The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis

El-Deeb, Wael; Elmoslemany, Ahmed

doi:10.7717/peerj.2161

Cited by 24 publications

(12 citation statements)

References 39 publications

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“…This study revealed a significant elevation in the phagocytic activity (phagocytic percent and phagocytic index) in P. multocida -infected rabbits. In agreement with El-Deeb and Elmoslemany [ 49 ], proinflammatory cytokines' serum levels (TNF- α and IL-6) were markedly elevated to enhance leukocyte migration into the infection site [ 46 – 48 ]. It has been reported that IL-6 increases in the blood after infection as an inflammatory response to regulate neutrophil and monocyte transition during the inflammation process [ 50 , 51 ].…”

Section: Discussionsupporting

confidence: 69%

“…Rabbits, infected with P. multocida , showed marked reductions in serum GSH and SOD with elevation of MDA levels, reflecting the increased lipid peroxidation and production of reactive oxygen species by bacterial infection [ 49 ]. Our results indicate that allicin treatment, alone or in combination with norfloxacin, ameliorated the oxidative stress and generation of free radicals in the infected rabbits represented by amelioration of lipid peroxidation and elevation of GSH and SOD levels through its antioxidative action [ 58 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

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Anti‐Inflammatory, Immunomodulatory, and Antioxidant Activities of Allicin, Norfloxacin, or Their Combination against Pasteurella multocida Infection in Male New Zealand Rabbits

Alam

Fawzi

Alkhalf

et al. 2018

Oxidative Medicine and Cellular Longevity

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The present study investigated the efficacy of allicin as an antibacterial, anti-inflammatory, antioxidant, and immunostimulant agent in reducing the severity of Pasteurella multocida (P. multocida) type B infection in rabbits. Fifty New Zealand rabbits, 5 weeks old, were divided equally into five groups. Except for group 1, all groups were intranasally infected with P. multocida type B (2 × 105 colony forming units/ml/rabbit). Then, group 3 rabbits were orally treated with allicin (50 mg/kg BW) for 5 days, group 4 rabbits received a single oral dose of norfloxacin 30% (100 mg/kg BW), while group 5 rabbits were treated with a combination of norfloxacin and allicin. Hematological, serum biochemical, inflammatory cytokine, immunological, and histopathological analyses were performed. Results revealed that rabbits, infected with P. multocida type B, exhibited macrocytic hypochromic anemia and leukocytosis with a significant elevation in the phagocytic percentage and index. Moreover, significant reductions in serum total protein, albumin, globulin, and immunoglobulin (IgG and IgM) levels were observed in infected rabbits. Infected rabbits showed significant increases in serum inflammatory cytokine (TNF-α and IL-6), alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and serum bilirubin (total, direct, and indirect) levels. Further, P. multocida infection induced oxidative stress as demonstrated by the significant reduction in serum levels of reduced glutathione and superoxide dismutase enzyme and marked elevation in serum malondialdehyde. Treatment with allicin, norfloxacin, or their combination significantly ameliorated the alterations in all studied parameters. In conclusion, allicin could ameliorate the inflammation and oxidative stress, induced by P. multocida type B infection in rabbits.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Anti‐Inflammatory, Immunomodulatory, and Antioxidant Activities of Allicin, Norfloxacin, or Their Combination against Pasteurella multocida Infection in Male New Zealand Rabbits

Alam

Fawzi

Alkhalf

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Moreover, APP concentrations change during bacterial and parasitic infections. Infection of sheep with P. multocida , the Gram-negative bacterium, increased the concentration of Hp (34-fold), SAA (7-fold), and Fb (2-fold) [ 87 ], while infection of sheep with Sarcoptes scabei (an arthropod, the itch mite) caused the elevation of the concentration of Hp (2-5-fold), AGP (10-fold), and Cp (2-5-fold). Serum SAA, Hp, LBP, and AGP concentrations increased (approximately 10-150-fold; 10-30-fold; 2-6-fold; 2-10-fold, respectively) in goats infected with Haemonchus contortus (an abomasal blood-sucking nematode, the barber’s pole worm, one of the most pathogenic and common parasites of ruminants) [ 88 ].…”

