The diagnostic significance of blood-derived circRNAs in NSCLC: Systematic review and meta-analysis

Abstract

BackgroundUsing blood-derived circular RNAs (circRNAs) may be an efficient tool for noninvasive fluid biopsy in diagnosing non-small cell lung cancer (NSCLC). However, no relevant systemic meta-analysis has been conducted so far to support the diagnostic value of using blood-derived circRNAs in NSCLC clinically. The aim of this study is to clarify the issue through a meta-analysis.MethodsA systematic search strategy was used to search relevant literature in the databases of PubMed, Web of Science, and Cochrane Library from 2017 to 2022. The relationship between the diagnostic accuracy of circRNAs and NSCLC was analyzed. For the purpose of evaluating the quality of the literature, Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) was used. Statistical analyses were assessed using Stata software (version 17.0) and META-DISC (version 1.4).ResultsThe meta-analysis included 1,093 patients with NSCLC and 959 controls. Results are as follows: pooled sensitivity, 0.78 (95% CI = 0.71–0.83, I2 = 71.86); pooled specificity, 0.76 (95% CI = 0.70–0.82, I2 = 70.12); pooled positive likelihood ratio (PLR), 3.3 (95% CI = 2.6–4.2, I2 = 37.56); pooled negative likelihood ratio (NLR), 0.29 (95% CI = 0.23–0.37, I2 = 64.67); diagnostic odds ratio (DOR), 11.42 (95% CI = 7.88–16.56, I2 = 99.05); area under the receiver operating characteristic curve (AUC), 0.84 (95% CI = 0.80–0.87). Based on the subgroup analysis, it appears that the heterogeneity is primarily caused by the NSCLC subgroup.ConclusioncircRNAs are highly useful diagnostic biomarkers for NSCLC in China. Further prospective studies on the diagnostic value of circRNAs should be conducted in multiple countries.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022323804.

Circ_0000877 accelerates proliferation and immune escape of non‐small cell lung cancer cells by regulating microRNA‐637/E2F2 axis

“…CircRNAs are a new class of non‐linear, single‐stranded and non‐coding RNAs with favorable biological stability and tissue cell specificity, which may be served as possible biomarkers for cancer diagnosis and prognosis 28 . Currently, the bulk of the researches suggested that circRNAs played vital role in regulating the progression of NSCLC 29,30 . Zhang et al discovered the up‐expressed circSATB2 in serumal exosomes from lung cancer patients, which was linked to the promotion of NSCLC proliferation, migration, and invasion 31 .…”

“…28 Currently, the bulk of the researches suggested that cir-cRNAs played vital role in regulating the progression of NSCLC. 29,30 Zhang et al discovered the up-expressed circSATB2 in serumal exosomes from lung cancer patients, which was linked to the promotion of NSCLC proliferation, migration, and invasion. 31 Herein, we also noticed that circ_0000877 was up-regulated in NSCLC tissues and cells, circ_0000877 knockdown obviously prohibited NSCLC cell proliferation and accelerated apoptosis.…”