The diagnostic-escape Finnish new variant of Chlamydia trachomatis first described in 2019 remains present in Finland three years after the introduction of an updated Aptima Combo 2 assay

Puolakkainen, Mirja; Mannonen, Laura; Unemo, Magnus

doi:10.1016/j.cmi.2023.02.009

Cited by 2 publications

(1 citation statement)

References 5 publications

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“… 97 In February 2019, a diagnostic-escape CT mutant (23S rRNA, C1515T) emerged in Finland (10% in 2019, 2% in 2022) and spread to Norway (84% prevalence in 2020), while another escape mutant (23S rRNA, G1523A) became prevalent in Denmark (95% in 2020). 98 Moreover, specific LGV CT strains, have been identified as the cause of recent epidemics among men who have sex with men, as demonstrated through genotyping of the outer-membrane-protein (OmpA). 99 An LGV epidemic was caused by the L2b variant in 2004 in Europe, and new variants have been identified later, but all derive from the initial L2b strain.…”

Section: Molecular Epidemiology: Network Of Transmission and Clusteringmentioning

confidence: 99%

Epidemiology and determinants of reemerging bacterial sexually transmitted infections (STIs) and emerging STIs in Europe

Mitjà,

Padovese,

Folch

et al. 2023

The Lancet Regional Health - Europe

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Section: Molecular Epidemiology: Network Of Transmission and Clusteringmentioning

confidence: 99%

Epidemiology and determinants of reemerging bacterial sexually transmitted infections (STIs) and emerging STIs in Europe

Mitjà,

Padovese,

Folch

et al. 2023

The Lancet Regional Health - Europe

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Diagnostic-avoidingChlamydia trachomatisvariants detected in cervical and endometrial specimens from women during 16S microbiome profiling

Jeong,

Tollison,

Brochu

et al. 2024

Preprint

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Background: Performance of a 16S rRNA analysis of the cervicovaginal microbiome of 220 participants recruited into the T Cell Response against Chlamydia (TRAC) cohort between February 2011 and August 2014 in Allegheny County, Pennsylvania USA surprisingly detected DNA encoding chlamydial 16S rRNA in samples from seven participants who had tested negatively for Chlamydia trachomatis (CT) and DNA encoding gonococcal 16S rRNA from five participants who had tested negatively for Neisseria gonorrhoeae (NG) infection with the Aptima Combo2 assay (Hologic). Methods: We used targeted PCR amplification followed by sequencing to characterize the chlamydial 23S rRNA locus and qPCR to detect gonococcal DNA in residual diagnostic swab eluates or DNA used to generate 16S rRNA libraries. Results: Discrepant specimens that contained chlamydial DNA carried a diagnostic-avoidant, G1526A variant in the 23S rRNA locus identical to variants previously detected in Finland, Denmark, and the UK. PCR validation of gonococcal DNA was confirmed for all participants who had tested negatively, with stochastic effects consistent with infection levels close to the limit of detection by the diagnostic assay. Conclusions: These data indicate that this probe-avoidant CT mutant, and possibly others, were circulating in the northeastern US prior to their detection and characterization in 2019 and subsequently. Although infrequent, documentation of false negative results for CT indicates a need for clinicians to consider performance of a second test that uses alternate PCR probes if patients have persistent symptoms or have known contact to an infected sex partner and their initial NAAT is negative.

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The diagnostic-escape Finnish new variant of Chlamydia trachomatis first described in 2019 remains present in Finland three years after the introduction of an updated Aptima Combo 2 assay

Cited by 2 publications

References 5 publications

Epidemiology and determinants of reemerging bacterial sexually transmitted infections (STIs) and emerging STIs in Europe

Epidemiology and determinants of reemerging bacterial sexually transmitted infections (STIs) and emerging STIs in Europe

Diagnostic-avoidingChlamydia trachomatisvariants detected in cervical and endometrial specimens from women during 16S microbiome profiling

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