The direct and indirect transformation methods on expressing a recombinant Dermaseptin peptide in tobacco transgenic hairy root clones

Nazarian-Firouzabadi, Farhad; Nazarian-Firouzabadi, Farhad; Ismaili, Ahmad

doi:10.1016/j.cpb.2020.100177

Cited by 9 publications

(3 citation statements)

References 34 publications

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“…108 The use of A. rhizogenes as a gene vector for genetic engineering in higher plants has been widely documented. 109 A tobacco hairy root expression system has been used to produce recombinant bovine lactoferrin-derived antimicrobial peptide, 107 dermaseptin peptide, 110 stilbene compounds 111 and other substances. In 2004, Taura et al 72 reported the use of tobacco hairy roots for the heterologous production of THC using tobacco hairy roots, by expressing THCAS and exogenously feeding CBGA.…”

Section: Application Of Hairy Roots In Cannabinoidmentioning

confidence: 99%

Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)

Wang,

Zang,

Zhao

et al. 2024

ACS Synth. Biol.

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Cannabis sativa L. is a multipurpose crop with high value for food, textiles, and other industries. Its secondary metabolites, including cannabidiol (CBD), have potential for broad application in medicine. With the CBD market expanding, traditional production may not be sufficient. Here we review the potential for the production of CBD using biotechnology. We describe the chemical and biological synthesis of cannabinoids, the associated enzymes, and the application of metabolic engineering, synthetic biology, and heterologous expression to increasing production of CBD.

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Section: Application Of Hairy Roots In Cannabinoidmentioning

confidence: 99%

Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)

Wang,

Zang,

Zhao

et al. 2024

ACS Synth. Biol.

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“…The total recombinant protein content of transgenic clones produced using direct and indirect transformation techniques and in liquid medium differs noticeably. According to the study, recombinant peptide production from the hairy root derived from the indirect transformation approach was noticeably greater [ 186 ]. Verticillium dahlia , an economically significant plant pathogen, is very resistant to the antimicrobial peptide BTD-S. Plants of the wild-type Columbia-0 ecotype of Arabidopsis thaliana expressed this peptide.…”

Section: Antimicrobial Peptide Production In Plants—prospects and Adv...mentioning

confidence: 99%

Antimicrobial Peptides: The Production of Novel Peptide-Based Therapeutics in Plant Systems

Tiwari,

Srivastava,

Sharma

et al. 2023

Life

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The increased prevalence of antibiotic resistance is alarming and has a significant impact on the economies of emerging and underdeveloped nations. The redundancy of antibiotic discovery platforms (ADPs) and injudicious use of conventional antibiotics has severely impacted millions, across the globe. Potent antimicrobials from biological sources have been extensively explored as a ray of hope to counter the growing menace of antibiotic resistance in the population. Antimicrobial peptides (AMPs) are gaining momentum as powerful antimicrobial therapies to combat drug-resistant bacterial strains. The tremendous therapeutic potential of natural and synthesized AMPs as novel and potent antimicrobials is highlighted by their unique mode of action, as exemplified by multiple research initiatives. Recent advances and developments in antimicrobial discovery and research have increased our understanding of the structure, characteristics, and function of AMPs; nevertheless, knowledge gaps still need to be addressed before these therapeutic options can be fully exploited. This thematic article provides a comprehensive insight into the potential of AMPs as potent arsenals to counter drug-resistant pathogens, a historical overview and recent advances, and their efficient production in plants, defining novel upcoming trends in drug discovery and research. The advances in synthetic biology and plant-based expression systems for AMP production have defined new paradigms in the efficient production of potent antimicrobials in plant systems, a prospective approach to countering drug-resistant pathogens.

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“…When producing recombinant proteins from a plant expression system, there are numerous approaches available with a difference in yield, ease of harvest, and PTMs to suit each specific recombinant protein. Genetically modifying plant genomes to create transgenic plants is a conventional method for the production of recombinant proteins [57,58]. However, this method requires long time-consuming processes that are labor-intensive and non-flexible in dealing with SARS-CoV-2 variations that vary over time [59].…”

Section: Transient Expressionmentioning

confidence: 99%

Development of surrogate SARS-Cov-2 virus neutralization test method using plant-produced recombinant proteins

Jirarojwattana

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Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but a conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and a pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals in BSL2. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme (ACE2) produced from�Nicotiana benthamiana�for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2-His can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with plant-produced RBD protein. The sVNT developed using plant-produced proteins showed high sensitivity and specificity when validated with a group of 30 RBD-Fc vaccinated mice sera and the results were correlated with cVNT titer. This preliminary finding suggests that the plants could offer a cost-effective platform for establishing antibody detection assays.

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The direct and indirect transformation methods on expressing a recombinant Dermaseptin peptide in tobacco transgenic hairy root clones

Cited by 9 publications

References 34 publications

Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)

Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)

Antimicrobial Peptides: The Production of Novel Peptide-Based Therapeutics in Plant Systems

Development of surrogate SARS-Cov-2 virus neutralization test method using plant-produced recombinant proteins

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