The discovery of Q-markers of Qiliqiangxin Capsule, a traditional Chinese medicine prescription in the treatment of chronic heart failure, based on a novel strategy of multi-dimensional “radar chart” mode evaluation

He, Liangliang; Liu, Yuehe; Yang, Kefeng; Zou, Zhiwei; Fan, Cai‐lian; Yao, Zhihong; Dai, Yi; Li, Keshen; Chen, Jiaxu; Yao, Xin‐Sheng

doi:10.1016/j.phymed.2020.153443

Cited by 38 publications

(22 citation statements)

References 12 publications

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“…Based on the scientific connotation of Q markers in TCM, the quality control indexes in chicory should meet the requirements of Q markers in terms of potency, specificity, quality transferability, measurability, and compatibility ( 32 ). The results of content analysis of seven components, including chlorogenic acid, esculetin, lactucin, cichoric acid, isochlorogenic acid A, 11β,13- dihydrolactucopicrin, and quercetin, in the bulk samples showed that the content of components in different parts of chicory medicinal plants varied.…”

Section: Resultsmentioning

confidence: 99%

Bioactive-Chemical Quality Markers Revealed: An Integrated Strategy for Quality Control of Chicory

Lin

et al. 2022

Front. Nutr.

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As a miraculous Xinjiang Uyghur customary traditional Chinese medicine (TCM), Chicory (Cichorium glandulosum Boiss.et Huet and Cichorium intybus L.) has been found to have therapeutic potential for metabolic diseases in recent years. Although it is widely used as an ethnic medicine, there is still a lack of targeted quality control indicators in quality standards. Hence, this study was conducted to further develop a strategy to reveal bioactive-chemical quality markers based on the existing foundation. First, through the comparative screening of fingerprint profiles of a large amount of Cichorium glandulosum Boiss.et Huet and Cichorium intybus L., superiority components were found to be potential indicators of chemical quantitative properties for the roots and above-ground parts. The results of content determination showed that their contents differed among different species and parts. Second, the potential dominant components were further confirmed using network pharmacology and molecular docking techniques. Again, the results of RAW264.7 cells and L02 cells experiments showed that chicory acid and lactucin were the main components that could reflect the anti-inflammatory and uric acid-lowering potential of chicory. Finally, under this strategy, this study reveals that cichoric acid and lactucin have the properties of quality markers and quality control of chicory. In a word, this work contributes to the quality control, standard improvement, and rational clinical use of chicory.

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Section: Resultsmentioning

confidence: 99%

Bioactive-Chemical Quality Markers Revealed: An Integrated Strategy for Quality Control of Chicory

Lin

et al. 2022

Front. Nutr.

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“…Accordingly, natural products may provide a new direction for developing anti‐hypertrophic drugs. Our previous research has shown that PM from Periplocae cortex could be absorbed into the blood of rats and reduced ISO‐induced myocardial hypertrophy 33,34 …”

Section: Discussionmentioning

confidence: 99%

“…Our previous research has shown that PM from Periplocae cortex could be absorbed into the blood of rats and reduced ISO-induced myocardial hypertrophy. 33,34 Therefore, PM is considered a potential therapeutic compound to alleviate cardiac hypertrophy. In order to develop this active compound, the efficacy and mechanism of PM in improving cardiac hypertrophy was investigated.…”

Section: Pm Inhibited Nuclear Translocation Of Stat3 In H9c2 Cellsmentioning

confidence: 99%

Periplocymarin alleviates pathological cardiac hypertrophy via inhibiting the JAK2/STAT3 signalling pathway

