The Diagnosis of Urinary Tract Infection

Schmiemann, Guido; Kniehl, Eberhardt; Gebhardt, Klaus; Matejczyk, Martha M; Hummers-Pradier, Eva

doi:10.3238/arztebl.2010.0361

Cited by 336 publications

(365 citation statements)

References 33 publications

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“…1B) can be used for real time bacterial density determination (for example, in a urine sample). Within 30 s, it would be possible to assess if it was a severe bacterial infection with more than 10 5 cells per 1 mL, and within 10 min, it would be possible to determine if there is a clinically relevant infection at all (17,18).…”

Section: Resultsmentioning

confidence: 99%

Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging

Baltekin

Boucharin

Tano

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

343

359

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The emergence and spread of antibiotic-resistant bacteria are aggravated by incorrect prescription and use of antibiotics. A core problem is that there is no sufficiently fast diagnostic test to guide correct antibiotic prescription at the point of care. Here, we investigate if it is possible to develop a point-of-care susceptibility test for urinary tract infection, a disease that 100 million women suffer from annually and that exhibits widespread antibiotic resistance. We capture bacterial cells directly from samples with low bacterial counts (10 4 cfu/mL) using a custom-designed microfluidic chip and monitor their individual growth rates using microscopy. By averaging the growth rate response to an antibiotic over many individual cells, we can push the detection time to the biological response time of the bacteria. We find that it is possible to detect changes in growth rate in response to each of nine antibiotics that are used to treat urinary tract infections in minutes. In a test of 49 clinical uropathogenic Escherichia coli (UPEC) isolates, all were correctly classified as susceptible or resistant to ciprofloxacin in less than 10 min. The total time for antibiotic susceptibility testing, from loading of sample to diagnostic readout, is less than 30 min, which allows the development of a point-of-care test that can guide correct treatment of urinary tract infection.point of care | UTI | AST | antibiotic | resistance | microfluidic

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Section: Resultsmentioning

confidence: 99%

Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging

Baltekin

Boucharin

Tano

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

343

359

View full text Add to dashboard Cite

show abstract

“…Prompt and efficacious treatment of symptomatic UTIs is warranted in pregnant women. Nevertheless, there is still some controversy regarding the screening and treatment of asymptomatic forms during gestation (Lin and Brown 2010;Lumbiganon et al, 2010;Schmiemann et al, 2010). Given the prevalence and the potential impacts of UTIs on the health of the mother and her child, the objective of this chapter is to cover the most important clinical aspects of the epidemiology, etiology, physiopathology, pregnancy outcomes, and the treatment risks and benefits associated with UTIs during pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Urinary Tract Infections During Pregnancy

Berard¹,

Santos²,

Ferreira³

et al. 2011

Urinary Tract Infections

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“…2,3 The rationale of these studies is to provide clinicians with a clinical decision rule to improve the quality of UTI care. 4,5 McIsaac et al have shown that the application of a simple diagnostic algorithm can result in the correct treatment of most females with UTI and can reduce unnecessary urine culture testing and antibiotic prescriptions.…”

Section: Introductionmentioning

confidence: 99%