The Discovery of Oropharyngeal Microbiota with Inhibitory Activity against Pathogenic Neisseria gonorrhoeae and Neisseria meningitidis: An In Vitro Study of Clinical Isolates

Akomoneh, Elvis Achondou; Laumen, Jolein; Abdellati, Saïd; Dijck, Christophe Van; Vanbaelen, Thibau; Xavier, Basil Britto; Manoharan-Basil, Sheeba Santhini; Kenyon, Chris

doi:10.3390/microorganisms10122497

Cited by 3 publications

(7 citation statements)

References 41 publications

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“…Previously, we identified a Rothia dentocariosa strain from oropharyngeal samples from men who have sex with men with inhibitory activity against pathogenic Neisseria spp. [25]. The crude extract derived from R. dentocariosa showed inhibitory activity against two pathogens, Neisseria gonorrhoeae and Neisseria meningitidis, as demonstrated in the spot-on-lawn assay (Figure 2).…”

Section: Discussionmentioning

confidence: 91%

“…This process led to the identification of a strain of Rothia dentocariosa that was inhibitory to the pathogenic Neisseria spp. [25].…”

Section: Samples Used In This Study and The Isolation And Identificat...mentioning

confidence: 99%

“…The antimicrobial activity of the crude extract was detected using the spot-on-lawn agar overlay assay protocol [25]. Briefly, 0.5 MacFarland of 24 h culture of target strains (N. gonorrhoeae or N. meningitidis) grown in GC broth was diluted in 4 mL of GC semi-solid medium pre-warmed to 55 • C to obtain a concentration of approximately 10 6 CFU/mL.…”

Section: Detection Of the Antimicrobial Activity Of The Crude Extractmentioning

confidence: 99%

“…Previously, we isolated and identified Rothia dentocariosa from oropharyngeal swabs of men who have sex with men (MSM) with inhibitory activities against Neisseria gonorrhoeae and N. meningitidis, the two human pathogenic species of the genus Neisseria [25]. Considering the continuing emergence of antimicrobial resistance reported from both these pathogens, alternative treatments are urgently required [26,27].…”

Section: Introductionmentioning

confidence: 99%

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Genome Mining Uncovers NRPS and PKS Clusters in Rothia dentocariosa with Inhibitory Activity against Neisseria Species

Akomoneh,

Gestels,

Abdellati

et al. 2023

Antibiotics

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The growing global threat of antimicrobial resistance is reaching a crisis point as common bacterial infections, including those caused by pathogenic Neisseria species, are becoming increasingly untreatable. This is compelling the scientific community to search for new antimicrobial agents, taking advantage of computational mining and using whole genome sequences to discover natural products from the human microbiome with antibiotic effects. In this study, we investigated the crude extract from a Rothia dentocariosa strain with demonstrated antimicrobial activity against pathogenic Neisseria spp. by spot-on-lawn assay. The genomic DNA of the R. dentocariosa strain was sequenced, and bioinformatic evaluation was performed using antiSMASH and PRISM to search for biosynthetic gene clusters (BGCs). The crude extract with potential antimicrobial activity was run on Tricine-SDS-PAGE, and the putative peptides were characterised using liquid chromatography–tandem mass spectrometry (LC-MS). The crude extract inhibited the growth of the pathogenic Neisseria spp. Six BGCs were identified corresponding to non-ribosomal peptide synthases (NRPSs), polyketide synthases (PKSs), and ribosomally synthesised and post-translationally modified peptides. Three peptides were also identified corresponding to Actinorhodin polyketide putative beta-ketoacyl synthase 1. These findings serve as a useful reference to facilitate the research and development of NRPS and PKS as antimicrobial products against multidrug-resistant N. gonorrhoeae.

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Section: Discussionmentioning

confidence: 91%

“…This process led to the identification of a strain of Rothia dentocariosa that was inhibitory to the pathogenic Neisseria spp. [25].…”

Section: Samples Used In This Study and The Isolation And Identificat...mentioning

confidence: 99%

Section: Detection Of the Antimicrobial Activity Of The Crude Extractmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genome Mining Uncovers NRPS and PKS Clusters in Rothia dentocariosa with Inhibitory Activity against Neisseria Species

Akomoneh,

Gestels,

Abdellati

et al. 2023

Antibiotics

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“…A number of bacterial species, such as numerous Enterobacteriales spp. that are prevalent in the anorectum inhibit the growth of N. gonorrhoeae through the production of substances such as bacteriocins [28][29][30]. Various streptococcal species have been noted to exhibit a similar effect [31].…”

