The digestive tract sections of the sea cucumber Isostichopus badionotus reveal differences in composition, diversity, and functionality of the gut microbiota

Quintanilla-Mena, Mercedes; Olvera‐Novoa, Miguel A.; Sánchez‐Tapia, Itzel A.; Lara-Pérez, Luis A.; Rivas-Reyes, Isajav; Klanian, Mariel Gullian; Patiño-Suárez, María V; Puch-Hau, Carlos

doi:10.1007/s00203-022-03080-9

Cited by 4 publications

(4 citation statements)

References 78 publications

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“…The intestinal microbiota composition of early SUS I. badionotus juveniles was mainly composed of Proteobacteria (50.2%), Bacteroidetes (25.7%), Campylobacterota (12.3%) and Actinobacteria (3.1%), with Firmicutes present at a lower relative abundance of 2.8%. These results were similar at the Phylum level to those found in sea cucumbers of the same species kept in captivity (organisms collected from wild populations and kept in fiberglass tanks for six months) [12]. These authors reported that Proteobacteria (60.9%), Actinobacteria (11.6%), and Bacteroidetes (6.8%) were the predominant bacterial Phyla in the intestine.…”

Section: Plos Onesupporting

confidence: 80%

“…However, a decrease in the abundance of Vibrio can have significant consequences because it allows for the colonization and proliferation of potentially pathogenic and opportunistic bacteria, such as Halarcobacter (syn. Arcobacter), that has also been identified as part of the microbiota of healthy sea cucumber organisms [12,58].…”

Section: Plos Onementioning

confidence: 99%

“…However, the research efforts have primarily focused on aquaculture technology, nutrition, and feeding, as well as its nutraceutical or pharmacological potential [6][7][8][9][10][11]. Recently, this species' gut microbiota composition in captivity was reported using high-throughput 16S rRNA sequencing [12].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of microbiota and histology of cultured sea cucumber Isostichopus badionotus juveniles during an outbreak of skin ulceration syndrome

Arjona-Cambranes,

Olvera-Novoa,

Cerqueda-García

et al. 2024

PLoS ONE

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Due to the dramatic reduction of sea cucumber Isostichopus badionotus populations in the Yucatan Peninsula by overfishing and poaching, aquaculture has been encouraged as an alternative to commercial catching and restoring wild populations. However, the scarcity of broodstock, the emergence of a new disease in the auricularia larvae stage, and the development of skin ulceration syndrome (SUS) in the culture have limited aquaculture development. This study presents the changes in the intestine and skin microbiota observed in early and advanced stages of SUS disease in cultured juvenile I. badionotus obtained during an outbreak in experimental culture through 16S rRNA gene sequencing and histological evidence. Our results showed inflammation in the intestines of juveniles at both stages of SUS. However, more severe tissue damage and the presence of bacterial clusters were detected only in the advanced stages of SUS. Differences in the composition and structure of the intestinal and skin bacterial community from early and advanced stages of SUS were detected, with more evident changes in the intestinal microbial communities. These findings suggest that SUS was not induced by a single pathogenic bacterium. Nevertheless, a decrease in the abundance of Vibrio and an increase in Halarcobacter (syn. Arcobacter) was observed, suggesting that these two bacterial groups could be keystone genera involved in SUS disease.

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Section: Plos Onesupporting

confidence: 80%

Section: Plos Onementioning

confidence: 99%

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Characterization of microbiota and histology of cultured sea cucumber Isostichopus badionotus juveniles during an outbreak of skin ulceration syndrome

Arjona-Cambranes,

Olvera-Novoa,

Cerqueda-García

et al. 2024

PLoS ONE

Self Cite

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“…This trend corresponds to the digestibility of cellulose and hemicellulose that we measured, implying that bacterial diversity influences their digestibility in L. migratoria . The consistent presence of Enterobacter bacteria in the hindgut has been repeatedly confirmed, indicating a selective interaction between locusts and these bacteria ( 48 , 61 , 62 ). Shi et al ( 63 ) reported that an entomopathogenic fungus almost entirely inhibited the growth of isolated Enterobacte r bacteria in the hindgut by lowering the pH from 6.3 to 5.6, suggesting that the hindgut is characteried by specific conditions conducive to the growth of certain intestinal bacteria, thus further confirming their adaptive growth in the gut and the close symbiotic relationship between locusts and specific bacterial species, such as species of Enterobacter .…”

Section: Discussionmentioning

confidence: 74%

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Li,

Liang

et al. 2024

mSystems

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Locusta migratoria is an important phytophagous pest, and its gut microbial communities play an important role in cellulose degradation. In this study, the gut microbial and cellulose digestibility dynamics of Locusta migratoria were jointly analyzed using high-throughput sequencing and anthrone colorimetry. The results showed that the gut microbial diversity and cellulose digestibility across life stages were dynamically changing. The species richness of gut bacteria was significantly higher in eggs than in larvae and imago, the species richness and cellulose digestibility of gut bacteria were significantly higher in early larvae (first and second instars) than in late larvae (third to fifth instars), and the diversity of gut bacteria and cellulose digestibility were significantly higher in imago than in late larvae. There is a correlation between the dynamics of gut bacterial communities and cellulose digestibility. Enterobacter , Lactococcus, and Pseudomonas are the most abundant genera throughout all life stages. Six strains of highly efficient cellulolytic bacteria were screened, which were dominant gut bacteria. Carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) experiments revealed that Pseudomonas had the highest cellulase enzyme activity. This study provides a new way for the screening of cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors. IMPORTANCE Cellulose is the most abundant and cheapest renewable resource in nature, but its degradation is difficult, so finding efficient cellulose degradation methods is an urgent challenge. Locusta migratoria is a large group of agricultural pests, and the large number of microorganisms that inhabit their intestinal tracts play an important role in cellulose degradation. We analyzed the dynamics of Locusta migratoria gut microbial communities and cellulose digestibility using a combination of high-throughput sequencing technology and anthrone colorimetry. The results revealed that the gut microbial diversity and cellulose digestibility were dynamically changed at different life stages. In addition, we explored the intestinal bacterial community of Locusta migratoria across life stages and its correlation with cellulose digestibility. The dominant bacterial genera at different life stages of Locusta migratoria were uncovered and their carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) were determined. This study provides a new avenue for screening cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors.

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Effects of dietary Bacillus cereus supplementation on the growth performance, serum physiology and biochemistry, Nrf2, TLR/NF-κB signaling pathways, and intestinal health of juvenile coho salmon (Oncorhynchus kisutch)

Li,

Xie,

Guo

et al. 2024

Aquaculture Reports

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The digestive tract sections of the sea cucumber Isostichopus badionotus reveal differences in composition, diversity, and functionality of the gut microbiota

Cited by 4 publications

References 78 publications

Characterization of microbiota and histology of cultured sea cucumber Isostichopus badionotus juveniles during an outbreak of skin ulceration syndrome

Characterization of microbiota and histology of cultured sea cucumber Isostichopus badionotus juveniles during an outbreak of skin ulceration syndrome

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Effects of dietary Bacillus cereus supplementation on the growth performance, serum physiology and biochemistry, Nrf2, TLR/NF-κB signaling pathways, and intestinal health of juvenile coho salmon (Oncorhynchus kisutch)

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