2000
DOI: 10.1034/j.1600-0854.2000.010807.x
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The Dileucine Motif Within the Tail of MPR46 is Required for Sorting of the Receptor in Endosomes

Abstract: The cytoplasmic tail of MPR46 carries a leucine-based motif that is required for the sorting of lysosomal enzymes by the receptor. In addition, it is one of three independent, but functionally redundant, internalization signals present in the cytoplasmic tail of MPR46. We have analyzed a mutant of MPR46, in which the dileucine pair was replaced by alanines (MPR46 LL/AA) with respect to its intracellular distribution and trafficking. Ultrastructural analysis of cells expressing the MPR46 LL/AA mutant revealed t… Show more

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Cited by 54 publications
(35 citation statements)
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“…A second pathway by which proteins are delivered directly from early or recycling endosomes to the TGN (29,30) can also be used by MPR46. The LL motif in the MPR46 tail is involved in this pathway, probably through interaction with AP-1 complexes (54). Interestingly, the return of an LL/AA mutant of MPR46 from the plasma membrane or endosome pool to the TGN was impaired but not abolished, while recycling from the endosomes to the plasma membrane was enhanced (54).…”
Section: Discussionmentioning
confidence: 99%
“…A second pathway by which proteins are delivered directly from early or recycling endosomes to the TGN (29,30) can also be used by MPR46. The LL motif in the MPR46 tail is involved in this pathway, probably through interaction with AP-1 complexes (54). Interestingly, the return of an LL/AA mutant of MPR46 from the plasma membrane or endosome pool to the TGN was impaired but not abolished, while recycling from the endosomes to the plasma membrane was enhanced (54).…”
Section: Discussionmentioning
confidence: 99%
“…Two types of dileucinebased sorting signals have been identified in C-terminal cytoplasmic domains of several membrane proteins so far. The DXXLL motif present in the cytoplasmic tails of the mannose 6-phosphate receptors is essential for sorting in the TGN and endosomes and is recognized by the cytosolic monomeric Golgilocalized, ␥-ear-containing, ARF-binding proteins (GGA) (48,55). A second dileucine motif, (DE)XXXL(LI), is critical for targeting transmembrane proteins, such as LIMP-2, tyrosin- ase, TRP-1, and QNR71, to lysosomes and lysosome-related organelles (22,23,56,57).…”
Section: Discussionmentioning
confidence: 99%
“…Mark McNiven, Martin Lowe, and Anna Shestakova. Antibodies against Crn7 and Crn3 (monoclonal) and MPR46 and Erd2p (polyclonal) were reported previously (9,(12)(13)(14). Monoclonal antibodies against ␥-adaptin were from Sigma and against Src from Calbiochem, polyclonal antibodies against ␣-adaptin and against AP-3 were from Santa Cruz Biotechnology.…”
Section: Methodsmentioning
confidence: 99%