The Discovery of Vibrio harveyi on Litopenaeus vannamei Infected White Feces Disease in Situbondo, East Java

Abstract

This research was conducted to discover Vibrio harveyi infected vannamei shrimp with White Feces Disease (WFD) in Situbondo, East Java Province. This research was conducted in November 2017 until September 2018. All Vibrio isolates from shrimp infected WFD were identified with biochemical tests, Analytical Profile Index/ API 20NE (BioMeriuex), and PCR with specific primers for V. harveyi. Additional parameters were the water quality, plankton brackishwater pond abundance, and antibiotic resistance test. Result showed that from 17 bacterial isolates identified, 10 isolates were V. harveyi (58.82%), three isolates were V. alginolyticus (17.65%), one V. fluvialis isolate (5.88%), one V. parahaemolyticus isolate (5.88%), and two non Vibrio isolates which were identified as Shewanella putrefaciens (11.76%). All isolates of V. harveyi also showed resistance activity on more than one antibiotic. Poor water quality had been identified as abnormal result of pH, alkalinity, salinity, ammonia levels and total organic matter level. Plankton abundance observation showed that Chloropyceae, Diatom, and Dinoflagellata dominated all sampled brackishwater ponds. This research concluded that V. harveyi were discovered on vannamei shrimp with poor water quality and plankton abundance in the pond samples.

The presence of disease in growing spiny lobster Panulirus spp. in floating net cages at Tanjung Putus Island, Lampung

“…Testing the bacterial sample to be tested is gram negative which is a non-Enterobacteriaceae species so that the KIT API 20 NE (Biomerieux) type is used (Sumini & Kusdarwati, 2020). The results obtained for the bacterial samples Lobster one, Lobster two, and Lobster four, namely Vibrio parahaemolyticus with %ID 99.9%, 99.9%, and 99.7%, respectively.…”

ANALISIS KOMPOSISI DAN KELIMPAHAN BAKTERI Vibrio sp. PADA BUDIDAYA UDANG VANNAMEI (Litopenaeus vannamei) SISTEM INTENSIF

“…Penyakit yang sering menyerang udang vannamei yang dibudidaya salah satunya penyakit vibrosis. Penyakit ini bersifat oportunistik, sangat ganas dan berbahaya, yang dapat menyebabkan rendahnya tingkat kehidupan (survival rate) pada hewan budidaya, sebagian besar kasus kemunculan penyakit ini yang disebabkan oleh buruknya kualitas air (Sumini & Kusdarwati, 2020;Ariadi & Mujtahidah, 2022). Bakteri Vibrio sp.…”

Antimicrobial Potential of Sonneratia alba and Sonneratia caseolaris against Shrimp Pathogens

“…White feces disease (WFD) is caused by bacteria from the genus Vibrio such as V. parahaemolyticus, V. fluvialis, V. vulnificus, V. mimicus, V. alginolyticus, V. cholera [4] and V. Harveyi [5]. This disease can cause mortality in L. vannamei shrimp as much as 30% [4].…”

“…This disease can cause mortality in L. vannamei shrimp as much as 30% [4]. Symptoms of this disease are discoloration of hepatopancreas and white feces, floating on the water surface, and white intestine [5]. White spot syndrome virus (WSSV) has caused mass mortality in the shrimp culture industry.…”