Objective: at the assessment of bacteriostatic properties of the thin-film coating prototypes meant for orthopedic titanium and titanium alloys implants. Material and methods. The morphology of the Ti-6AL-4V, ASTM F1472 samples with the 50-70 nm thin-film CuO coating deposited on their surfaces with microarc oxidation was examined by scanning electron microscopy. Then we evaluated the effects of the thin-film prototypes on the clinical strains ability to adhere, form biofilms and their growth properties. Results. The newly designed prototype causes the significant decrease in the mass of biofilms pre-formed by the clinical strains (Staphylococcus aureus by 11%, Staphylococcus epidermidis by 38%, and Pseudomonas aeruginosa by 7%) and inhibits their growth properties (S. aureus by 12.7%, S. epidermidis by 13.3%, and P. aeruginosa by 10%). Conclusion. This thin-film coating prototype on the surfaces of the titanium and titanium alloys implants decreases pathogenic factors in the microorganism clinical strains due to its pronounced bacteriostatic effect, slowdown in adhesive activity and inhibition of their ability to form biofilms.