The diagnostic value of the measurement of matrix metalloproteinase 9 (MMP-9), squamous cell cancer antigen (SCC) and carcinoembryonic antigen (CEA) in the sera of esophageal cancer patients

Mroczko, Barbara; Kozłowski, Mirosław; Groblewska, Magdalena; Łukaszewicz, Marta; Nikliński, Jacek; Jelski, Wojciech; Laudański, J; Chyczewski, L; Szmitkowski, Maciej

doi:10.1016/j.cca.2007.11.023

Cited by 80 publications

(66 citation statements)

References 13 publications

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“…The recent study of our research team has shown that the preoperative serum levels of MMP-9 in esophageal cancer patients were statistically higher than in healthy subjects, and correlated with clinical stage of disease as well as with tumor size [46] (Figure 1). The diagnostic sensitivity of serum MMP-9 measurement was higher (70%) than for classical tumor markers (CEA -17% and SCC-Ag -64%) and increased in the combination of MMP-9 with SCC-Ag [46].…”

Section: Mmp-9mentioning

confidence: 91%

“…It has been proved that these enzymes are involved in the development of many malignant neoplasms: gastric, colorectal, pancreatic and breast cancer [40][41][42][43]. High MMP-9 protein expression in tumor tissues of EC patients has been shown in several studies [4,18,[44][45][46][47]. Percentages of MMP-9 positive immunostaining (diagnostic sensitivity) in esophageal cancer tissue reached 78% in the study by Murray et al [44] and increased with tumor stage.…”

Section: Mmp-9mentioning

confidence: 99%

“…The diagnostic sensitivity of serum MMP-9 measurement was higher (70%) than for classical tumor markers (CEA -17% and SCC-Ag -64%) and increased in the combination of MMP-9 with SCC-Ag [46].…”

Section: Mmp-9mentioning

confidence: 99%

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The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer

Groblewska

Siewko

Mroczko

et al. 2012

Folia Histochem Cytobiol.

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173

108

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Esophageal cancer (EC) is one of the most aggressive malignant tumors of the gastrointestinal tract. There are two distinct histological types of EC: esophageal squamous cell carcinoma and adenocarcinoma of the esophagus. Etiologic factors and the patterns of incidence of both subtypes are different. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play an important role in esophageal carcinogenesis. Gellatinases MMP-2 and MMP-9 are able to degrade collagen IV from basement membranes and extracellular matrix which is related to tumor progression, including invasion, metastasis, growth and angiogenesis. It has been shown that increased expression of MMPs plays a crucial role in the development of several human malignancies, including esophageal cancer. The activity of MMPs is regulated by their endogenous natural inhibitors (TIMPs). Among these, the roles of TIMP-1 and TIMP-2 in EC development, tumor progression and formation of metastases have been most extensively characterized and best recognized.

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Section: Mmp-9mentioning

confidence: 91%

Section: Mmp-9mentioning

confidence: 99%

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The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer

Groblewska

Siewko

Mroczko

et al. 2012

Folia Histochem Cytobiol.

Self Cite

173

108

View full text Add to dashboard Cite

show abstract

“…The positive results of MMP-2 and TIMP-2 are below cut-off values. The reference cut-off value for CEA and SCC were established previously in our department [21]. The cut-off points were 4.0 ng/mL for CEA and 2.0 ng/mL for SCC.…”

Section: Methodsmentioning

confidence: 99%

Serum matrix metalloproteinase 2 and tissue inhibitor of matrix metalloproteinases 2 in esophageal cancer patients

Groblewska¹,

Mroczko²,

Kozłowski³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:The positive expression of MMP-2 and TIMP-2 were found in esophageal cancer (EC) tissue and correlated with cancer stage and clinico-pathological features of tumor and patients' survival. However, little is known about serum levels of those proteins in EC patients. The aim of the present study was to investigate the diagnostic significance of MMP-2 and TIMP-2 serum levels in EC patients in relation to clinico-pathological features of cancer. The study included 53 EC patients and 92 healthy controls. The serum levels of MMP-2, TIMP-2 and classical tumor markers CEA (carcinoembryonic antigen) and SCC (squamous cell carcinoma antigen) were assayed. The prognostic values and diagnostic criteria for the biomarkers tested were defined. Serum levels of MMP-2, TIMP-2 in EC patients were significantly lower, whereas CEA and SCC significantly higher than in control group. The diagnostic sensitivity of TIMP-2 (57%) was higher than those for other biomarkers tested and increased in combination with SCC (70%). Area under ROC curve for TIMP-2 (0.8698) was larger than for other proteins. In Cox's univariate analysis only SCC serum levels were significant prognostic factors for EC patients' survival. The results suggest the limited value of serum analyses of MMP-2 for tumor staging and prognosis in EC and the better usefulness of TIMP-2 than MMP-2 as a tumor marker in the diagnosis of EC, especially in combined use with SCC.

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“…Of MMPs, MMP9 is implicated in various pathological conditions including diabetes (Giebel et al 2005), hypertension (Berk et al 2007), and cancers (Martin and Matrisian 2007). High level of MMP9 has been detected in various body fluids taken from humans with various tumors (Fernández et al 2005;Hurst et al 2007;Eissa et al 2007;Mroczko et al 2008;Shpitzer et al 2009). MMP9 not only promotes tumor angiogenesis (Yu and Stamenkovic 2000;Riedel et al 2000) but also regulates cell adhesion by the hydrolysis of ECM resulting in tumor invasion and metastasis (Sternlicht and Werb 2001).…”

Section: Introductionmentioning

confidence: 99%

Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

Lin

et al. 2017

Appl Nanosci

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Exact assay of matrix metalloproteinase 9 (MMP9) has attracted considerable attentions for the clinical diagnosis of disease in early stage. In this report, we covalently engineer a fluorescein isothiocyanate-labeled peptide (Pep-FITC) linker containing the specific cleavage substrate of MMP9 onto the surface of hydrothermally reduced nano-graphene oxide (nrGO) to develop a FRETbased nrGO-Pep-FITC nanoprobe for ultrasensitive detection of MMP9. Upon cleavage of the Pep-FITC at the amide bond between Ser and Leu by MMP9, FITC was separated from nrGO, and fluorescence recovery of FITC was proportional to the MMP9 concentration within 0.01-0.06 nM and 0.06-0.15 nM ranges, respectively, in an aqueous solution, exhibiting 0.83 pM of detection limit. This nanoprobe is stable under borate buffer (pH = 7.4) with bovine serum albumin or 0.1% surfactant Triton 100 or Tween 20, and has good specificity for MMP9 detection, which is meaningful for MMP9-related clinical and bioanalytical applications.

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The diagnostic value of the measurement of matrix metalloproteinase 9 (MMP-9), squamous cell cancer antigen (SCC) and carcinoembryonic antigen (CEA) in the sera of esophageal cancer patients

Cited by 80 publications

References 13 publications

The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer

The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer

Serum matrix metalloproteinase 2 and tissue inhibitor of matrix metalloproteinases 2 in esophageal cancer patients

Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

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