The differential assimilation of nitrogen fertilizer compounds by soil microorganisms

Abstract

The differential soil microbial assimilation of common nitrogen (N) fertilizer compounds into the soil organic N pool is revealed using novel compound-specific amino acid (AA) 15N-stable isotope probing. The incorporation of fertilizer 15 N into individual AAs reflected the known biochemistry of N assimilation—e.g. 15N-labelled ammonium (15NH4+) was assimilated most quickly and to the greatest extent into glutamate. A maximum of 12.9% of applied 15NH4+, or 11.7% of ‘retained’ 15NH4+ (remaining in the soil) was assimilated into the total hydrolysable AA pool in the Rowden Moor soil. Incorporation was lowest in the Rowden Moor 15N-labelled nitrate (15NO3−) treatment, at 1.7% of applied 15 N or 1.6% of retained 15 N. Incorporation in the 15NH4+ and 15NO3− treatments in the Winterbourne Abbas soil, and the 15N-urea treatment in both soils was between 4.4 and 6.5% of applied 15 N or 5.2 and 6.4% of retained 15 N. This represents a key step in greater comprehension of the microbially-mediated transformations of fertilizer N to organic N and contributes to a more complete picture of soil N-cycling. The approach also mechanistically links theoretical/pure culture derived biochemical expectations and bulk level fertilizer immobilization studies, bridging these different scales of understanding.