The differential effects of high-fat and high fructose diets on the liver of male albino rat and the proposed underlying mechanisms

Zaki, Sherif Mohamed; Fattah, Shereen Abdel; Hassan, Dina Salah

doi:10.5603/fm.a2018.0063

Cited by 17 publications

(24 citation statements)

References 38 publications

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“…IGF1 appears to be one of the most significant miR-379 target genes with regard to promoting the development and progression of NAFLD via the enhancement of cholesterol lipotoxicity. Among other keyword-selected putative target genes, B-cell lymphoma 2 (BCL2), catalase (CAT), and cAMP responsive element binding protein 1 (CREB1) are reportedly down-regulated in the liver in NAFLD [36,103,104]. BCL2 and CAT are major anti-apoptosis genes that function by protecting against mitochondrial outer membrane permeabilization and detoxifying ROS, respectively [36,103].…”

Section: Discussionmentioning

confidence: 99%

“…Among other keyword-selected putative target genes, B-cell lymphoma 2 (BCL2), catalase (CAT), and cAMP responsive element binding protein 1 (CREB1) are reportedly down-regulated in the liver in NAFLD [36,103,104]. BCL2 and CAT are major anti-apoptosis genes that function by protecting against mitochondrial outer membrane permeabilization and detoxifying ROS, respectively [36,103]. Down-regulation of BCL2 and CAT expression in liver tissue drives hepatocyte apoptosis, which is an important pathologic event in the development and progression of NAFLD.…”

Section: Discussionmentioning

confidence: 99%

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Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

et al. 2020

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Introduction Non-alcoholic fatty liver disease (NAFLD) has a wide spectrum, eventually leading to cirrhosis and hepatic carcinogenesis. We previously reported that a series of microRNAs (miR-NAs) mapped in the 14q32.2 maternally imprinted gene region (Dlk1-Dio3 mat) are related to NAFLD development and progression in a mouse model. We examined the suitability of miR-379, a circulating Dlk1-Dio3 mat miRNA, as a human NAFLD biomarker. Methods Eighty NAFLD patients were recruited for this study. miR-379 was selected from the putative Dlk1-Dio3 mat miRNA cluster because it exhibited the greatest expression difference between NAFLD and non-alcoholic steatohepatitis in our preliminary study. Real-time PCR was used to examine the expression levels of miR-379 and miR-16 as an internal control. One patient was excluded due to low RT-PCR signal. Results Compared to normal controls, serum miR-379 expression was significantly up-regulated in NAFLD patients. Receiver operating characteristic curve analysis suggested that miR-379 is a suitable marker for discriminating NAFLD patients from controls, with an area under the PLOS ONE |

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

et al. 2020

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“…IGF1 appears to be one of the most significant miR-379 target genes with regard to promoting the development and progression of NAFLD via the enhancement of cholesterol lipotoxicity. Among other keyword-selected putative target genes, B-cell lymphoma 2 ( BCL2 ), catalase ( CAT ), and cAMP responsive element binding protein 1 ( CREB1 ) are reportedly down-regulated in the liver in NAFLD [30, 97, 98]. BCL2 and CAT are major anti-apoptosis genes that function by protecting against mitochondrial outer membrane permeabilization and detoxifying ROS, respectively [30, 97].…”

Section: Discussionmentioning

confidence: 99%

“…Among other keyword-selected putative target genes, B-cell lymphoma 2 ( BCL2 ), catalase ( CAT ), and cAMP responsive element binding protein 1 ( CREB1 ) are reportedly down-regulated in the liver in NAFLD [30, 97, 98]. BCL2 and CAT are major anti-apoptosis genes that function by protecting against mitochondrial outer membrane permeabilization and detoxifying ROS, respectively [30, 97]. Down-regulation of BCL2 and CAT expression in liver tissue drives hepatocyte apoptosis, which is an important pathologic event in the development and progression of NAFLD.…”

