Digestibility and balance trials were performed with six growing pigs, to evaluate the nutritive value and protein utilization of Eurolysine bacterial protein and Pekilo protein used to replace 50 % or 100 % of soybean supplement in a barley-based diet. Eurolysine had a high content of crude protein, 67.8 % of DM, and was rich in lysine, 8.3 g/16 N, and the corresponding values for Pekilo were 49.5 % and 5,5 g/16 g N. The diets containing Eurolysine had lower digestibilities than those with Pekilo or soybean meal. With Eurolysine the digestibility values for crude protein, ether extract and N-free extract (NFE) were, respectively, 64.3 %, 35.4 % and 76.5 %, and with Pekilo 74.8 %, 28.5 % and 79.4 %. The feed values for Eurolysine were 0.82 FU/kg, 509 g DCP/kg and 12.0 MJ ME/kg DM, and those for Pekilo 0.90 FU/kg, 409 g DCP/kg and 12.6 MJ ME/kg DM. The urinary nitrogen excretion was lower with Eurolysine than with the other supplements, and the nitrogen retention and the biological values were higher (P < 0.05). Eurolysine is well suited as a protein supplement in pig feeding because of its high content of lysine. Combined with barley the protein in Eurolysine gave a high biological value.