The differentiation of follicular-like cells from the epithelium of Rathke's pouch grown in vitro

Frémont, P.; Ferrand, R

doi:10.1007/bf00305108

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…In ''nondifferentiating'' conditions, a few hormone-producing cells appeared at the center of the sphere, whereas one might expect them on the outside (if ''outside'' corresponded to ''away from'' the MCs). However, a previous study with quail RPs in culture also showed differentiation in the center, with cells lining the lumen of the pouch migrating toward the outside of the explant (29). It was notable that when growth factors that prevent differentiation were absent from the pituisphere medium, the extensive differentiation that occurred was now mostly at the surface.…”

Section: Discussionmentioning

confidence: 86%

“…After 4 days, pituispheres were found at a density of 1 per 5,000 to 1 per 10,000 cells seeded. The number of cells per pituisphere was 22.7 Ϯ 0.9 (mean Ϯ SEM of 20 spheres; range, [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. When cultured in the presence of BrdU for 24 h after the first day of culture, virtually all cells were labeled by day 4, suggesting growth by cell division rather than reaggregation (Fig.…”

Section: Sox9 Expression In the Pituitary Marks Differentiating Progementioning

confidence: 99%

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SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

Fauquier¹,

Rizzoti

Dattani³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

342

447

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The pituitary gland adapts the proportion of each of its endocrine cell types to meet differing hormonal demands throughout life. There is circumstantial evidence that multipotent adult progenitor cells contribute to this plasticity, but these cells have not been identified. Here, we describe a small (<0.05%) population of progenitor cells in the adult pituitary gland. We show that these cells express SOX2, a marker of several early embryonic progenitor and stem cell types, and form ''pituispheres'' in culture, which can grow, form secondary spheres, and differentiate to all of the pituitary endocrine cell types, as well as folliculostellate cells. Differentiation of cells in the pituispheres was associated with the expression of nestin, SOX9, and S100. Cells expressing SOX2 and E-cadherin are found throughout Rathke's pouch (RP) in embryos but persist in the adult gland, mostly in a narrow zone lining the pituitary cleft, but also are scattered throughout the pituitary. However, unlike in embryonic RP, most of these SOX2 ؉ cells in the adult gland also express SOX9 and S100. We suggest that this SOX2 ؉ /SOX9 ؉ population represents transit-amplifying cells, whereas the SOX2 ؉ /SOX9 ؊ cells we identify are multipotent progenitor/stem cells persisting in the adult pituitary.stem cell ͉ folliculostellate cells

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Section: Discussionmentioning

confidence: 86%

Section: Sox9 Expression In the Pituitary Marks Differentiating Progementioning

confidence: 99%

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

Fauquier¹,

Rizzoti

Dattani³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

342

447

View full text Add to dashboard Cite

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“…These cytoplasmic processes faced the basal lamina (Fig. 13) Fremont and Ferrand, 1981;Soji, 1984). Some have suggested that it was a stem cell which eventually transformed into a hormone-secreting cell (Rennels, 1964;Kurosumi and Kobayashi, 1968;Yamashita, 1972;Yoshimura et al, 1977).…”

Section: Conventional Electron Microscopymentioning

confidence: 97%

Intercellular communication between rat anterior pituitary cells

Soji

Herbert

1989

Anat. Rec.

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Cell-to-cell communication within the rat anterior pituitary was investigated in 60-day-old male rats with immunohistochemistry, scanning electron microscopy, freeze-fracture electron microscopy, and conventional transmission electron microscopy. A dense cytoreticular network of cytoplasmic processes from the folliculostellate cells was found to contain immunoreactive S-100 protein and was observed throughout the anterior pituitary. Nonimmunoreactive cells, which were granular, were situated in the center of each network. Almost all of the granulated cells were situated in close proximity to the folliculostellate cells. Scanning electron microscopy revealed that the gland consisted of microlobules enclosed by a basal lamina. On the surface of the microlobules were blood vessels whose branches invaded its internal structures. Cytoplasmic processes from folliculostellate cells projected outside the microlobule. Freeze-fracture electron microscopy demonstrated the presence of numerous intramembranous particles on the P-face of the plasma membrane. Scattered on the cell surface were groups of particles forming gap junctions. Meshworks of ridges which were representations of tight junctions were also observed near clusters of microvillous fragments. Clusters of particles forming small gap junctions were located between the meshworks of tight junctions. Small gap junctions were clearly observed by conventional electron microscopy between junctional complexes in a manner similar to that seen by freeze-fracture electron microscopy. Slender cytoplasmic processes of folliculostellate cells came in contact near the basal lamina and were adjoined by small gap junctions. The ratio of nongranular cells which contained gap junctions to those in which the junctions were absent was about 1:1. The size of the gap junctions ranged from 50 nm to 3 microns. No gap junctions were observed along the plasma membranes of the granular cells. The significance of an intercellular communication system within the anterior pituitary gland of the rat is to establish a mechanism for rapid transmission of information in an organ which lacks direct innervation.

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“…A number of transcription factors and cofactors regulating the lineage commitment and terminal differentiation of distinct cell types are illustrated in a genetic pathway. [Modified from Scully and Rosenfeld (255) ] pituitary (80,81,83,84,301), but also can induce the differentiation of the anterior pituitary cell types in naive head ectoderm of early chick embryo, such as lateral head ectoderm, presumptive lens, and face ectoderm, as has been demonstrated by in vitro organ culture experiments (78,79,92). The process of induction is strictly contactdependent and also requires the presence of mesenchyme.…”

Section: B Initial Steps Of Rathke's Pouch Developmentmentioning

confidence: 99%

Molecular Physiology of Pituitary Development: Signaling and Transcriptional Networks

Zhu

Gleiberman²,

Rosenfeld³

2007

Physiological Reviews

323

296

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The pituitary gland is a central endocrine organ regulating basic physiological functions, including growth, the stress response, reproduction, metabolic homeostasis, and lactation. Distinct hormone-producing cell types in the anterior pituitary arise from a common ectodermal primordium during development by extrinsic and intrinsic mechanisms, providing a powerful model system for elucidating general principles in mammalian organogenesis. The central purpose of this review is to inspect the integrated signaling and transcriptional events that affect precursor proliferation, cell lineage commitment, terminal differentiation, and physiological regulation by hypothalamic tropic factors.

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The differentiation of follicular-like cells from the epithelium of Rathke's pouch grown in vitro

Cited by 11 publications

References 12 publications

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

Intercellular communication between rat anterior pituitary cells

Molecular Physiology of Pituitary Development: Signaling and Transcriptional Networks

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