Section: Discussionmentioning

confidence: 99%

Acute Phase Protein Levels as An Auxiliary Tool in Diagnosing Viral Diseases in Ruminants—A Review

Reczyńska

Zalewska

Czopowicz

et al. 2018

Viruses

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We examined acute phase protein (APP) concentrations in viral infections of dairy ruminants and assessed the potential role of characteristic patterns of APP changes in auxiliary diagnosing viral diseases. All viruses reviewed are common causes of farm animal diseases. APPs are among the first agents of immunity, and their concentrations could be diagnostically relevant. In the most common ruminant viral diseases, elevated serum amyloid A (SAA) and haptoglobin (Hp) levels in blood serum have been observed. However, since these proteins are the main APPs in many viral infections, it is impossible to use their levels for diagnosing particular infections. Decreased Cp and albumin expression could help differentiate the bluetongue virus infection from other diseases. Lastly, analysis of SAA levels in blood serum and milk could be helpful in diagnosing small ruminant lentivirus infection. While promising, APP levels can only be considered as an auxiliary tool in diagnosing viral diseases in ruminants.

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“…Changes in APP serum concentration in sheep have been also studied during infections or other pathological conditions [69,74,75]. For example, El-Deeb et al [69] examined APP concentration in sheep with Coxiella burnetii infection-induced abortion or sheep with pneumonic pasteurellosis [76] and concluded that determination of APP concentration can be an auxiliary tool in diagnostics of these diseases. On the other hand, Meling et al [75] described an acute phase response in sheep with clinical scrapie, while Sanchez-Cordon et al [74] determined acute phase response in the case of both experimentally and naturally occurring bluetongue and found a correlation between APP and the evolution of clinical signs and gross lesions.…”

Section: Acute-phase Proteinsmentioning

confidence: 99%

Potential Novel Biomarkers for Mastitis Diagnosis in Sheep

Libera

Konieczny

Grabska

et al. 2021

Animals

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This review aims to characterize promising novel markers of ovine mastitis. Mastitis is considered as one of the primary factors for premature culling in dairy sheep and has noticeable financial, productional, and animal welfare-related implications. Furthermore, clinical, and subclinical mammary infections negatively affect milk yield and alter the milk composition, thereby leading to lowered quality of dairy products. It is, therefore, crucial to control and prevent mastitis through proper diagnosis, treatment or culling, and appropriate udder health management particularly at the end of the lactation period. The clinical form of mastitis is characterized by abnormalities in milk and mammary gland tissue alteration or systemic symptoms consequently causing minor diagnostic difficulties. However, to identify ewes with subclinical mastitis, laboratory diagnostics is crucial. Mastitis control is primarily dependent on determining somatic cell count (SCC) and the California Mastitis Test (CMT), which aim to detect the quantity of cells in the milk sample. The other useful diagnostic tool is microbial culture, which complements SCC and CMT. However, all mentioned diagnostic methods have their limitations and therefore novel biomarkers of ovine subclinical mastitis are highly desired. These sensitive indicators include acute-phase proteins, miRNA, and cathelicidins measurements, which could be determined in ovine serum and/or milk and in the future may become useful in early mastitis diagnostics as well as a preventive tool. This may contribute to increased detection of ovine mammary gland inflammation in sheep, especially in subclinical form, and consequently improves milk quality and quantity.

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The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis

Cited by 24 publications

References 39 publications

Anti‐Inflammatory, Immunomodulatory, and Antioxidant Activities of Allicin, Norfloxacin, or Their Combination against Pasteurella multocida Infection in Male New Zealand Rabbits

Anti‐Inflammatory, Immunomodulatory, and Antioxidant Activities of Allicin, Norfloxacin, or Their Combination against Pasteurella multocida Infection in Male New Zealand Rabbits

Acute Phase Protein Levels as An Auxiliary Tool in Diagnosing Viral Diseases in Ruminants—A Review

Potential Novel Biomarkers for Mastitis Diagnosis in Sheep

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