Section: Discussionmentioning

confidence: 99%

The Prevalence of Antibiotic Tolerance in Neisseria gonorrhoeae Varies by Anatomical Site

Balduck,

Strikker,

Gestels

et al. 2024

Preprint

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Background: Bacteria can survive antibiotic exposure by mechanisms like tolerance without a shift in antimicrobial susceptibility. In this study, we investigated the presence of tolerance to three antimicrobials, ceftriaxone (CRO), azithromycin (AZM), and ciprofloxacin (CIP), in clinical isolates and WHO reference panel of N. gonorrhoeae and explored the prevalence between urogenital and anorectal infection sites. Methods: We examined 66 N. gonorrhoeae clinical isolates, evenly divided between anorectal and urogenital infection sites, using a modified tolerance disk (TD test) for three antibiotics. The isolates underwent a three-step incubation process wherein the isolates were exposed to an antibiotic disc for 20 hours of incubation (Step I), followed by the replacement of the antibiotic disc with a nutrient disc for overnight incubation (Step II) and additional overnight incubation with extra nutrients (Step III). Results: Four isolates were lost due to contamination. Of 62 clinical isolates, 7 exhibited tolerance to AZM, with 6 from anorectal and 1 from urogenital sites (p = 0.0496). Tolerance to CRO was observed in 11 isolates, which was significantly associated with the infection site, with 9 from the anorectum, and 2 from the urogenital site being tolerant (p = 0.02). Tolerance to CIP was found in 11 isolates, but was not significantly associated with the infection site. Tolerance was also detected in 8 (K, M, N, O, P, U, V, W) out of the 14 WHO (World Health Organization) reference strains, with varying patterns of tolerance to CRO (n=8), CIP (n=2) and AZM (n=1). Conclusion: This study identified CRO, AZM, and CIP tolerance in clinical Neisseria gonorrhoeae isolates. Significant variations in tolerance proportions between anorectal and urogenital infection sites, particularly for CRO, were observed. Additionally, tolerance was detected in WHO reference strains.

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The Prevalence of Antibiotic Tolerance in Neisseria gonorrhoeae Varies by Anatomical Site

Balduck,

Strikker,

Gestels

et al. 2024

Pathogens

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Background: Tolerance enables bacteria to survive intermittent antibiotic exposure without an increase in antimicrobial susceptibility. In this study, we investigated the presence of tolerance to three antimicrobials, ceftriaxone, azithromycin and ciprofloxacin, in clinical isolates and the WHO (World Health Organization) reference panel of Neisseria gonorrhoeae. Methods: We used the modified tolerance disk (TD test) to assess for tolerance to ceftriaxone, azithromycin and ciprofloxacin in 14 WHO reference strains and 62 N. gonorrhoeae clinical isolates—evenly divided between anorectal and urogenital infections. The isolates underwent a three-step incubation process wherein the isolates were exposed to an antibiotic disk for 20 h of incubation (Step I), followed by the replacement of the antibiotic disk with a nutrient disk for overnight incubation (Step II) and additional overnight incubation with extra nutrients (Step III). Results: A total of 4 of the 62 clinical anorectal isolates and none of the urogenital isolates exhibited tolerance to azithromycin (p = 0.033). Tolerance to ceftriaxone and ciprofloxacin was observed in eight and four isolates, respectively, with no difference between infection sites. Tolerance was also detected in 8 (K, M, N, O, P, U, V, W) out of the 14 WHO reference strains, with varying patterns of tolerance to ceftriaxone (n = 8), ciprofloxacin (n = 2) and azithromycin (n = 1). Conclusions: This study identified ceftriaxone, azithromycin and ciprofloxacin tolerance in clinical and WHO reference N. gonorrhoeae isolates. Azithromycin tolerance was more common in anorectal than urogenital infections.

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The Discovery of Oropharyngeal Microbiota with Inhibitory Activity against Pathogenic Neisseria gonorrhoeae and Neisseria meningitidis: An In Vitro Study of Clinical Isolates

Cited by 3 publications

References 41 publications

Genome Mining Uncovers NRPS and PKS Clusters in Rothia dentocariosa with Inhibitory Activity against Neisseria Species

Genome Mining Uncovers NRPS and PKS Clusters in Rothia dentocariosa with Inhibitory Activity against Neisseria Species

The Prevalence of Antibiotic Tolerance in Neisseria gonorrhoeae Varies by Anatomical Site

The Prevalence of Antibiotic Tolerance in Neisseria gonorrhoeae Varies by Anatomical Site

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