The Differential Antiviral Activities of Chicken Interferon α (ChIFN-α) and ChIFN-β are Related to Distinct Interferon-Stimulated Gene Expression

Qu, Hongren; Yang, Limin; Meng, Shanshan; Xu, Lei; Bi, Yuhai; Jia, Xiaojuan; Li, Jing; Sun, Lei; Chen, Li

doi:10.1371/journal.pone.0059307

Cited by 48 publications

(47 citation statements)

References 63 publications

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“…Cell samples were collected at 0, 4, 8, and 12 h p.i. (hours post-infection) for RNA isolation as previously described [31]. The RNA samples were treated with DNase I (TaKaRa) at 37°C for 30 min.…”

Section: Rna Extraction and Quantification By Real-time Pcrmentioning

confidence: 99%

Influenza A Virus-induced expression of ISG20 inhibits viral replication by interacting with nucleoprotein

Yang

et al. 2016

Virus Genes

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Influenza A virus (IAV) is an important pathogen that has a wide range of hosts and represents a threat to the health of humans and several animal species. IAV infection can induce the transcription of many genes in the host. In the present study, we demonstrated for the first time that three different strains of H1N1 IAV induce the expression of an IFN-stimulated gene, ISG20. We determined the antiviral activity of ISG20 against IAV because ISG20 inhibited viral protein expression and reduced the progeny viral titer dependent upon its exonuclease activity. To elucidate the detailed mechanism of ISG20, we further demonstrated that ISG20 impairs the polymerase activity and inhibits both the replication and transcription levels of the M1 and NP genes. Notably, we identified that ISG20 colocalizes and interacts with NP during IAV infection, while exonuclease-inactive mutant ISG20 lacked association with NP, indicating that ISG20 inhibits IAV replication by interacting with NP. Together, these data provide a detailed explanation for the specific antiviral action of ISG20 and suggest that ISG20 may act as a promising antiviral drug candidate against IAV.

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Section: Rna Extraction and Quantification By Real-time Pcrmentioning

confidence: 99%

Influenza A Virus-induced expression of ISG20 inhibits viral replication by interacting with nucleoprotein

Yang

et al. 2016

Virus Genes

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“…The antiviral activity and anti-proliferation activity of IFNβ is lower than most of the IFNαs, and the antiviral activity of IFNα-evoluted IFNs is stronger than that of IFNκ and IFNεs (Sang et al, 2010;Lavoie et al, 2011). The ISGs that IFNβ induces tend to regulate host immunoreactions, while IFNα tends to induce antiviral proteins, such as 2′-5′ OAS and PKR (Qu et al, 2013). This may be caused by the different ways that they interact with IFN receptor subunits.…”

Section: Research Articlementioning

confidence: 99%

Distinct evolution process among type I interferon in mammals

Yang

Liu

2013

Protein Cell

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Interferon (IFN) is thought to play an important role in the vertebrate immune system, but systemic knowledge of IFN evolution has yet to be elucidated. To evaluate the phylogenic distribution and evolutionary history of type I IFNs, 13gen omes were searched using BLASTn program, and a phylogenetic tree of vertebrate type I IFNs was constructed. In the present study, an IFNδ-like gene in the human genome was identifi ed, refuting the concept that humans have no IFNδ genes, and other mammalian IFN genes were also identifi ed. In the phylogenetic tree, the mammalian IFNβ, IFNε, and IFNκ formed a clad e sepa rate f rom the other mammalian type I IFNs, while piscine and avian IFNs formed distinct clades. Based on this phylogenetic analysis and the various characteristics of type I IFNs, the evolutionary history of type I IFNs was further evaluated. Our data indicate that an ancestral IFNα-like gene forms a core from which new IFNs divided during vertebrate evolution. In addition, the data suggest how the other type I IFNs evolved from IFNα and shaped the complex type I IFN system. The promoters of type I IFNs were conserved among different mammals, as well as their genic regions. However, the intergenic regions of type I IFN clusters were not conserved among different mammals, demonstrating a high selec tion pressure upon type I IFNs during their evolution.

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“…In addition, the pretreatment of cells with interferon-β can sometimes enhance the IFN yield (an effect called priming) (Honda et al, 2006). The Qu et al (2013) found that the adding of ChIFN-β (chicken IFN-β) increase the IFN at the beginning of treatment, reaching peak level at 12 hours and then decreased. The positive control clearly had the highest gene expression at 12, 24, 36 hpi of M gene but decline at 48 hpi due to the damage of infected cells as mention by Kumar et al (2013).…”

Section: Qrt -Pcrmentioning

confidence: 99%

Antiviral Effects of Olea europaea Leaves Extract and Interferon-beta on Gene Expression of Newcastle Disease Virus

Salih¹,

Odisho²,

Al-Shammari³

et al. 2017

Adv. Anim. Vet. Sci.

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| In this study, we investigated the antiviral activity of Olea europaea (OLE) extracts against Newcastle disease virus (NDV), a highly infectious and economically important poultry virus. For this purpose, viral gene expressions of two structural proteins, matrix (M) and fusion (F) of NDV, were monitoring in chicken fibroblast cells and the impact on virus replication was analyzed using qRT-PCR. Additionally, the virus-restriction activities of IFN-β were analyzed by assessing the expression of viral and host genes. In vitro analysis of the NDV replication revealed that treatment of chicken cells with OLE significantly restricted the NDV replication as was measured by haemagglutination (HA) and titration (TCID50) assays in a pre-optimized OLE-mediated cytotoxicity levels (1000ug/ml). Correspondingly, pretreatment of chicken cells with exogenous IFN-β markedly down-regulated viral gene expression, and this regulation was higher than OLE-mediated virus replication. Furthermore, the impact of OLE on NDV was independent of the IFN-β and was confirmed using ELISA. Taken together, findings of this study propose the utilization of OLE as immunoprophylaxis and exploitation of this function to control the ND in poultry and pet birds.

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The Differential Antiviral Activities of Chicken Interferon α (ChIFN-α) and ChIFN-β are Related to Distinct Interferon-Stimulated Gene Expression

Cited by 48 publications

References 63 publications

Influenza A Virus-induced expression of ISG20 inhibits viral replication by interacting with nucleoprotein

Influenza A Virus-induced expression of ISG20 inhibits viral replication by interacting with nucleoprotein

Distinct evolution process among type I interferon in mammals

Antiviral Effects of Olea europaea Leaves Extract and Interferon-beta on Gene Expression of Newcastle Disease Virus

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