The discovery and identification of a candidate proteomic biomarker of active tuberculosis

Liu, Jiyan; Wei, Li-Liang; Yang, Xinying; Wang, Chong; Zhang, Xing; Xu, Dandan; Chen, Zhongliang; Yang, Fuquan; Li, Jicheng

doi:10.1186/1471-2334-13-506

Cited by 41 publications

(36 citation statements)

References 32 publications

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“…Notably, a series of changes in the level of serum proteins are caused by Mtb infection [9,10]. Therefore, serum proteins may serve as ideal biomarkers for the diagnosis of TB.…”

Section: Introductionmentioning

confidence: 99%

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Shi

Wei

et al. 2017

PLoS ONE

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The aim of this study was to discover novel biomarkers for pulmonary tuberculosis (TB). Differentially expressed proteins in the serum of patients with TB were screened and identified by iTRAQ-two dimensional liquid chromatography tandem mass spectrometry analysis. A total of 79 abnormal proteins were discovered in patients with TB compared with healthy controls. Of these, significant differences were observed in 47 abnormally expressed proteins between patients with TB or pneumonia and chronic obstructive pulmonary disease (COPD). Patients with TB (n = 136) exhibited significantly higher levels of serum amyloid A (SAA), vitamin K-dependent protein Z (PROZ), and C4b-binding protein β chain (C4BPB) than those in healthy controls (n = 66) (P<0.0001 for each) albeit significantly lower levels compared with those in patients with pneumonia (n = 72) (P<0.0001 for each) or COPD (n = 72) (P<0.0001, P<0.0001, P = 0.0016, respectively). After 6 months of treatment, the levels of SAA and PROZ were significantly increased (P = 0.022, P<0.0001, respectively), whereas the level of C4BPB was significantly decreased (P = 0.0038) in treated TB cases (n = 72). Clinical analysis showed that there were significant differences in blood clotting and lipid indices in patients with TB compared with healthy controls, patients with pneumonia or COPD, and treated TB cases (P<0.05). Correlation analysis revealed significant correlations between PROZ and INR (rs = 0.414, P = 0.044), and between C4BPB and FIB (rs = 0.617, P = 0.0002) in patients with TB. Receiver operating characteristic curve analysis revealed that the area under the curve value of the diagnostic model combining SAA, PROZ, and C4BPB to discriminate the TB group from the healthy control, pneumonia, COPD, and cured TB groups was 0.972, 0.928, 0.957, and 0.969, respectively. Together, these results suggested that SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB. Our study may thus provide experimental data for the differential diagnosis of TB.

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“…Notably, a series of changes in the level of serum proteins are caused by Mtb infection [9,10]. Therefore, serum proteins may serve as ideal biomarkers for the diagnosis of TB.…”

Section: Introductionmentioning

confidence: 99%

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Shi

Wei

et al. 2017

PLoS ONE

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“…As such, the following methods are currently being used to search fornew TB biomarkers: cytokine array analyses of the sera from patients with active or latent TB infections compared to those from healthy individuals (Weiner et al, 2012;Liu et al, 2013); microarray analyses of the supernatants of centrifuged peripheral blood after stimulation with TB-specific antigens from patients with active or latent TB infections compared to that from healthy individuals (Frahm et al, 2011;Kellar et al, 2011;Murthy et al, 2011;Borgstrom et al, 2012;Skogstrand et al, 2012;Anbarasu et al, 2013;Armand et al, 2014;Essone et al, 2014); and extraction and culture of leukocytes from the peripheral blood of patients with active or latent TB infection and healthy individuals, followed by analysis of the differences insecreted cytokines before and after stimulation with TB-specific antigens (Rivera-Ordaz et al, 2012). However, consistent results have not been obtained using the aforementioned methods because the number of cytokines analyzed is often small and the types of assays usedto detect them vary between studies; thus, a more systematic approach for analyzing all cytokines is needed.…”

Section: Introductionmentioning

confidence: 99%

Regulation network of serum cytokines induced by tuberculosis-specific antigens reveals biomarkers for tuberculosis diagnosis

Wei¹,

Wu²,

Lin³

et al. 2015

Genet. Mol. Res.

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“…Use of product‐to‐substrate ratio may magnify effects not statistically apparent for either analyte alone. [ 58,65–66 ]…”

Section: Ido Activity Is Elevated During Human Tuberculosismentioning

confidence: 99%

Evolutionary Views of Tuberculosis: Indoleamine 2,3‐Dioxygenase Catalyzed Nicotinamide Synthesis Reflects Shifts in Macrophage Metabolism

et al. 2020

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Indoleamine 2,3-dioxygenase (IDO) is the rate-limiting enzyme in conversion of tryptophan to kynurenines, feeding de novo nicotinamide synthesis. IDO orchestrates materno-foetal tolerance, increasing human reproductive fitness. IDO mediates immune suppression through depletion of tryptophan required by T lymphocytes and other mechanisms. IDO is expressed by alternatively activated macrophages, suspected to play a key role in tuberculosis (TB) pathogenesis. Unlike its human host, Mycobacterium tuberculosis can synthesize tryptophan, suggesting possible benefit to the host from infection with the microbe. Intriguingly, nicotinamide analogues are used to treat TB. In reviewing this field, it is postulated that flux through the nicotinamide synthesis pathway reflects switching between aerobic glycolysis and oxidative phosphorylation in M. tuberculosis-infected macrophages. The evolutionary cause of such shifts may be ancient mitochondrial behavior related to reproductive fitness. Evolutionary perspectives on the IDO pathway may elucidate why, after centuries of co-existence with the Tubercle bacillus, humans still remain susceptible to TB disease.

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The discovery and identification of a candidate proteomic biomarker of active tuberculosis

Cited by 41 publications

References 32 publications

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Serum amyloid A, protein Z, and C4b-binding protein β chain as new potential biomarkers for pulmonary tuberculosis

Regulation network of serum cytokines induced by tuberculosis-specific antigens reveals biomarkers for tuberculosis diagnosis

Evolutionary Views of Tuberculosis: Indoleamine 2,3‐Dioxygenase Catalyzed Nicotinamide Synthesis Reflects Shifts in Macrophage Metabolism

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