Section: Discussionmentioning

confidence: 99%

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

Okamoto

Koda

Okamoto

et al. 2019

Preprint

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1 Serum miR-379 expression is related to the development and progression of 2 hypercholesterolemia in non-alcoholic fatty liver disease 3 4 Short title: Serum miR-379 relates hypercholesterolemia in NAFLD 5 6 Abstract28 Introduction: Non-alcoholic fatty liver disease (NAFLD) has a wide spectrum, 29 eventually leading to cirrhosis and hepatic carcinogenesis. We previously reported that 30 a series of microRNAs (miRNAs) mapped in the 14q32.2 maternally imprinted gene 31 region (Dlk1-Dio3 mat) are related to NAFLD development and progression in a mouse 32 model. We examined the suitability of miR-379, a circulating Dlk1-Dio3 mat miRNA, 33 as a human NAFLD biomarker.34 Methods: Eighty NAFLD patients were recruited for this study. miR-379 was selected 35 from the putative Dlk1-Dio3 mat miRNA cluster because it exhibited the greatest 36 expression difference between NAFLD and non-alcoholic steatohepatitis in our 37 preliminary study. Real-time PCR was used to examine the expression levels of 38 miR-379 and miR-16 as an internal control.39 Results: Compared to normal controls, serum miR-379 expression was significantly 40 up-regulated in NAFLD patients. Receiver operating characteristic curve analysis 41 suggested that miR-379 is a suitable marker for discriminating NAFLD patients from 42 controls, with an area under the curve value of 0.72. Serum miR-379 exhibited positive 43 correlations with alkaline phosphatase, total cholesterol, and low-density-lipoprotein 44 cholesterol levels in patients with early stage NAFLD (Brunt fibrosis stage 0 to 1). The 45 correlation between serum miR-379 and cholesterol levels was lost in early stage 46 NAFLD patients treated with statins. Software-based predictions indicated that various 47 energy metabolism-related genes, including insulin-like growth factor-1 (IGF-1) and48 IGF-1 receptor, are potential targets of miR-379.49 Conclusions: Serum miR-379 exhibits high potential as a biomarker for NAFLD.50 miR-379 appears to increase cholesterol lipotoxicity, leading to the development and 4 51 progression of NAFLD, via interference with the expression of target genes, including 52 those related to the IGF-1 signaling pathway. Our results could facilitate future research 53 into the pathogenesis, diagnosis, and treatment of NAFLD. 54 Introduction 55Non-alcoholic fatty liver disease (NAFLD) is an important cause of chronic liver 56 injury, with an increasing incidence worldwide [1]. NAFLD, regarded as a hepatic 57 manifestation of metabolic syndrome, is defined by significant lipid deposition in 58 hepatocytes (excessive numbers of fat-laden hepatocytes are observed by light 59 microscopy), unrelated to excessive alcohol consumption [2]. The prevalence of 60 NAFLD is almost 25% worldwide and expected to increase with increasing incidence of 61 obesity and metabolic diseases such as type 2 diabetes mellitus (T2DM) and 62 hyperlipidemia [3]. 63The mechanism underlying the development of NAFLD has not been fully 64 elucidated. Currently, the multiple parallel hit theory is the most widely ac...

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“…The pathogenesis of NAFLD involves many mechanisms, such as lipid peroxidation, inflammatory factor damage, oxidative stress and insulin resistance [3,4]. Lipid accumulation and inflammation have been reported to contribute to the development of NAFLD [5,6]. Several studies have also discovered that some compounds can reduce NAFLD by anti-inflammation and inhibition of lipid accumulation, such as alpinetin and rutin [7,8].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Characterizes the Effects and Mechanisms of Quercetin in the Nonalcoholic Fatty Liver Disease Development

Yan¹,

Han²,

Dong³

et al. 2019

Preprint

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As metabolomics is widely used in the study of disease mechanisms, more and more studies have found that metabolites play an important role in the occurrence of diseases. The aim of this study is to investigate the effects and mechanisms of quercetin in high-fat-sucrose diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) development using nontargeted metabolomics. A rat model of NAFLD was established by feeding with a HFD for 30 and 50 days. Results indicated quercetin exhibited hepatoprotective activity in HFD-induced NAFLD rats in 30 days by regulating fatty acids related metabolites (adrenic acid, etc.), inflammation related metabolites (arachidonic acid, etc.), oxidative stress related metabolites (2-hydroxybutyric acid) and other differential metabolites (citric acid, etc.). However, quercetin couldn’t improve NAFLD in 50 days maybe it couldn’t reverse the inflammation condition induced by long-term high-fat diet. These data indicate that dietary quercetin may be beneficial to NAFLD at early stages. Furthermore, combining metabolomics and experimental approaches opens up avenues of effects and mechanisms of drugs for complex diseases.

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The differential effects of high-fat and high fructose diets on the liver of male albino rat and the proposed underlying mechanisms

Cited by 17 publications

References 38 publications

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

Serum miR-379 expression is related to the development and progression of hypercholesterolemia in non-alcoholic fatty liver disease

Metabolomics Characterizes the Effects and Mechanisms of Quercetin in the Nonalcoholic Fatty Liver Disease Development

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