The disintegration of soybean by a cellulase preparation from Irpex lacteus Fr. and the enzyme relating to it.

Kawai, Masanobu; Katsumata, Ryoichi; Tsuruta, Teruyuki; Shimura, Gen; Suga, Yoshito; Samejima, Hirotoshi

doi:10.1271/bbb1961.43.1855

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“…The time course of elaboration demonstrated the typical lag phase required for the induction of the synthetic apparatus as reported by Catley for whole cells (7). In this experiment, the protoplasts were incubated in the presence of the lytic complex Driselase (22). An identical time course of elaboration was observed in the absence of Driselase (data not shown).…”

Section: Resultsmentioning

confidence: 56%

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Pullulan Elaboration by Aureobasidium pullulans Protoplasts

Finkelman

Vardanis

1982

Appl Environ Microbiol

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Protoplasts of Aureobasidium pullulans are capable of producing pullulan. Biosynthesis of the polymer pullulan required induction with kinetics similar to those of whole cells. The protoplasts also produced a heteropolysaccharide component containing mannose, glucose, and galactose. The relative proportions of the pullulan and heteropolysaccharide fractions were a function of glucose concentration, with the pullulan content of the total polysaccharide rising from 20% at 2.5 mM glucose to 45% at 20 mM glucose. Elaboration of pullulan by both cells and protoplasts was sensitive to 0.6 M KCl, which was present as the osmotic stabilizer in protoplast experiments. The presence of KCl resulted in a shift in the pH optimum to a more acidic value. The molecular weight of the protoplast-derived pullulan was sharply reduced from the molecular weight of the whole-cell-derived product. Exposure of the protoplasts to proteolytic enzymes had no effect on polysaccharide elaboration.

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Section: Resultsmentioning

confidence: 56%

“…The experiment further demonstrated that these polysaccharides were not sensitive to the enzymes of the whole Driselase complex. The insensitivity of pullulan to several fractions of the Driselase complex has been reported by Kawai et al (22).…”

Section: Resultsmentioning

confidence: 61%

Pullulan Elaboration by Aureobasidium pullulans Protoplasts

Finkelman

Vardanis

1982

Appl Environ Microbiol

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show abstract

The disintegration of soybean by a cellulase preparation from Irpex lacteus Fr. and the enzyme relating to it.

Cited by 1 publication

References 1 publication

Pullulan Elaboration by Aureobasidium pullulans Protoplasts

Pullulan Elaboration by Aureobasidium pullulans Protoplasts

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