The diagnostic reliability of urinary cytology: A retrospective study

Abstract

The aim of this study was twofold. The first aim was to estimate the diagnostic reliability of urinary cytology for detection and management of urothelial neoplasms by using a specific preserving fluid for sample collection, and the liquid-based thin layer method for specimen preparation, the estimate was based on the correlation between the cytological findings of 10,000 non-hospitalized patients, and their histological diagnoses. A second aim was to compare the reliability of two instruments for thin-layer preparation, i.e., TP2000, TP3000, capable of processing the specimens at very different rates. The preservation of cell structure is ameliorated by the procedure of sample collection and treatment here described. This allows a more accurate reading of LBC slides as shown by: (a) the significant concordance between cytological and histological diagnosis (92%); (b) the significant number of low-grade urothelial carcinomas (20.5%) revealed by urinary cytology and validated by histologic diagnosis; (c) the low rate (8%) of misjudgement of cytological diagnosis reached in this study. The quality of performances of the two instruments tested for thin-layer preparation, i.e., TP2000 and TP3000, is statistically comparable. We recommend the procedure that makes use of preserving fluid for sample collection (cytolyt™) and treatment (preservcyt ™) as here described. We also recommend the use of thin-layer method for specimen preparation since it allows a more uniform distribution of the cells on the support with reduction of overlapping phenomena. Finally, economic considerations suggest the preferential use of Thin Prep 3000.

Diagnostic Value of Liquid-Based Cytology in Urothelial Carcinoma Diagnosis: A Systematic Review and Meta-Analysis

“…Recently, this slide-making technique has been noted in other medical fields, such as breast cancer, sputum cytology of lung cancer, and especially urinary cytology. Some studies indicate that LBC may improve the sensitivity of bladder cancer detection and the background of micrographs [ 4 – 7 ]. We evaluated the diagnostic value of the LBC techniques in urothelial carcinoma with evidence-based medicine methods.…”

Comparison of urine cytology diagnostic reports before and after the implementation of the Paris System classification system in China

“…Urine cytology has long been criticised for the lack of standardised diagnostic criteria and unified reporting terminology . In 2013, TPS was created to improve the diagnostic accuracy of urine cytology and standardise the reporting terminology …”

Oral hairy leukoplakia diagnosis by Epstein–Barr virus in situ hybridization in liquid‐based cytology

“…Fewer studies have used LBC on oral mucosal lesions in comparison with those of the uterine cervix. In situ techniques, such as hybridization, may be applied to LBC more easily, as the slide preparations are more homogeneous and cells are concentrated in a small area, a 25‐mm circle in ThinPrep, saving reagents …”

“…The material was collected from the lateral border of the tongue of the three groups by the same researcher using the ThinPrep Pap Test, which contains a conical cytobrush, transportation and cell conservation‐medium tube (PreserveCyt ® ), which is an isotonic saline solution containing 20% methyl alcohol . The cytobrush was scraped 10 times over the area of choice, rinsed in the medium and conserved at 4 °C for subsequent slide preparation.…”

“…A gentle vacuum collects cells on the exterior surface of the filter membrane. After the cells have been collected, the filter is inverted and gently pressed against the microscope slide . The thin layer was fixed with absolute ethyl alcohol and, for each case, one slide was stained according to the Papanicolaou (Pap) method and another was submitted to EBV in situ hybridization.…”