The Digestive Function in Developing Fish Larvae and Fry. From Molecular Gene Expression to Enzymatic Activity

Yúfera, Manuel; Moyano, Francisco Javier; Martı́nez-Rodrı́guez, Gonzalo

doi:10.1007/978-3-319-73244-2_3

Cited by 35 publications

(26 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduction in amylase activity in RT and CBT with age may be linked to carnivorous feeding as previously reported for other carnivorous fish species such as seabream and other marine species (Khoa et al, 2019;Yúfera et al, 2018). No progressive increase in amylase was observed in the triploid hybrids during early development, suggesting a difference in the carbohydrate metabolism pattern in this particular group compared with RT and CBT.…”

Section: Discussionsupporting

confidence: 76%

Comparative ontogeny of the digestive tract ofOncorhynchus mykiss♀ xSalmo trutta caspius♂ triploid hybrids to their parental species

et al. 2020

View full text Add to dashboard Cite

Hybridization or the crossing of individuals from the same species (crossbreeding) or different species (interspecific) offers the possibility of improving traits that benefit aquaculture. Hybridization in salmonids has been used to improve disease resistance, growth rates, manipulate sex ratios and to produce sterile fish (Bartley et al., 2000). For example, Salmo trutta x Salvelinus fontinalis hybrids are sterile (Scheerer & Thorgaard, 1983). The hybrids of Salvelinus namaycush x S. fontinalis (maintained at low pH, 5.5-7.2) and Salmo labrax x Oncorhynchus mykiss have increased growth rates relative to the parental species by day 200 post-hatch (Akhan et al., 2011), while O. mykiss x Salvelinus sp. and S. trutta x S. salar hybrids have increased disease resistance (Maynard et al., 2016). Despite the potential advantages of the salmonid hybrids, their exploitation for aquaculture is uncommon due to high mortality during embryonic stages and up until external feeding (Bartley et al., 2000). Nonetheless, there are some reports that triploidization can increase the viability of salmonid hybrids (Blanc & Maunas, 2005; Blanc et al., 2000; Scheerer & Thorgaard, 1983). One potential reason for high mortalities during the larval stage may be abnormalities in digestive tract function and for this reason characterization of gastrointestinal tract development and the nutritional physiology of fish larvae can contribute to overcome such problems (Rønnestad et al., 2013). Caspian brown trout, CBT, Salmo trutta caspius is one of the native cold water species with high potential for aquaculture in the

show abstract

Section: Discussionsupporting

confidence: 76%

Comparative ontogeny of the digestive tract ofOncorhynchus mykiss♀ xSalmo trutta caspius♂ triploid hybrids to their parental species

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The ontogeny of digestive system has been traditionally addressed using anatomical, histological, and biochemical approaches. These methods have been complemented more recently with molecular biology techniques [5,6]. Although the study of the ontogeny of digestive enzymes at transcriptional level can provide useful information due to its high accuracy and specificity even for different gene isoforms [7], it has been carried out in relatively few species [6].…”

Section: Introductionmentioning

confidence: 99%

“…These methods have been complemented more recently with molecular biology techniques [5,6]. Although the study of the ontogeny of digestive enzymes at transcriptional level can provide useful information due to its high accuracy and specificity even for different gene isoforms [7], it has been carried out in relatively few species [6]. Additionally, even fewer studies have assessed the ontogeny of digestive system using simultaneously molecular and biochemical techniques [5,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, even fewer studies have assessed the ontogeny of digestive system using simultaneously molecular and biochemical techniques [5,8,9]. Such studies can provide a better insight into transcriptional/translational relations of the digestive machinery and can help to understand to what extent the enzymatic hydrolysis of macronutrients is programmed, activated, and/or modulated [6].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of present work was to evaluate the early ontogeny of key digestive enzymes and somatotropic factors at biochemical and/or transcriptional levels during the initial development of the thick-lipped grey mullet. Unlike most of the available literature, in which only short periods of 30-45 dph are usually addressed [6], this work encompasses a wider period, from hatching until three months of life. To evaluate the digestive functionality, the activity of several key digestive enzymes involved in the digestion of proteins, lipids and carbohydrates (trypsin, chymotrypsin, pepsin, lipase, and amylase) were measured.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

Gilannejad

Heras

Martos-Sitcha

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of key digestive enzymes was measured from three until 90 days post hatch (dph). Expression of genes involved in digestion of proteins (try2, ctr, pga2, and atp4a), carbohydrates (amy2a), and lipids (cel and pla2g1b), together with two somatotropic factors (gh and igf1) were also quantified. No chymotrypsin or pepsin activities were detected. While specific activity of trypsin and lipase were high during the first 30 dph and declined afterward, amylase activity was low until 57 dph and increased significantly beyond that point. Expression of try2, ctr, amy2a, and cel increased continuously along development, and showed a peak at the end of metamorphosis. Expression of pla2g1b, pga2 and atp4a increased until the middle of metamorphosis and decreased afterwars. Most of these trends contrast the usual patterns in carnivorous species and highlight the transition from larvae, with high protein requirements, to post-larvae/juvenile stages, with omnivorous/detritivorous feeding preferences. Somatotropic genes, gh and igf1, showed approximately inverse expression patterns, suggesting the establishment of the Gh/Igf1 axis from 50 dph.

show abstract

Molecular basis of the digestive functionality in developing Persian sturgeon (Acipenser persicus) larvae: additional clues for its phylogenetic status

et al. 2019

View full text Add to dashboard Cite

The Digestive Function in Developing Fish Larvae and Fry. From Molecular Gene Expression to Enzymatic Activity

Cited by 35 publications

References 120 publications

Comparative ontogeny of the digestive tract ofOncorhynchus mykiss♀ xSalmo trutta caspius♂ triploid hybrids to their parental species

Comparative ontogeny of the digestive tract ofOncorhynchus mykiss♀ xSalmo trutta caspius♂ triploid hybrids to their parental species

Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

Molecular basis of the digestive functionality in developing Persian sturgeon (Acipenser persicus) larvae: additional clues for its phylogenetic status

Contact Info

Product

Resources

About