ABSTRACTactive malignancy or previous chemotherapy for a disease other than CLL. All donors gave written informed consent. Ethical approval for the study was obtained from the Central Regional Ethics Committee of New Zealand.Binet and Rai prognostic scores were determined from the clinical examination and absolute differential blood counts (Sysmex XE 2100 analyzer, Roche Diagnostics, Auckland, New Zealand) measured at the time of blood sampling. Details of peripheral blood mononuclear cell (PBMC) isolation and storage, and B-cell depletion and enrichment are given in the Online Supplementary Design and Methods.