The differential release of eosinophil granule proteins. Studies on patients with acute bacterial and viral infections

Karawajczyk, Malgorzata; Pauksen, Karlis; Peterson, Christer; Eklund, Elisabeth; Venge, Per

doi:10.1111/j.1365-2222.1995.tb00008.x

Cited by 46 publications

(44 citation statements)

References 18 publications

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“…Since EDN levels began to rise coincident with the peak of eosinophilia, it seems likely that the source was the newly recruited eosinophils or immature cells still developing in the bone marrow. Although the differential secretion of EDN and MBP has not been noted previously, there is precedence for the differential secretion of eosinophil granule proteins in bacterial and viral infections (19). EDN levels measured in other parasitic diseases seem to correlate with the number of peripheral blood eosinophils (38).…”

Section: Discussionmentioning

confidence: 97%

Perturbations in Eosinophil Homeostasis following Treatment of Lymphatic Filariasis

Gopinath

Hanna²,

Kumaraswami³

et al. 2000

Infect Immun

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Treatment of patients with patent Wuchereria bancrofti infection results in an acute clinical reaction and peripheral eosinophilia. To investigate the dynamics of the eosinophil response, changes in eosinophil activation and degranulation and plasma levels of eosinophil-active chemokines and cytokines were studied in 15 microfilaremic individuals in south India by sequential blood sampling before and after administration of 300 mg of diethylcarbamazine (DEC). Clinical symptoms occurred within 24 h. Plasma interleukin-5 (IL-5) and RANTES levels peaked 1 to 2 days posttreatment, preceding a peak peripheral eosinophil count at day 4. Major basic protein secretion from eosinophils paralleled IL-5 secretion, while levels of eosinophil-derived neurotoxin peaked at day 13 after treatment. Expression of the activation markers HLA-DR and CD25 on eosinophils rose markedly immediately after treatment, while expression of VLA-4 and ␣4␤7 showed an early peak within 24 h and a second peak at day 13. Thus, the posttreatment reactions seen in filarial infections can be divided into an early phase with killing of microfilariae, clinical symptomatology, increases in plasma IL-5 and RANTES levels, and eosinophil activation and degranulation and a later phase with expression of surface integrins on eosinophils, recruitment of eosinophils from the bone marrow to tissues, and clearance of parasite antigen.

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Section: Discussionmentioning

confidence: 97%

Perturbations in Eosinophil Homeostasis following Treatment of Lymphatic Filariasis

Gopinath

Hanna²,

Kumaraswami³

et al. 2000

Infect Immun

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“…The serum concentration of ECP and EPX were assayed by using radioimmunoassays (Pharmacia Diagnostics AB, Uppsala, Sweden) and of EPO by means of a specific fluorescence enzyme immunoassay (FEIA) (Karawajczyk et al, 1995).…”

Section: Methodsmentioning

confidence: 99%

The eosinophil granule proteins in serum, but not the oxidative metabolism of the blood eosinophils, are increased in cancer

1997

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Summary. The eosinophil activity in patients with renal cell adenocarcinoma during treatment with interleukin-2 (IL-2) and interferon-alpha (IFN-a) was reduced when measured as zymosan-induced lucigenin-enhanced chemiluminescence (CL). Eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and eosinophil protein-X (EPX) were significantly elevated before and during treatment (P < 0 . 001) when compared with the controls. ECP and EPO were unaffected by the treatment whereas it induced an increased EPX level compared with values measured before treatment (P < 0 . 05). The propensity of eosinophils to secrete their granule proteins may reflect the fact that eosinophils in cancer patients have an enhanced capacity to kill cancer cells.

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“…Together with the well-documented involvement of eosinophils in parasitic infection, ECP levels are also increased in viral (Kristjansson et al , 2005 ) and bacterial infection (Venge et al , 1978 ;Karawajczyk et al , 1995 ;Niehaus et al , 2000 ;Ashitani et al , 2003 ), and a specifi c eosinophil activation is reported in bacterial infection (Kvarnhammar and Cardell , 2012 ). Although the direct contribution of eosinophils in bacterial infection is not so well established, many references in the literature indicate that this cell type complements signifi cantly the neutrophil ' s role during host defence.…”

Section: The Eosinophil Cationic Protein (Ecp) In Host Defencementioning

confidence: 96%

Structural determinants of the eosinophil cationic protein antimicrobial activity

Boix

Salazar

Torrent

et al. 2012

Biological Chemistry

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Antimicrobial RNases are small cationic proteins belonging to the vertebrate RNase A superfamily and endowed with a wide range of antipathogen activities. Vertebrate RNases, while sharing the active site architecture, are found to display a variety of noncatalytical biological properties, providing an excellent example of multitask proteins. The antibacterial activity of distant related RNases suggested that the family evolved from an ancestral host-defence function. The review provides a structural insight into antimicrobial RNases, taking as a reference the human RNase 3, also named eosinophil cationic protein (ECP). A particular high binding affi nity against bacterial wall structures mediates the protein action. In particular, the interaction with the lipopolysaccharides at the Gram-negative outer membrane correlates with the protein antimicrobial and specifi c cell agglutinating activity. Although a direct mechanical action at the bacteria wall seems to be suffi cient to trigger bacterial death, a potential intracellular target cannot be discarded. Indeed, the cationic clusters at the protein surface may serve both to interact with nucleic acids and cell surface heterosaccharides. Sequence determinants for ECP activity were screened by prediction tools, proteolysis and peptide synthesis. Docking results are complementing the structural analysis to delineate the protein anchoring sites for anionic targets of biological signifi cance.

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The differential release of eosinophil granule proteins. Studies on patients with acute bacterial and viral infections

Abstract: It is concluded that eosinophils are activated during acute bacterial infections and that this activation results in the preferential mobilisation of ECP. The simultaneous assay of the two eosinophil proteins, ECP and EPO, may give new insight into the role of the eosinophil in disease.

Cited by 46 publications

References 18 publications

Perturbations in Eosinophil Homeostasis following Treatment of Lymphatic Filariasis

Perturbations in Eosinophil Homeostasis following Treatment of Lymphatic Filariasis

The eosinophil granule proteins in serum, but not the oxidative metabolism of the blood eosinophils, are increased in cancer

Structural determinants of the eosinophil cationic protein antimicrobial activity

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