Fan

Liang

et al. 2022

J Cellular Molecular Medi

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Pathological cardiac hypertrophy is the most important risk factor for developing chronic heart failure. Therefore, the discovery of novel agents for treating pathological cardiac hypertrophy remains urgent. In the present study, we examined the therapeutic effect and mechanism of periplocymarin (PM)‐mediated protection against pathological cardiac hypertrophy using angiotensinII (AngII)‐stimulated cardiac hypertrophy in H9c2 cells and transverse aortic constriction (TAC)‐induced cardiac hypertrophy in mice. In vitro, PM treatment significantly reduced the surface area of H9c2 cells and expressions of hypertrophy‐related proteins. Meanwhile, PM markedly down‐regulated AngII‐induced translocation of p‐STAT3 into the nuclei and enhanced the phosphorylation levels of JAK2 and STAT3 proteins. The STAT3 specific inhibitor S3I‐201 or siRNA‐mediated depleted expression could alleviate AngII‐induced cardiac hypertrophy in H9c2 cells following PM treatment; however, PM failed to reduce the expressions of hypertrophy‐related proteins and phosphorylated STAT3 in STAT3‐overexpressing cells, indicating that PM protected against AngII‐induced cardiac hypertrophy by modulating STAT3 signalling. In vivo, PM reversed TAC‐induced cardiac hypertrophy, as determined by down‐regulating ratios of heart weight to body weight (HW/BW), heart weight to tibial length (HW/TL) and expressions of hypertrophy‐related proteins accompanied by the inhibition of the JAK2/STAT3 pathway. These results revealed that PM could effectively protect the cardiac structure and function in experimental models of pathological cardiac hypertrophy by inhibiting the JAK2/STAT3 signalling pathway. PM is expected to be a potential lead compound of the novel agents for treating pathological cardiac hypertrophy.

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“…The selection criteria of 14 quantitative components included four factors: (1) detected in QLQX [7] and derived from the main herbals, (2) highly exposed to plasma by UHPLC-Q-TOF-MS in our previous study [8], (3) exhibited anti-heart failure activity in H9c2 cardiomyocytes in our previous study [9], and (4) identified as toxic components [15][16][17]. In the present study, several bioactive components were not selected, including hydroxysafflor yellow A from Carthami Flos [19] and salvianolic acids from Salvia Miltiorrhiza Radix et Rhizoma [20].…”

Section: Selection Of 14 Bioactive Components and Methods Developmentmentioning

confidence: 99%

“…Our investigation on the metabolic profiles of QLQX showed that cardiac glycosides, triterpenes, saponins, flavonoids, and alkaloids were the main components present in rat plasma [8]. Among them, sinapine thiocyanate, fuziline, neoline, benzoylhypaconine, benzoylmesaconine, benzoylaconine, periplocin, periplocymarin, formononetin, astragaloside IV, ginsenoside Rb1, and alisol A exhibited anti‐heart failure activity in H9c2 cardiomyocytes [9]. Besides, calycosin possessed its antioxidant properties as well as the treatment for cardiovascular disease [10], while hypaconitine exhibited cardioprotective effects [11].…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics, hepatic disposition, and heart tissue distribution of 14 compounds in rat after oral administration of Qi‐Li‐Qiang‐Xin capsule via ultra‐high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry

Tang

Dai

Zeng

et al. 2022

J of Separation Science

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In the present study, a specific and sensitive approach using ultra-highperformance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was developed and validated for the quantitative analysis of 14 constituents in rat plasma, liver, and heart. The method was fully validated and successfully applied to pharmacokinetic, hepatic disposition, and heart tissue distribution studies of 14 compounds after the oral administration of Qi-Li-Qiang-Xin capsule. Ginsenoside Rb1, alisol A, astragaloside IV, and periplocymarin were found to be highly exposed in rat plasma, while toxic components such as hypaconitine, mesaconitine, and periplocin had low circulation levels in vivo. Moreover, sinapine thiocyanate, neoline, formononetin, calycosin, and alisol A exhibited significant liver first-pass effects. Notably, high levels of alisol A, periplocymarin, benzoylmesaconine, and benzoylhypaconine were observed in the heart. Based on high exposure and appropriate pharmacokinetic features in the systemic plasma and heart, astragaloside IV, ginsenoside Rb1, periplocymarin, benzoylmesaconine, benzoylhypaconine, and alisol A can be considered as the main potentially effective components. Ultimately, the results provide relevant information for discovery of effective substances, as well as further anti-heart failure action mechanism investigations of Qi-Li-Qiang-Xin capsule.

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The discovery of Q-markers of Qiliqiangxin Capsule, a traditional Chinese medicine prescription in the treatment of chronic heart failure, based on a novel strategy of multi-dimensional “radar chart” mode evaluation

Cited by 38 publications

References 12 publications

Bioactive-Chemical Quality Markers Revealed: An Integrated Strategy for Quality Control of Chicory

Bioactive-Chemical Quality Markers Revealed: An Integrated Strategy for Quality Control of Chicory

Periplocymarin alleviates pathological cardiac hypertrophy via inhibiting the JAK2/STAT3 signalling pathway

Pharmacokinetics, hepatic disposition, and heart tissue distribution of 14 compounds in rat after oral administration of Qi‐Li‐Qiang‐Xin capsule via ultra‐high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry

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