The Diffusion of Dyes Through Articular Cartilage in Vivo

Brower, Thomas D.; Akahoshi, Yoshihiko; Orlic, Patricia

doi:10.2106/00004623-196244030-00002

Cited by 42 publications

(17 citation statements)

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“…However, although no work has been done on this point, it seems reasonable to believe that the two regions may differ in the efficiency of their nutrition, the central region of costal cartilage being the poorer in this respect. At least in terms of distance, the cells of the latter are further away from a nutrient fluid, in this case the perichondrial capillary blood, than are all except perhaps the deepest cells of articular cartilage, assuming that the synovial fluid forms the principal if not the sole source of nutrition of this tissue (Brower, Akahoshi, and Orlic, 1962). It is possible on theoretical grounds that the availability of diphosphopyridine nucleotide (Coenzyme I) in the oxidized form could influence the relative proportions of hexose and hexuronic acid in any given population of polysaccharide molecules.…”

Section: Annals Of the Rheumatic Diseasesmentioning

confidence: 99%

Observations on the acid glycosaminoglycan (mucopolysaccharide) content of the matrix of aging cartilage.

Stockwell¹,

Scott²

1965

Annals of the Rheumatic Diseases

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The changes which occur with age in joint cartilage (Davies, 1961) and ordinary hyaline cartilage (Silberberg and Silberberg, 1961) (Bennett, Waine, and Bauer, 1942), information about keratosulphate should be of interest in the elucidation of the aetiology ofjoint disease. To this end articular as well as ordinary hyaline cartilage have been examined and compared. Material and Methods MaterialHuman post-mortem material was used. Specimens of the 4th costal cartilage and of articular cartilage from the medial femoral condyle were taken; areas free of macroscopic erosions were sampled. The ages of the subjects were 1 , 13k, 18, 31, 34, 60, 64, 69, and 78 years. In addition, a specimen of articular cartilage from the head of the humerus of an amputated limb from a person aged 50 years was also examined. MethodsBlocks were fixed for 3 days at -20°C. in alcoholic formalin (1 part 40 per cent. formaldehyde: 9 parts ethanol), carried through two changes, each for 24 hrs, of absolute alcohol, and of xylol (at -200 C.). The final change in xylol was allowed to warm to room temperature, before routine embedding in paraffin wax, and sectioning at lO,u. Sections were dewaxed with xylol and allowed to dry on the slide at room temperature before staining. Sections were stained as set out below:

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Section: Annals Of the Rheumatic Diseasesmentioning

confidence: 99%

Observations on the acid glycosaminoglycan (mucopolysaccharide) content of the matrix of aging cartilage.

Stockwell¹,

Scott²

1965

Annals of the Rheumatic Diseases

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“…Various studies have been made into the diffusive permeability of certain connective tissues such as intervertebral disc (Paulson et al, 1951), articular cartilage (Kantor & Schubert, 1957;Brower et al, 1962;Gerber & Schubert, 1964;Maroudas, 1970;Lotke & Granda, 1972) and cornea (Hedbys, 1963). The difficulties that present themselves in studying Vol.…”

mentioning

confidence: 99%

Permeability of composite chondrocyte-culture-millipore membranes to solutes of varying size and shape

Cumming¹,

Handley²,

Preston³

1979

Biochemical Journal

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A model connective-tissue system was developed that is amenable to the determination of permeability coefficients of diffusing solutes. The system involves the culture of 13-day chick-embryo chondrocytes on a Millipore filter (HA: 0.454um pore size) to form what is, in effect, a confluent, extremely thin cartilage slice of uniform thickness. These cultured chondrocyte membranes were used to measure the steady-state flux of radioactively labelled low-molecular-weight solutes and micro-ions. Similarly, the permeability coefficients of either radioactively labelled or enzymically active proteins across the membranes were determined. The membrane was found to have no marked effects on the diffusional behaviour of low-molecular-weight non-electrolytes (water, proline, ribose, glucose, sorbitol, raffinose). For micro-ions (Na+, S042-, Cl-, glutamate, glucuronate), the diffusive behaviour was found to be markedly affected by the ionic strength of the solvent used in a manner which was consistent with a Donnan distribution resulting from the immobilized proteoglycans. Globular proteins permeated the membrane at rates which decreased as the molecular size of the diffusing solute increased. The apparent diffusion rates of fibrinogen and of collagen through the membranes were greater than would be expected on the basis of their diffusion coefficients in free solution. Reasons for this behaviour are discussed.

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“…This is likely to be due both to decreased perfusion pressure through cartilage and to the decreased permeability demonstrated, in cartilage, within 10 min. of death (Brower, Akahoshi, and Orlic, 1962). This finding might suggest that, in addition to the well-established role of synovial fluid in cartilage nutrition, perfusion of cartilage from underlying vessels also plays a role in cartilage metabolism.…”

Section: Discussionmentioning

confidence: 92%

Living articular cartilage is not smooth. The structure of mammalian and avian joint surfaces demonstrated in vivo by immersion incident light microscopy.

Garner¹,

McGillivray²

1971

Annals of the Rheumatic Diseases

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The Diffusion of Dyes Through Articular Cartilage in Vivo

Cited by 42 publications

References 0 publications

Observations on the acid glycosaminoglycan (mucopolysaccharide) content of the matrix of aging cartilage.

Observations on the acid glycosaminoglycan (mucopolysaccharide) content of the matrix of aging cartilage.

Permeability of composite chondrocyte-culture-millipore membranes to solutes of varying size and shape

Living articular cartilage is not smooth. The structure of mammalian and avian joint surfaces demonstrated in vivo by immersion incident light microscopy.

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