrPc in the Purkinje cell somata and main proximal dendrites at PD11, together with a general weak labelling of the granule cells progenitors of EGL, where, as previously described (Cerri et al, 2011;Pisu et al, 2005), a marked increase of apoptotic bodies was found at higher 10 mg/g cisPt dose; a similar pattern was noticed in the IGL. This trend was followed by an increased and diffuse intensity in the ML where Purkinje cells extend their dendrite tree, at PD17.…”

“…Intriguingly, in these bodies, Bax and PrPc coexist. On the contrary, after PtAcacDMS, changes in PrPc and Bcl-2 with respect to the controls were found, but apoptotic bodies with strong Bax immunoreactivity were not detectable, which could mean that apoptotic cell death of proliferating cells is preserved Cerri et al, 2011). More interesting are the data on PD17, when Bcl-2 and PrPc synergistically counter cell death in the surviving EGL proliferating cells; in parallel, Bax intensity values decreased after cisPt, while they increased after PtAcacDMS (see Section 5).…”

“…On the other hand, after treatment with PtAcacDMS, PrPc immunoreactivity increased in the Purkinje cell soma, whereas no relevant cytoarchitectural changes were detectable after this treatment; the EGL proliferating cells did not undergo apoptotic cell death over the normal trend and Purkinje cell dendrite growth was not altered significantly Cerri et al, 2011).…”

“…This suggests that its preferred cell target could be amino acid residues of enzymes and other proteins involved in apoptotic induction, thus characterizing it as a compound with non-genomic targets. Recently, the low neurotoxicity of PtAcacDMS was supported by findings on cerebellum postnatal ontogenesis Cerri et al, 2011).…”

Synthesis, Crystal Structure, and Biological Study of PtII Complexes with 4‐Acyl‐5‐pyrazolones

“…[10] In this context, new Pt II complexes containing coordinate O,OЈ-acetylacetonate chelate as a carrier ligand with an intriguing chemical reactivity [11][12][13][14] and interesting biological activities have been recently reported. [15][16][17][18][19][20] In the present work, we evaluate both the Pt II coordination ability of two 4-acyl-5-pyrazolones [HQ Ph = 4-benzoyl-3-methyl-1-phenylpyrazol-5-one, a; HQ py,CF3 = 3-methyl-1-(2-pyridyl)-trifluoroacetylpyrazol-5-one, b] and the cytotoxic activity of the corresponding platinum complexes. have shown the ability to coordinate acylpyrazolone ligands, [21] reports of Pt derivatives with 5-pyrazolones, to the best of our knowledge, have been rare.…”