The Different Faces of Arabidopsis arenosa—A Plant Species for a Special Purpose

Gieroń, Żaneta; Sitko, Krzysztof; Małkowski, Eugeniusz

doi:10.3390/plants10071342

Cited by 3 publications

(2 citation statements)

References 112 publications

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“…The hub gene AT1G71210, identified in the lightblue4 module of A. thaliana, codified for a pentatricopeptide repeat (PPR) superfamily protein. This gene in soybean is regulated under heat stress conditions [66], while in Arabidopsis arenosa, a species closely related to A. halleri and able to hyperaccumulate zinc and Cd, is part of a group of five genes that are under a convergent selective sweep between the metallicolous and non-metallicolous populations [67]. However, despite this, no evidence supports any description of its role in A. thaliana, including the information retrieved by ThaleMine even if the pentatricopeptide repeat proteins are described as strongly involved in abiotic stress responses [68].…”

Section: Discussionmentioning

confidence: 99%

Key Pathways and Genes of Arabidopsis thaliana and Arabidopsis halleri Roots under Cadmium Stress Responses: Differences and Similarities

Sferra

Fantozzi

Scippa

et al. 2023

Plants

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Cadmium (Cd) is among the world’s major health concerns, as it renders soils unsuitable and unsafe for food and feed production. Phytoremediation has the potential to remediate Cd-polluted soils, but efforts are still needed to develop a deep understanding of the processes underlying it. In this study, we performed a comprehensive analysis of the root response to Cd stress in A. thaliana, which can phytostabilize Cd, and in A. halleri, which is a Cd hyperaccumulator. Suitable RNA-seq data were analyzed by WGCNA to identify modules of co-expressed genes specifically associated with Cd presence. The results evidenced that the genes of the hyperaccumulator A. halleri mostly associated with the Cd presence are finely regulated (up- and downregulated) and related to a general response to chemical and other stimuli. Additionally, in the case of A. thaliana, which can phytostabilize metals, the genes upregulated during Cd stress are related to a general response to chemical and other stimuli, while downregulated genes are associated with functions which, affecting root growth and development, determine a deep modification of the organ both at the cellular and physiological levels. Furthermore, key genes of the Cd-associated modules were identified and confirmed by differentially expressed gene (DEG) detection and external knowledge. Together, key functions and genes shed light on differences and similarities among the strategies that the plants use to cope with Cd and may be considered as possible targets for future research.

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Section: Discussionmentioning

confidence: 99%

Key Pathways and Genes of Arabidopsis thaliana and Arabidopsis halleri Roots under Cadmium Stress Responses: Differences and Similarities

Sferra

Fantozzi

Scippa

et al. 2023

Plants

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“…Arabidopsis arenosa , which has both diploid and autotetraploid natural populations, is an outcrossing relative of the diploid, selfing A. thaliana ( Al-Shehbaz and O’Kane 2002 ), which makes A. arenosa an ideal subject for evolutionary studies ( Yant and Bomblies 2017 ; Koch 2019 ; Gieroń et al . 2021 ).…”

Section: Determining the Prevalence And Underlying Genetics Of Phenot...mentioning

confidence: 99%

Deleterious phenotypes in wild Arabidopsis arenosa populations are common and linked to runs of homozygosity

Barragan,

Collenberg,

Schwab

et al. 2023

G3: Genes, Genomes, Genetics

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In this study, we aimed to systematically assess the frequency at which potentially deleterious phenotypes appear in natural populations of the outcrossing model plant Arabidopsis arenosa, and to establish their underlying genetics. For this purpose, we collected seeds from wild A. arenosa populations and screened over 2,500 plants for unusual phenotypes in the greenhouse. We repeatedly found plants with obvious phenotypic defects, such as small stature and necrotic or chlorotic leaves, among first-generation progeny of wild A. arenosa plants. Such abnormal plants were present in about 10% of maternal sibships, with multiple plants with similar phenotypes in each of these sibships, pointing to a genetic basis of the observed defects. A combination of transcriptome profiling, linkage mapping and genome-wide runs of homozygosity patterns using a newly assembled reference genome indicated a range of underlying genetic architectures associated with phenotypic abnormalities. This included evidence for homozygosity of certain genomic regions, consistent with alleles that are identical by descent being responsible for these defects. Our observations suggest that deleterious alleles with different genetic architectures are segregating at appreciable frequencies in wild A. arenosa populations.

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Stable Artificial Autopolyploids of the Zn/Cd Accumulator Arabidopsis arenosa—A Promising Genetic Resource for Phytoremediation

et al. 2023

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Arabidopsis arenosa is a good candidate for phytoremediation due to its high tolerance to Zn and Cd as well as its accumulation ability. However, its small size and low biomass are the largest obstacles to applying it on a broad scale. The aim was to obtain polyploid specimens, which tend to have higher biomass to increase the accumulation and translocation capacity of heavy metals in this metal-tolerant plant. Doubled polyploids (octaploids) were obtained via indirect organogenesis on a ½ MS medium supplemented with 1 mg L−1 TDZ, followed by rooting on the same medium without growth regulators. Callus tissue of a high endopolyploidy level (the (Ʃ>2C)/2C ratio over 2.5) obtained on seedling fragments on ½ MS supplemented with 2 mg L−1 2,4-D + 2 mg L−1 BAP served as a source material. Among the regenerants successfully obtained (without using antimitotic agents), over half, regardless of the stage of regenerant development, were octaploid (54–78%; 2C DNA = 1.642 pg). Octaploids were not affected by ploidy or in vitro culture conditions; they were fully fertile, produced normal pollen (~97% of viability), and set seeds capable of germinating (78%). Their cell and organ size was affected by genome doubling resulting in longer stomata, bigger pollen grains, and flowers with a larger area and width in comparison with tetraploid regenerants and initial plants. The promising results of measurements of morpho-anatomical, physiological, and reproductive parameters indicate that, in the future, after passing tolerance tests, the obtained polyploids could be used in phytoremediation of metal-contaminated areas.

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The Different Faces of Arabidopsis arenosa—A Plant Species for a Special Purpose

Cited by 3 publications

References 112 publications

Key Pathways and Genes of Arabidopsis thaliana and Arabidopsis halleri Roots under Cadmium Stress Responses: Differences and Similarities

Key Pathways and Genes of Arabidopsis thaliana and Arabidopsis halleri Roots under Cadmium Stress Responses: Differences and Similarities

Deleterious phenotypes in wild Arabidopsis arenosa populations are common and linked to runs of homozygosity

Stable Artificial Autopolyploids of the Zn/Cd Accumulator Arabidopsis arenosa—A Promising Genetic Resource for Phytoremediation

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